safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	8	18.25

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
BKNCDDOJ_1	590817	591155	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
BKNCDDOJ_1	590500	590817	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
BKNCDDOJ_18	440141	440995	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
BKNCDDOJ_18	421432	422634	blt	ARO:3003006	blt	100.00	100.00	L32599.1
BKNCDDOJ_18	247147	248316	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
BKNCDDOJ_19	162826	163419	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
BKNCDDOJ_19	112323	113762	lmrB	ARO:3002813	lmrB	99.79	100.42	JYFL01000006.1
BKNCDDOJ_19	99508	100428	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
BKNCDDOJ_23	47355	48998	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
BKNCDDOJ_20	27249	29819	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
BKNCDDOJ_18	440141	440995	aadK	Streptomycin	100.00	100.00	M26879
BKNCDDOJ_19	99508	100428	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
BKNCDDOJ_22	36260	37636	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
BKNCDDOJ_18	440144	440995	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
BKNCDDOJ_19	99508	100425	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
BKNCDDOJ_22	39636	40154	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
BKNCDDOJ_22	36260	37633	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
BKNCDDOJ_23	47358	48998	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
BKNCDDOJ_21	54786	57380	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BKNCDDOJ_12	519092	520234	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
BKNCDDOJ_12	520717	521898	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
BKNCDDOJ_12	520253	520702	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
BKNCDDOJ_21	8128	9891	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
BKNCDDOJ_12	112782	113567	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
BKNCDDOJ_12	108946	109884	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
BKNCDDOJ_12	111560	112756	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
BKNCDDOJ_12	109912	111531	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
BKNCDDOJ_12	101790	108926	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
BKNCDDOJ_18	47792	48433	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BKNCDDOJ_24	27975	26839	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
BKNCDDOJ_3	11182	10313	pdxS	1.10e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
BKNCDDOJ_1	277545	276769	CodY	8.24e-125	nutrient-responsive regulator	81.08	100.00	ACJ80679
BKNCDDOJ_12	367505	368074	ClpP	5.52e-99	part of proteolytic complex	81.05	97.44	BAB94595
BKNCDDOJ_1	739978	739586	spxA1	2.44e-69	redox-responsive transcription factor	83.21	100.00	AAF21893
BKNCDDOJ_2	34575	34333	SpoVG	2.72e-38	RNA-binding protein	80.25	79.41	AAT02994
BKNCDDOJ_23	1883	2080	CspA	3.38e-32	cold shock protein	90.91	100.00	CAA62903
BKNCDDOJ_18	60395	60589	CspD	5.62e-28	cold shock protein	81.54	98.48	CAC99957

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BKNCDDOJ_1	0.0	1.0	0.0
BKNCDDOJ_2	0.0	1.0	0.0
BKNCDDOJ_3	0.0	1.0	0.0
BKNCDDOJ_4	0.0	1.0	0.0
BKNCDDOJ_5	0.0	1.0	0.0
BKNCDDOJ_12	0.0	1.0	0.0
BKNCDDOJ_18	0.0	1.0	0.0
BKNCDDOJ_19	0.0	1.0	0.0
BKNCDDOJ_20	0.0	1.0	0.0
BKNCDDOJ_21	0.0	1.0	0.0
BKNCDDOJ_22	0.0	1.0	0.0
BKNCDDOJ_23	0.0	1.0	0.0
BKNCDDOJ_24	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BKNCDDOJ_1	620994	647102	False	Myoviridae	VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667
BKNCDDOJ_18	565194	596618	False	Siphoviridae	VOG1013, VOG11038, VOG0641, VOG7896, VOG3390, VOG4612, VOG4660, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG1266, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0531, VOG2545, VOG0376, VOG0198, VOG1485, VOG7372, VOG2752

Phigaro v2.4.0 (Database: Jan. 2024 release)