safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	0	1	1.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
NBPJEPGP_1	2898495	2897791	WalR	2.21e-111	transcriptional regulatory protein	83.40	100.00	EPH95667

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
NBPJEPGP_1	0.0	1.0	0.0
NBPJEPGP_2	1.0	0.0	1.0
NBPJEPGP_3	1.0	0.0	1.0
NBPJEPGP_4	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
NBPJEPGP_1	836721	868346	False	Siphoviridae	VOG3304, VOG0862, VOG5353, VOG4552, VOG0181, VOG10867, VOG4600, VOG1309, VOG4566, VOG0189, VOG9082, VOG5998, VOG5562, VOG4693, VOG0744, VOG4673, VOG5616, VOG4841, VOG0519, VOG4544, VOG4556, VOG4573, VOG4553, VOG0204, VOG4589, VOG1898, VOG4545, VOG4633, VOG4763, VOG3498, VOG0648, VOG1637, VOG0054, VOG0703
NBPJEPGP_1	953863	983966	False	Siphoviridae	VOG0181, VOG10867, VOG1302, VOG4967, VOG0321, VOG4548, VOG0322, VOG6545, VOG4632, VOG4609, VOG0327, VOG5998, VOG6795, VOG5562, VOG0198, VOG6154, VOG4581, VOG4556, VOG4568, VOG7454, VOG3471, VOG4589, VOG1900, VOG0704, VOG4586, VOG1049, VOG3474, VOG5793, VOG4633, VOG4763, VOG3498, VOG0648, VOG0861, VOG5087, VOG0602, VOG4649
NBPJEPGP_1	1161055	1190908	False	Siphoviridae	VOG0283, VOG0226, VOG4566, VOG0189, VOG6623, VOG4693, VOG2820, VOG3643, VOG3643, VOG0198, VOG3490, VOG4570, VOG4549, VOG3699, VOG10227, VOG0720, VOG4564, VOG4555, VOG0694, VOG1322, VOG4713, VOG1320, VOG9583, VOG1321, VOG0799, VOG0725, VOG1323, VOG0801, VOG4619, VOG4683, VOG0861, VOG7017
NBPJEPGP_1	1656732	1690929	False	Siphoviridae	VOG3801, VOG4918, VOG0703, VOG7017, VOG0861, VOG4660, VOG4828, VOG4633, VOG0083, VOG2225, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG10868, VOG4553, VOG4568, VOG4556, VOG3644, VOG4544, VOG4581, VOG0944, VOG5532, VOG4570, VOG4892, VOG0198, VOG6140, VOG4813, VOG7735, VOG0025, VOG4799, VOG3642, VOG10867, VOG0181, VOG4552
NBPJEPGP_1	2976210	2986357	False	Siphoviridae	VOG1581, VOG4632, VOG4609, VOG4581, VOG4544, VOG5051, VOG4556, VOG4568, VOG0204, VOG4589