Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 14 | 10 | 4 | 17.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BJAOBIIB_1 | 2620204 | 2621058 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| BJAOBIIB_1 | 2601467 | 2602669 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| BJAOBIIB_1 | 2377971 | 2379140 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| BJAOBIIB_1 | 1395757 | 1396074 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BJAOBIIB_1 | 1395419 | 1395757 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BJAOBIIB_1 | 933712 | 936282 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| BJAOBIIB_1 | 635421 | 637064 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| BJAOBIIB_1 | 393451 | 394044 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| BJAOBIIB_1 | 342931 | 344364 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| BJAOBIIB_1 | 330118 | 331038 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| BJAOBIIB_1 | 121509 | 125090 | Bacillus subtilis rpoB mutants conferring resistance to rifampin | ARO:3007073 | Bsub_rpoB_RIF | 99.92 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BJAOBIIB_1 | 330118 | 331038 | mph(K) | Spiramycin, Telithromycin | 99.89 | 100.00 | NC_000964 |
| BJAOBIIB_1 | 4074579 | 4075955 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.06 | 100.00 | D12567 |
| BJAOBIIB_1 | 2620206 | 2621058 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BJAOBIIB_1 | 330118 | 331035 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| BJAOBIIB_1 | 4074582 | 4075955 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| BJAOBIIB_1 | 635424 | 637064 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| BJAOBIIB_1 | 2620207 | 2621058 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| BJAOBIIB_1 | 4072027 | 4072545 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| BJAOBIIB_1 | 2069066 | 2071660 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.03 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BJAOBIIB_1 | 3181147 | 3182085 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| BJAOBIIB_1 | 3594057 | 3595238 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| BJAOBIIB_1 | 3184983 | 3185768 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| BJAOBIIB_1 | 3592432 | 3593574 | capA | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | NP_391469 |
| BJAOBIIB_1 | 2178604 | 2179245 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| BJAOBIIB_1 | 3593593 | 3594042 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| BJAOBIIB_1 | 2023642 | 2025405 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| BJAOBIIB_1 | 3183761 | 3184957 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| BJAOBIIB_1 | 3173991 | 3181127 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.96 | 100.00 | NP_391076 |
| BJAOBIIB_1 | 3182113 | 3183732 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.89 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BJAOBIIB_1 | 132472 | 133608 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BJAOBIIB_1 | 18665 | 19534 | pdxS | 3.05e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BJAOBIIB_1 | 1709082 | 1709858 | CodY | 3.33e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BJAOBIIB_1 | 3440835 | 3441404 | ClpP | 2.47e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BJAOBIIB_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BJAOBIIB_1 | 169213 | 177063 | False | Unknown | VOG3549, VOG1878, VOG0083, VOG1309, VOG1292, VOG11060, VOG9600 |
| BJAOBIIB_1 | 180251 | 214222 | False | Myoviridae | VOG10058, VOG0198, VOG6242, VOG4618, VOG4567, VOG1328, VOG5361, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG5668, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG10120, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705, VOG0753 |
| BJAOBIIB_1 | 1339472 | 1365580 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| BJAOBIIB_1 | 2550675 | 2572882 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773 |
Phigaro v2.4.0 (Database: Jan. 2024 release)