Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KEKFKBCE_49 | 63223 | 63540 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| KEKFKBCE_49 | 62885 | 63223 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| KEKFKBCE_31 | 59762 | 62332 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| KEKFKBCE_96 | 41810 | 42730 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| KEKFKBCE_96 | 28476 | 29915 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| KEKFKBCE_23 | 26403 | 27257 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| KEKFKBCE_29 | 19222 | 19815 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| KEKFKBCE_23 | 9306 | 10508 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| KEKFKBCE_69 | 7052 | 8698 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KEKFKBCE_23 | 26403 | 27257 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| KEKFKBCE_96 | 41810 | 42730 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KEKFKBCE_96 | 41822 | 42730 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| KEKFKBCE_23 | 26406 | 27257 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| KEKFKBCE_69 | 7055 | 8698 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| KEKFKBCE_64 | 40774 | 41292 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KEKFKBCE_54 | 29761 | 30942 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| KEKFKBCE_17 | 25096 | 25881 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| KEKFKBCE_54 | 28136 | 29278 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| KEKFKBCE_17 | 29737 | 36873 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| KEKFKBCE_32 | 1885 | 3648 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| KEKFKBCE_17 | 28779 | 29717 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| KEKFKBCE_17 | 25907 | 27103 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| KEKFKBCE_59 | 38076 | 38717 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| KEKFKBCE_54 | 29297 | 29746 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| KEKFKBCE_17 | 27132 | 28751 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KEKFKBCE_35 | 34483 | 35619 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KEKFKBCE_26 | 4517 | 5386 | pdxS | 2.00e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| KEKFKBCE_58 | 152614 | 153390 | CodY | 2.00e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KEKFKBCE_50 | 46018 | 45449 | ClpP | 2.98e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| KEKFKBCE_51 | 42354 | 41962 | spxA1 | 4.23e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| KEKFKBCE_51 | 42354 | 41962 | spx | 2.53e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| KEKFKBCE_6 | 34800 | 34558 | SpoVG | 7.54e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| KEKFKBCE_61 | 14986 | 15183 | CspA | 2.46e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| KEKFKBCE_59 | 26846 | 26652 | CspD | 4.40e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| KEKFKBCE_27 | 31221 | 31027 | CspB | 4.53e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KEKFKBCE_4 | 1.0 | 0.0 | 1.0 |
| KEKFKBCE_6 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_8 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_9 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_10 | 1.0 | 0.0 | 1.0 |
| KEKFKBCE_11 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_15 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_16 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_17 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_18 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_19 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_20 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_21 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_22 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_23 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_24 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_25 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_26 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_27 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_28 | 1.0 | 0.0 | 1.0 |
| KEKFKBCE_29 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_30 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_31 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_32 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_33 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_34 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_35 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_39 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_40 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_41 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_42 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_43 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_45 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_46 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_47 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_48 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_49 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_50 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_51 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_52 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_53 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_54 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_55 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_56 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_57 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_58 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_59 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_60 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_61 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_62 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_63 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_64 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_65 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_67 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_68 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_69 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_70 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_71 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_72 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_73 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_74 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_75 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_76 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_77 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_78 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_79 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_80 | 1.0 | 0.0 | 1.0 |
| KEKFKBCE_81 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_82 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_83 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_84 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_85 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_86 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_87 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_88 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_89 | 1.0 | 0.0 | 1.0 |
| KEKFKBCE_90 | 1.0 | 0.0 | 1.0 |
| KEKFKBCE_91 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_92 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_93 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_94 | 1.0 | 0.0 | 1.0 |
| KEKFKBCE_95 | 1.0 | 0.0 | 1.0 |
| KEKFKBCE_96 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_97 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_98 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_99 | 0.0 | 1.0 | 0.0 |
| KEKFKBCE_100 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KEKFKBCE_20 | 4946 | 15476 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| KEKFKBCE_49 | 5099 | 33421 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4843, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)