Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 7 | 7.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IEBOJFGD_19 | 53967 | 55016 | clbA | ARO:3002814 | clbA | 99.71 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IEBOJFGD_19 | 53981 | 55016 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 89.09 | 98.67 | KR610408 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IEBOJFGD_19 | 53970 | 55016 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.71 | 100.00 | WP_012116915.1 |
| IEBOJFGD_12 | 137827 | 140421 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.00 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IEBOJFGD_9 | 179568 | 180749 | capB | 0.0 | Capsule | Immune modulation | 81.79 | 99.66 | NP_391471 |
| IEBOJFGD_9 | 179104 | 179553 | capC | 1.67e-116 | Capsule | Immune modulation | 83.81 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IEBOJFGD_22 | 31975 | 33111 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| IEBOJFGD_21 | 4607 | 5476 | pdxS | 1.51e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| IEBOJFGD_12 | 342605 | 341829 | CodY | 4.70e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IEBOJFGD_26 | 133117 | 133509 | spxA1 | 7.40e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| IEBOJFGD_19 | 75207 | 75010 | CspA | 1.28e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| IEBOJFGD_34 | 23620 | 23814 | CspD | 5.44e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| IEBOJFGD_34 | 23620 | 23817 | CspB | 1.13e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| IEBOJFGD_19 | 75207 | 75013 | CspR | 1.68e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| IEBOJFGD_5 | 136911 | 136039 | lipA (SAUSA300_0829) | 4.44e-163 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IEBOJFGD_2 | 1.0 | 0.0 | 1.0 |
| IEBOJFGD_4 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_5 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_6 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_7 | 1.0 | 0.0 | 1.0 |
| IEBOJFGD_8 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_9 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_10 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_11 | 1.0 | 0.0 | 1.0 |
| IEBOJFGD_12 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_16 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_17 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_18 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_19 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_21 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_22 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_23 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_24 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_25 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_26 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_27 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_28 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_29 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_30 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_31 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_32 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_33 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_34 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_35 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_36 | 0.0 | 1.0 | 0.0 |
| IEBOJFGD_37 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IEBOJFGD_26 | 181422 | 191227 | False | Siphoviridae | VOG7718, VOG3390, VOG4705, VOG8610, VOG4995, VOG3699, VOG0221, VOG7734, VOG0720, VOG4555, VOG4572, VOG4713 |
| IEBOJFGD_26 | 253573 | 280370 | False | Myoviridae | VOG9667, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG4545, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG3390, VOG4705, VOG0753 |
| IEBOJFGD_34 | 40771 | 67608 | False | Siphoviridae | VOG3532, VOG10230, VOG0641, VOG7896, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581 |
| IEBOJFGD_34 | 173183 | 206380 | False | Myoviridae / Siphoviridae | VOG5722, VOG5779, VOG5721, VOG6031, VOG6524, VOG6246, VOG6941, VOG6036, VOG6472, VOG9219, VOG9713, VOG6940, VOG5534, VOG7718, VOG3390, VOG4705, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG4660 |
Phigaro v2.4.0 (Database: Jan. 2024 release)