safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	7	17.83

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
MOBFGIJG_12	604732	605586	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
MOBFGIJG_12	586023	587225	blt	ARO:3003006	blt	100.00	100.00	L32599.1
MOBFGIJG_15	585254	585592	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
MOBFGIJG_15	584937	585254	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
MOBFGIJG_16	390708	392351	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
MOBFGIJG_12	363718	364887	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
MOBFGIJG_17	276552	279122	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
MOBFGIJG_16	162100	162693	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
MOBFGIJG_16	111597	113036	lmrB	ARO:3002813	lmrB	99.79	100.42	JYFL01000006.1
MOBFGIJG_16	98782	99702	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
MOBFGIJG_12	604732	605586	aadK	Streptomycin	100.00	100.00	M26879
MOBFGIJG_16	98782	99702	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
MOBFGIJG_1	1004904	1006280	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
MOBFGIJG_12	604735	605586	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
MOBFGIJG_16	98782	99699	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
MOBFGIJG_1	1002386	1002904	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
MOBFGIJG_1	1004907	1006280	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
MOBFGIJG_16	390711	392351	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
MOBFGIJG_12	54786	57380	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
MOBFGIJG_1	519092	520234	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
MOBFGIJG_1	520717	521898	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
MOBFGIJG_1	520253	520702	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
MOBFGIJG_12	8128	9891	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
MOBFGIJG_1	112782	113567	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
MOBFGIJG_1	108946	109884	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
MOBFGIJG_1	111560	112756	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
MOBFGIJG_1	109912	111531	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
MOBFGIJG_1	101790	108926	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
MOBFGIJG_12	164351	164992	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
MOBFGIJG_18	27975	26839	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
MOBFGIJG_20	4231	5100	pdxS	1.10e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
MOBFGIJG_15	277546	276770	CodY	8.17e-125	nutrient-responsive regulator	81.08	100.00	ACJ80679
MOBFGIJG_1	367505	368074	ClpP	6.27e-99	part of proteolytic complex	81.05	97.44	BAB94595
MOBFGIJG_15	734415	734023	spxA1	2.42e-69	redox-responsive transcription factor	83.21	100.00	AAF21893
MOBFGIJG_19	20512	20754	SpoVG	3.89e-38	RNA-binding protein	80.25	79.41	AAT02994
MOBFGIJG_16	345236	345433	CspA	1.82e-31	cold shock protein	90.91	100.00	CAA62903

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MOBFGIJG_1	0.0	1.0	0.0
MOBFGIJG_12	0.0	1.0	0.0
MOBFGIJG_15	0.0	1.0	0.0
MOBFGIJG_16	0.0	1.0	0.0
MOBFGIJG_17	0.0	1.0	0.0
MOBFGIJG_18	0.0	1.0	0.0
MOBFGIJG_19	0.0	1.0	0.0
MOBFGIJG_20	0.0	1.0	0.0
MOBFGIJG_21	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
MOBFGIJG_12	536095	566339	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600
MOBFGIJG_15	615431	641539	False	Myoviridae	VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667

Phigaro v2.4.0 (Database: Jan. 2024 release)