Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IHJEBJDG_12 | 446408 | 446746 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IHJEBJDG_12 | 446091 | 446408 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IHJEBJDG_23 | 377850 | 379019 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| IHJEBJDG_53 | 192968 | 193561 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| IHJEBJDG_23 | 151510 | 152712 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| IHJEBJDG_23 | 134765 | 135619 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| IHJEBJDG_4 | 45293 | 46732 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| IHJEBJDG_4 | 32478 | 33398 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| IHJEBJDG_3 | 28708 | 30351 | vmlR | ARO:3004476 | vmlR | 99.09 | 100.00 | NC_000964.3 |
| IHJEBJDG_45 | 27191 | 29761 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IHJEBJDG_23 | 134765 | 135619 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| IHJEBJDG_4 | 32478 | 33398 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IHJEBJDG_3 | 28708 | 30348 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.09 | 100.00 | WP_003234144.1 |
| IHJEBJDG_23 | 134765 | 135616 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| IHJEBJDG_4 | 32478 | 33395 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| IHJEBJDG_1 | 39590 | 40108 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.80 | 100.00 | WP_003242546.1 |
| IHJEBJDG_52 | 54026 | 56623 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.41 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IHJEBJDG_52 | 167904 | 168545 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| IHJEBJDG_1 | 531351 | 532532 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IHJEBJDG_34 | 300238 | 301023 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| IHJEBJDG_34 | 301049 | 302245 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| IHJEBJDG_52 | 8256 | 10019 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| IHJEBJDG_1 | 533015 | 534157 | capA | 0.0 | Capsule | Immune modulation | 98.69 | 100.00 | NP_391469 |
| IHJEBJDG_1 | 532547 | 532996 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| IHJEBJDG_34 | 303921 | 304859 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| IHJEBJDG_34 | 304879 | 312015 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.36 | 100.00 | NP_391076 |
| IHJEBJDG_34 | 302274 | 303893 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IHJEBJDG_8 | 31640 | 32776 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IHJEBJDG_13 | 11112 | 10243 | pdxS | 1.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IHJEBJDG_12 | 137639 | 136863 | CodY | 3.95e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IHJEBJDG_34 | 36198 | 35629 | ClpP | 2.49e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| IHJEBJDG_51 | 275528 | 275920 | spxA1 | 2.03e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| IHJEBJDG_51 | 275528 | 275920 | spx | 1.21e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| IHJEBJDG_9 | 20482 | 20724 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| IHJEBJDG_3 | 74144 | 73947 | CspA | 3.86e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| IHJEBJDG_52 | 179777 | 179971 | CspD | 8.06e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| IHJEBJDG_52 | 179777 | 179974 | CspB | 1.67e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IHJEBJDG_1 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_2 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_3 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_4 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_5 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_6 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_7 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_8 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_9 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_10 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_11 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_12 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_13 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_14 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_15 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_16 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_17 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_19 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_20 | 1.0 | 0.0 | 1.0 |
| IHJEBJDG_21 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_23 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_24 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_25 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_26 | 1.0 | 0.0 | 1.0 |
| IHJEBJDG_27 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_34 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_45 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_51 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_52 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_53 | 0.0 | 1.0 | 0.0 |
| IHJEBJDG_54 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IHJEBJDG_2 | 192 | 17334 | False | Siphoviridae | VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841 |
| IHJEBJDG_5 | 6438 | 14641 | False | Unknown | VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
| IHJEBJDG_12 | 477445 | 483609 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894 |
| IHJEBJDG_23 | 188571 | 206339 | False | Myoviridae | VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG0906, VOG7718 |
| IHJEBJDG_45 | 283411 | 306841 | False | Myoviridae | VOG4574, VOG0136, VOG0753, VOG5008, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720 |
| IHJEBJDG_45 | 319377 | 319931 | False | Unknown | VOG0650 |
| IHJEBJDG_54 | 151 | 27969 | False | Siphoviridae | VOG4619, VOG0801, VOG4545, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG1886, VOG4581, VOG4556, VOG4841, VOG0198, VOG4548, VOG0327, VOG4609, VOG0026, VOG1565, VOG0021, VOG1047, VOG7438 |
Phigaro v2.4.0 (Database: Jan. 2024 release)