Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 9 | 0 | 1 | 9.06 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IEBFHEGP_11 | 133282 | 134487 | Mef(En2) | ARO:3004659 | Mef(En2) | 99.50 | 100.00 | AF251288.1 |
| IEBFHEGP_19 | 35728 | 36528 | ErmF | ARO:3000498 | ErmF | 99.62 | 100.00 | M17124.1 |
| IEBFHEGP_30 | 7307 | 9253 | tet(Q) | ARO:3000191 | tet(Q) | 96.55 | 98.63 | Z21523.1 |
| IEBFHEGP_20 | 2834 | 3649 | sul2 | ARO:3000412 | sul2 | 100.00 | 100.00 | AY055428.1 |
| IEBFHEGP_13 | 235 | 1374 | tet(X1) | ARO:3005166 | tet(X1) | 99.72 | 105.57 | AJ311171.1 |
| IEBFHEGP_56 | 140 | 1306 | tet(X) | ARO:3000205 | tet(X) | 99.74 | 100.00 | M37699.1 |
| IEBFHEGP_4 | 114 | 977 | aadS | ARO:3004683 | aadS | 100.00 | 100.00 | M72415.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IEBFHEGP_19 | 35728 | 36528 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | M17808 |
| IEBFHEGP_20 | 2834 | 3649 | sul2 | Sulfamethoxazole | 100.00 | 100.00 | AY034138 |
| IEBFHEGP_56 | 140 | 1306 | tet(X) | Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.91 | 100.00 | M37699 |
| IEBFHEGP_30 | 7341 | 9253 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 97.18 | 99.33 | L33696 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IEBFHEGP_4 | 114 | 974 | aadS | aminoglycoside 6-adenylyltransferase AadS | EXACTX | 100.00 | 100.00 | WP_003013318.1 |
| IEBFHEGP_12 | 46120 | 47025 | blaMUN-1 | MUN family extended-spectrum class A beta-lactamase MUN-1 | ALLELEX | 100.00 | 100.00 | WP_206340447.1 |
| IEBFHEGP_19 | 35728 | 36525 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | EXACTX | 100.00 | 100.00 | WP_002682030.1 |
| IEBFHEGP_11 | 134512 | 135021 | lnu(AN2) | lincosamide nucleotidyltransferase Lnu(AN2) | EXACTX | 100.00 | 100.00 | WP_004308783.1 |
| IEBFHEGP_20 | 2837 | 3649 | sul2 | sulfonamide-resistant dihydropteroate synthase Sul2 | EXACTX | 100.00 | 100.00 | WP_001043260.1 |
| IEBFHEGP_13 | 238 | 1374 | tet(X1) | tetracycline-inactivating monooxygenase Tet(X1) | BLASTX | 99.74 | 100.00 | WP_204376222.1 |
| IEBFHEGP_56 | 143 | 1306 | tet(X2) | tetracycline-inactivating monooxygenase Tet(X2) | BLASTX | 99.74 | 100.00 | WP_008651082.1 |
| IEBFHEGP_11 | 133282 | 134484 | mef(En2) | macrolide efflux MFS transporter Mef(En2) | BLASTX | 99.50 | 100.00 | WP_063853729.1 |
| IEBFHEGP_30 | 7340 | 9253 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.06 | 99.53 | WP_063856409.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IEBFHEGP_54 | 132511 | 134091 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 86.91 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IEBFHEGP_1 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_2 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_3 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_4 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_5 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_6 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_7 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_8 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_9 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_10 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_11 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_12 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_13 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_14 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_15 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_16 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_17 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_18 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_19 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_20 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_21 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_22 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_23 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_24 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_25 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_26 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_27 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_28 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_29 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_30 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_31 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_32 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_33 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_34 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_35 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_37 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_38 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_39 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_40 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_41 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_42 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_43 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_44 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_45 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_46 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_47 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_48 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_49 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_50 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_51 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_52 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_53 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_54 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_55 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_56 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_57 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_58 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_59 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_60 | 1.0 | 0.0 | 1.0 |
| IEBFHEGP_61 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_62 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_63 | 0.0 | 1.0 | 0.0 |
| IEBFHEGP_64 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IEBFHEGP_1 | 121753 | 139435 | True | Siphoviridae | VOG0275, |
Phigaro v2.4.0 (Database: Jan. 2024 release)