Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 16 | 32 | 35.78 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OJFKPEJI_22 | 68231 | 68971 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.87 | 100.00 | WP_011681174 |
| OJFKPEJI_22 | 68231 | 68971 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 94.20 | 100.00 | WP_011226046 |
| OJFKPEJI_22 | 65328 | 66788 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 93.98 | 100.14 | WP_011226049 |
| OJFKPEJI_22 | 65328 | 66788 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.98 | 100.00 | WP_011681177 |
| OJFKPEJI_1 | 93475 | 94326 | rfbD | 0.0 | Capsule | Immune modulation | 93.90 | 100.00 | WP_014621820 |
| OJFKPEJI_22 | 69028 | 70395 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.71 | 100.00 | WP_011226045 |
| OJFKPEJI_22 | 68231 | 68966 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.61 | 98.13 | WP_011227246 |
| OJFKPEJI_22 | 69028 | 70393 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.56 | 99.85 | WP_011681173 |
| OJFKPEJI_22 | 65328 | 66788 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.22 | 100.14 | WP_041827043 |
| OJFKPEJI_22 | 66789 | 67520 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.26 | 100.00 | WP_011226048 |
| OJFKPEJI_12 | 239290 | 239883 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| OJFKPEJI_22 | 66789 | 67520 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011681176 |
| OJFKPEJI_12 | 239290 | 239883 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| OJFKPEJI_12 | 239290 | 239883 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| OJFKPEJI_12 | 238421 | 239290 | rfbA | 0.0 | Capsule | Immune modulation | 90.58 | 100.00 | WP_011681286 |
| OJFKPEJI_22 | 172843 | 174501 | fbp54 | 0.0 | FBPs | Adherence | 90.49 | 99.88 | WP_011227179 |
| OJFKPEJI_22 | 66789 | 67520 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.44 | 100.00 | WP_011227248 |
| OJFKPEJI_27 | 91803 | 92708 | cbpD | 0.0 | Autolysin | Exoenzyme | 89.51 | 93.50 | WP_082302295 |
| OJFKPEJI_1 | 390640 | 391650 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.45 | 99.80 | WP_002986042 |
| OJFKPEJI_22 | 67529 | 68221 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.33 | 99.86 | WP_011227247 |
| OJFKPEJI_22 | 67529 | 68221 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.33 | 99.86 | WP_011226047 |
| OJFKPEJI_12 | 69275 | 70578 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| OJFKPEJI_22 | 67529 | 68221 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.18 | 99.86 | WP_011681175 |
| OJFKPEJI_25 | 21818 | 22579 | srtA | 0.0 | Sortase A | Exoenzyme | 84.29 | 99.74 | - |
| OJFKPEJI_12 | 240086 | 241132 | rfbB | 0.0 | Capsule | Immune modulation | 84.16 | 99.90 | WP_002263087 |
| OJFKPEJI_24 | 190179 | 191462 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.61 | 99.61 | WP_000107753 |
| OJFKPEJI_22 | 79534 | 80119 | SPD_RS01775 | 3.05e-145 | Capsule | Immune modulation | 82.79 | 96.53 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OJFKPEJI_25 | 13687 | 12614 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| OJFKPEJI_25 | 25268 | 26242 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 95.69 | 99.09 | AAK75326 |
| OJFKPEJI_1 | 270858 | 273164 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| OJFKPEJI_28 | 49238 | 47757 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| OJFKPEJI_26 | 18824 | 17856 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| OJFKPEJI_22 | 302791 | 300545 | metE | 0.0 | methionine biosynthesis enzymes | 92.26 | 100.00 | AAK74738 |
| OJFKPEJI_4 | 38819 | 37848 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| OJFKPEJI_12 | 213034 | 214764 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 90.12 | 100.00 | AAK34199 |
| OJFKPEJI_1 | 136387 | 135236 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| OJFKPEJI_1 | 107854 | 105866 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.29 | 100.00 | AE014259_2 |
| OJFKPEJI_23 | 184902 | 183424 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| OJFKPEJI_12 | 69275 | 70576 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| OJFKPEJI_23 | 169583 | 167691 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.42 | 98.90 | ABP91129 |
| OJFKPEJI_22 | 297626 | 299335 | PGM | 0.0 | phosphoglucomutase | 85.11 | 99.65 | ACH87041 |
| OJFKPEJI_4 | 1875 | 784 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| OJFKPEJI_23 | 28667 | 29677 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| OJFKPEJI_22 | 157292 | 158653 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.89 | 99.56 | ABP90420 |
| OJFKPEJI_12 | 54866 | 55756 | bglB | 3.46e-177 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| OJFKPEJI_22 | 256921 | 257871 | SP_0676 | 4.37e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| OJFKPEJI_22 | 300358 | 299501 | metF | 7.71e-170 | methionine biosynthesis enzymes | 88.11 | 99.31 | AAK74739 |
| OJFKPEJI_25 | 92250 | 91579 | rr01 | 2.42e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| OJFKPEJI_22 | 19654 | 20358 | covR | 1.37e-126 | responder protein | 85.53 | 100.00 | AAK71319 |
| OJFKPEJI_22 | 293732 | 293061 | ciaR | 2.95e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| OJFKPEJI_22 | 293732 | 293064 | CiaR | 5.46e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| OJFKPEJI_22 | 19654 | 20358 | walR | 3.12e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| OJFKPEJI_24 | 53669 | 52983 | CovR | 4.22e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| OJFKPEJI_4 | 611 | 216 | SpxA2 | 4.24e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| OJFKPEJI_22 | 145825 | 145424 | SpxA1 | 3.41e-69 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| OJFKPEJI_3 | 6826 | 6413 | GrpE | 1.25e-66 | protein grpE | 81.16 | 80.59 | ABP89267 |
| OJFKPEJI_24 | 30457 | 30170 | spr0479 | 5.11e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| OJFKPEJI_26 | 51899 | 51645 | spr1327 | 7.91e-45 | essential gene | 91.77 | 100.00 | AAL00131 |
| OJFKPEJI_23 | 117920 | 118183 | spr0175 | 1.09e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| OJFKPEJI_23 | 118744 | 119010 | spr0177 | 4.01e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| OJFKPEJI_1 | 270858 | 273158 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.36 | 98.08 | MYN69560 |
| OJFKPEJI_23 | 65081 | 64062 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
| OJFKPEJI_24 | 190009 | 189614 | RopE (M5005_Spy_1611) | 5.08e-69 | probable DNA-directed RNA polymerase subunit delta | 86.36 | 69.11 | AAK34603 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OJFKPEJI_1 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_2 | 1.0 | 0.0 | 1.0 |
| OJFKPEJI_3 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_4 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_5 | 1.0 | 0.0 | 1.0 |
| OJFKPEJI_6 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_7 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_8 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_9 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_10 | 1.0 | 0.0 | 1.0 |
| OJFKPEJI_12 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_14 | 1.0 | 0.0 | 1.0 |
| OJFKPEJI_15 | 1.0 | 0.0 | 1.0 |
| OJFKPEJI_16 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_17 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_18 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_19 | 1.0 | 0.0 | 1.0 |
| OJFKPEJI_21 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_22 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_23 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_24 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_25 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_26 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_27 | 0.0 | 1.0 | 0.0 |
| OJFKPEJI_28 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OJFKPEJI_23 | 155046 | 160111 | False | Siphoviridae | VOG4606, VOG4632, VOG8336, VOG3866, VOG4603 |
Phigaro v2.4.0 (Database: Jan. 2024 release)