Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HBJADMOB_12 | 624063 | 625232 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| HBJADMOB_12 | 453920 | 455122 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| HBJADMOB_12 | 435523 | 436377 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| HBJADMOB_22 | 188814 | 190460 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| HBJADMOB_23 | 137260 | 137853 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| HBJADMOB_23 | 86735 | 88174 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| HBJADMOB_23 | 73921 | 74841 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
| HBJADMOB_19 | 42708 | 43025 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| HBJADMOB_19 | 42370 | 42708 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| HBJADMOB_21 | 27194 | 29764 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HBJADMOB_1 | 36175 | 37551 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.93 | 100.00 | X08034 |
| HBJADMOB_23 | 73921 | 74841 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| HBJADMOB_12 | 435523 | 436375 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HBJADMOB_1 | 36175 | 37548 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| HBJADMOB_23 | 73921 | 74838 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| HBJADMOB_22 | 188817 | 190460 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| HBJADMOB_12 | 435523 | 436374 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| HBJADMOB_1 | 39504 | 40022 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| HBJADMOB_12 | 929833 | 932430 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HBJADMOB_1 | 508407 | 509588 | capB | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | NP_391471 |
| HBJADMOB_12 | 823974 | 824615 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| HBJADMOB_1 | 930683 | 931468 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| HBJADMOB_1 | 934366 | 935304 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| HBJADMOB_1 | 935324 | 942460 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.31 | 100.00 | NP_391076 |
| HBJADMOB_1 | 510071 | 511213 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| HBJADMOB_1 | 509603 | 510052 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| HBJADMOB_1 | 931494 | 932690 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| HBJADMOB_12 | 977504 | 979267 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| HBJADMOB_1 | 932719 | 934338 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.89 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HBJADMOB_2 | 31673 | 32809 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| HBJADMOB_5 | 4229 | 5098 | pdxS | 1.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| HBJADMOB_19 | 352754 | 353530 | CodY | 4.44e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HBJADMOB_1 | 663541 | 662972 | ClpP | 6.28e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| HBJADMOB_20 | 274732 | 275124 | spxA1 | 2.45e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| HBJADMOB_20 | 274732 | 275124 | spx | 1.46e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| HBJADMOB_3 | 20477 | 20719 | SpoVG | 7.47e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| HBJADMOB_22 | 141995 | 142192 | CspA | 9.47e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| HBJADMOB_20 | 31511 | 31317 | CspB | 3.53e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HBJADMOB_1 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_2 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_3 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_4 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_5 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_6 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_7 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_8 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_9 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_10 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_12 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_14 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_15 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_19 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_20 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_21 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_22 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_23 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_24 | 0.0 | 1.0 | 0.0 |
| HBJADMOB_25 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HBJADMOB_19 | 4279 | 10494 | False | Myoviridae | VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| HBJADMOB_20 | 368895 | 378816 | False | Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685 |
| HBJADMOB_24 | 27144 | 36680 | False | Myoviridae / Siphoviridae | VOG4724, VOG1198, VOG0918, VOG4551, VOG0025 |
Phigaro v2.4.0 (Database: Jan. 2024 release)