safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	28	37.54

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
KDLGDBLP_1	1356872	1357681	STU_RS16245	Capsule	Immune modulation	99.88	100.00	WP_002948020
KDLGDBLP_1	1357665	1358621	STU_RS16250	Capsule	Immune modulation	99.79	100.00	WP_011226328
KDLGDBLP_1	1358618	1359766	STU_RS16255	Capsule	Immune modulation	99.74	100.00	WP_041828290
KDLGDBLP_1	1363262	1364113	rfbD	Capsule	Immune modulation	99.65	100.00	WP_014621820
KDLGDBLP_1	872842	874500	fbp54	FBPs	Adherence	99.64	100.00	WP_011227179
KDLGDBLP_1	1355667	1356872	STU_RS16240	Capsule	Immune modulation	99.58	100.00	WP_011226326
KDLGDBLP_1	43615	44454	cbpD	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
KDLGDBLP_1	1359875	1360885	STU_RS16260	Capsule	Immune modulation	99.50	100.00	WP_011226330
KDLGDBLP_1	1354592	1355647	STU_RS16235	Capsule	Immune modulation	99.43	100.00	WP_002948022
KDLGDBLP_1	1118583	1119452	rfbA	Capsule	Immune modulation	99.42	100.00	WP_011681286
KDLGDBLP_1	1352811	1354595	STU_RS16230	Capsule	Immune modulation	99.38	100.00	WP_011226325
KDLGDBLP_1	1117990	1118583	STR_RS05880	Capsule	Immune modulation	99.33	100.00	WP_011226149
KDLGDBLP_1	1117990	1118583	STER_RS06030	Capsule	Immune modulation	99.16	100.00	WP_011226149
KDLGDBLP_1	1117990	1118583	STU_RS15260	Capsule	Immune modulation	99.16	100.00	WP_011226149
KDLGDBLP_1	992976	993671	STU_RS14640	Capsule	Immune modulation	98.99	100.29	WP_011226047
KDLGDBLP_1	992976	993671	STR_RS05255	Capsule	Immune modulation	98.42	100.29	WP_011227247
KDLGDBLP_1	1060353	1061146	cshA	Cell surface hydrophobicity proteins	Adherence	98.24	100.25	-
KDLGDBLP_1	993680	994411	STU_RS14645	Capsule	Immune modulation	98.22	100.00	WP_011226048
KDLGDBLP_1	994412	995872	STER_RS05320	Capsule	Immune modulation	98.02	100.00	WP_011681177
KDLGDBLP_1	993680	994411	STER_RS05315	Capsule	Immune modulation	97.95	100.00	WP_011681176
KDLGDBLP_1	983653	985068	STER_RS05250	Capsule	Immune modulation	97.88	100.00	WP_011681165
KDLGDBLP_1	1154790	1155551	srtA	Sortase A	Exoenzyme	97.64	100.00	-
KDLGDBLP_1	993680	994411	STR_RS05260	Capsule	Immune modulation	97.54	100.00	WP_011227248
KDLGDBLP_1	1360888	1362159	STU_RS16265	Capsule	Immune modulation	97.48	99.76	WP_011226331
KDLGDBLP_1	994412	995872	STU_RS14650	Capsule	Immune modulation	97.26	100.21	WP_011226049
KDLGDBLP_1	994412	995872	STR_RS05265	Capsule	Immune modulation	96.30	100.21	WP_041827043
KDLGDBLP_1	992208	992966	STER_RS05305	Capsule	Immune modulation	95.52	100.94	WP_011681174
KDLGDBLP_1	992208	992966	STU_RS14635	Capsule	Immune modulation	95.39	100.94	WP_011226046
KDLGDBLP_1	990786	992151	STER_RS05300	Capsule	Immune modulation	95.24	99.85	WP_011681173
KDLGDBLP_1	992213	992966	STR_RS05250	Capsule	Immune modulation	94.56	99.07	WP_011227246
KDLGDBLP_1	990784	992151	STU_RS14630	Capsule	Immune modulation	94.01	100.00	WP_011226045
KDLGDBLP_1	992976	993671	STER_RS05310	Capsule	Immune modulation	88.94	100.29	WP_011681175
KDLGDBLP_1	620509	621812	eno	Streptococcal enolase	Exoenzyme	88.17	99.54	WP_000022832
KDLGDBLP_1	1653926	1654936	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.44	99.41	WP_002986042
KDLGDBLP_1	152258	153541	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
KDLGDBLP_1	1116514	1117560	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
KDLGDBLP_1	1158238	1159221	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
KDLGDBLP_1	726623	727696	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
KDLGDBLP_1	536824	537792	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
KDLGDBLP_1	1530715	1533018	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
KDLGDBLP_1	1253050	1254531	SPD_0310	0.0	UPF0371 protein SPD_0310	92.31	100.00	ABJ54480
KDLGDBLP_1	748565	750811	metE	0.0	methionine biosynthesis enzymes	91.72	100.00	AAK74738
KDLGDBLP_1	72227	73225	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
KDLGDBLP_1	27132	28103	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
KDLGDBLP_1	1415751	1414600	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
KDLGDBLP_1	1379083	1377095	uvrB	0.0	excinuclease ABC, B subunit	88.99	100.00	AE014259_2
KDLGDBLP_1	1142030	1140300	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
KDLGDBLP_1	1874908	1873430	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
KDLGDBLP_1	620509	621810	Eno	0.0	enolase	86.21	99.77	ABP90468
KDLGDBLP_1	1859719	1857827	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
KDLGDBLP_1	753736	752027	PGM	0.0	phosphoglucomutase	83.71	99.65	ACH87041
KDLGDBLP_1	1698395	1699405	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
KDLGDBLP_1	907652	906291	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
KDLGDBLP_1	1750439	1748547	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
KDLGDBLP_1	606074	606964	bglB	1.35e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
KDLGDBLP_1	814806	813868	SP_0676	2.40e-167	transcription facot	81.15	97.20	AAK74821
KDLGDBLP_1	751003	751860	metF	1.56e-164	methionine biosynthesis enzymes	86.36	99.31	AAK74739
KDLGDBLP_1	1032673	1031969	covR	4.46e-131	responder protein	85.53	100.00	AAK71319
KDLGDBLP_1	1211858	1211187	rr01	3.06e-124	response regulator	83.93	99.56	CAB54566
KDLGDBLP_1	1032673	1031969	walR	1.88e-121	DNA-binding response regulator	82.13	100.00	ANC99504
KDLGDBLP_1	310296	310982	CovR	1.02e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
KDLGDBLP_1	757690	758358	CiaR	2.06e-117	two-component response regulator	84.31	99.55	AAK74935
KDLGDBLP_1	757690	758361	ciaR	2.02e-115	two component system response regulator	82.14	99.12	AIK75708
KDLGDBLP_1	919012	919413	SpxA1	4.88e-66	transcriptional regulator	80.60	100.00	ERL19273
KDLGDBLP_1	153699	154199	RopE (M5005_Spy_1611)	5.01e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
KDLGDBLP_1	1530715	1533012	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
KDLGDBLP_1	1735967	1734948	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
KDLGDBLP_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found