Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EDBEALGE_23 | 471694 | 472548 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| EDBEALGE_23 | 452985 | 454187 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| EDBEALGE_1 | 424739 | 425056 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| EDBEALGE_1 | 424401 | 424739 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EDBEALGE_34 | 276567 | 279137 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| EDBEALGE_23 | 230666 | 231835 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| EDBEALGE_45 | 107960 | 108880 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| EDBEALGE_45 | 94626 | 96065 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| EDBEALGE_2 | 47355 | 48998 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| EDBEALGE_45 | 44970 | 45563 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EDBEALGE_23 | 471694 | 472548 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| EDBEALGE_45 | 107960 | 108880 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| EDBEALGE_78 | 90371 | 91747 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EDBEALGE_23 | 471697 | 472548 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| EDBEALGE_45 | 107963 | 108880 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| EDBEALGE_78 | 87853 | 88371 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| EDBEALGE_78 | 90374 | 91747 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| EDBEALGE_2 | 47358 | 48998 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| EDBEALGE_67 | 54786 | 57380 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EDBEALGE_12 | 519118 | 520260 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| EDBEALGE_12 | 520743 | 521924 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| EDBEALGE_12 | 520279 | 520728 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| EDBEALGE_67 | 8128 | 9891 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| EDBEALGE_12 | 112782 | 113567 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| EDBEALGE_12 | 108946 | 109884 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| EDBEALGE_12 | 111560 | 112756 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| EDBEALGE_12 | 109912 | 111531 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| EDBEALGE_12 | 101790 | 108926 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391076 |
| EDBEALGE_23 | 31320 | 31961 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EDBEALGE_3 | 31638 | 32774 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EDBEALGE_9 | 4231 | 5100 | pdxS | 1.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EDBEALGE_1 | 738003 | 738779 | CodY | 8.24e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EDBEALGE_12 | 367506 | 368075 | ClpP | 5.52e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| EDBEALGE_1 | 275578 | 275970 | spxA1 | 2.44e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| EDBEALGE_4 | 34575 | 34333 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| EDBEALGE_2 | 1883 | 2080 | CspA | 3.37e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| EDBEALGE_23 | 43923 | 44117 | CspD | 5.59e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EDBEALGE_1 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_2 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_3 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_4 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_5 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_6 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_7 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_8 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_9 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_10 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_11 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_12 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_13 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_14 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_15 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_16 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_17 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_18 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_19 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_20 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_21 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_22 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_23 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_24 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_25 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_26 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_27 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_28 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_29 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_30 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_32 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_33 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_34 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_35 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_36 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_37 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_38 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_39 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_40 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_42 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_43 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_44 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_45 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_46 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_47 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_48 | 1.0 | 0.0 | 1.0 |
| EDBEALGE_56 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_67 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_78 | 0.0 | 1.0 | 0.0 |
| EDBEALGE_89 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EDBEALGE_1 | 368454 | 394562 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| EDBEALGE_23 | 403056 | 433300 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600 |
| EDBEALGE_89 | 9436 | 33084 | False | Siphoviridae | VOG0221, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852, VOG6163, VOG4605, VOG4599, VOG9244, VOG10944, VOG1024 |
| EDBEALGE_89 | 52498 | 65596 | False | Unknown | VOG0052, VOG11080, VOG2352, VOG0397, VOG1198, VOG4561, VOG4714, VOG2352, VOG3851, VOG2725 |
Phigaro v2.4.0 (Database: Jan. 2024 release)