Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 20 | 29 | 35.23 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NMKGIHNB_1 | 1055953 | 1057413 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.60 | 100.00 | WP_011681177 |
| NMKGIHNB_1 | 1055953 | 1057413 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.85 | 100.21 | WP_011226049 |
| NMKGIHNB_1 | 430519 | 431234 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 96.51 | 99.86 | WP_011681442 |
| NMKGIHNB_1 | 1055953 | 1057413 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.30 | 100.21 | WP_041827043 |
| NMKGIHNB_1 | 1052348 | 1053713 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.24 | 99.85 | WP_011681173 |
| NMKGIHNB_1 | 429549 | 430517 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 94.94 | 100.00 | WP_011681443 |
| NMKGIHNB_1 | 426359 | 427210 | rfbD | 0.0 | Capsule | Immune modulation | 94.84 | 100.00 | WP_014621820 |
| NMKGIHNB_1 | 1052346 | 1053713 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.37 | 100.00 | WP_011226045 |
| NMKGIHNB_1 | 1053770 | 1054510 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.20 | 100.00 | WP_011681174 |
| NMKGIHNB_1 | 1231348 | 1231941 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 93.60 | 100.00 | WP_011226149 |
| NMKGIHNB_1 | 1231348 | 1231941 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 93.43 | 100.00 | WP_011226149 |
| NMKGIHNB_1 | 1231348 | 1231941 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 93.43 | 100.00 | WP_011226149 |
| NMKGIHNB_1 | 1055221 | 1055952 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.31 | 100.00 | WP_011681176 |
| NMKGIHNB_1 | 1053770 | 1054510 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.25 | 100.00 | WP_011226046 |
| NMKGIHNB_1 | 1055221 | 1055952 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.17 | 100.00 | WP_011226048 |
| NMKGIHNB_1 | 1053775 | 1054510 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.94 | 98.13 | WP_011227246 |
| NMKGIHNB_1 | 431580 | 432356 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.79 | 100.00 | WP_011681440 |
| NMKGIHNB_1 | 1055221 | 1055952 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 92.49 | 100.00 | WP_011227248 |
| NMKGIHNB_1 | 427329 | 428582 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 92.34 | 99.44 | WP_011681445 |
| NMKGIHNB_1 | 1231941 | 1232810 | rfbA | 0.0 | Capsule | Immune modulation | 91.49 | 100.00 | WP_011681286 |
| NMKGIHNB_1 | 2187811 | 2188650 | cbpD | 0.0 | Autolysin | Exoenzyme | 90.24 | 100.00 | WP_011226733 |
| NMKGIHNB_1 | 942868 | 944526 | fbp54 | 0.0 | FBPs | Adherence | 89.76 | 99.94 | WP_011227179 |
| NMKGIHNB_1 | 1054520 | 1055212 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.47 | 99.86 | WP_011227247 |
| NMKGIHNB_1 | 1054520 | 1055212 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.47 | 99.86 | WP_011226047 |
| NMKGIHNB_1 | 104460 | 105470 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.45 | 99.80 | WP_002986042 |
| NMKGIHNB_1 | 1403085 | 1404388 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| NMKGIHNB_1 | 1054520 | 1055212 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.61 | 99.86 | WP_011681175 |
| NMKGIHNB_1 | 792382 | 793143 | srtA | 0.0 | Sortase A | Exoenzyme | 84.03 | 99.74 | - |
| NMKGIHNB_1 | 1230038 | 1231084 | rfbB | 0.0 | Capsule | Immune modulation | 83.89 | 99.81 | WP_002263087 |
| NMKGIHNB_1 | 2080334 | 2081617 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.76 | 99.61 | WP_000107753 |
| NMKGIHNB_1 | 1042622 | 1043205 | SPD_RS01775 | 2.92e-153 | Capsule | Immune modulation | 83.79 | 96.04 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NMKGIHNB_1 | 789693 | 788719 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.31 | 99.09 | AAK75326 |
| NMKGIHNB_1 | 801273 | 802346 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| NMKGIHNB_1 | 231292 | 228986 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.28 | 99.22 | AAK74619 |
| NMKGIHNB_1 | 659252 | 657771 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| NMKGIHNB_1 | 820333 | 822579 | metE | 0.0 | methionine biosynthesis enzymes | 93.06 | 100.00 | AAK74738 |
| NMKGIHNB_1 | 1497974 | 1497006 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| NMKGIHNB_1 | 2203699 | 2202728 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| NMKGIHNB_1 | 1259677 | 1257947 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| NMKGIHNB_1 | 384689 | 385840 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| NMKGIHNB_1 | 412924 | 414912 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| NMKGIHNB_1 | 2240652 | 2242130 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| NMKGIHNB_1 | 1404388 | 1403087 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| NMKGIHNB_1 | 2255966 | 2257858 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.26 | 98.90 | ABP91129 |
| NMKGIHNB_1 | 825500 | 823791 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| NMKGIHNB_1 | 2167916 | 2166825 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| NMKGIHNB_1 | 56428 | 55418 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| NMKGIHNB_1 | 960030 | 958669 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| NMKGIHNB_1 | 1418796 | 1417906 | bglB | 2.89e-177 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| NMKGIHNB_1 | 866288 | 865338 | SP_0676 | 3.70e-169 | transcription facot | 81.39 | 98.45 | AAK74821 |
| NMKGIHNB_1 | 822769 | 823626 | metF | 1.37e-168 | methionine biosynthesis enzymes | 88.11 | 99.31 | AAK74739 |
| NMKGIHNB_1 | 713375 | 714046 | rr01 | 7.26e-126 | response regulator | 84.82 | 99.56 | CAB54566 |
| NMKGIHNB_1 | 1103186 | 1102482 | covR | 3.67e-125 | responder protein | 85.11 | 100.00 | AAK71319 |
| NMKGIHNB_1 | 829467 | 830138 | ciaR | 2.22e-124 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| NMKGIHNB_1 | 829467 | 830135 | CiaR | 4.11e-124 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| NMKGIHNB_1 | 1938634 | 1937948 | CovR | 5.07e-119 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| NMKGIHNB_1 | 1103186 | 1102482 | walR | 6.74e-119 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| NMKGIHNB_1 | 2166650 | 2166255 | SpxA2 | 2.28e-74 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| NMKGIHNB_1 | 971496 | 971897 | SpxA1 | 4.75e-69 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| NMKGIHNB_1 | 2096424 | 2096011 | GrpE | 4.27e-66 | protein grpE | 81.16 | 80.59 | ABP89267 |
| NMKGIHNB_1 | 2080167 | 2079676 | RopE (M5005_Spy_1611) | 1.12e-71 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| NMKGIHNB_1 | 231292 | 228992 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.97 | 98.08 | MYN69560 |
| NMKGIHNB_1 | 19717 | 20736 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NMKGIHNB_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)