Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 33 | 41.40 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AFNEFDJD_3 | 19511 | 20380 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| AFNEFDJD_3 | 20380 | 20973 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| AFNEFDJD_3 | 20380 | 20973 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| AFNEFDJD_30 | 187316 | 188272 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| AFNEFDJD_30 | 188256 | 189065 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_002948020 |
| AFNEFDJD_30 | 186171 | 187319 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| AFNEFDJD_3 | 20380 | 20973 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| AFNEFDJD_30 | 189065 | 190270 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226326 |
| AFNEFDJD_30 | 191342 | 193126 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.55 | 100.00 | WP_011226325 |
| AFNEFDJD_30 | 181824 | 182675 | rfbD | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_014621820 |
| AFNEFDJD_4 | 222156 | 223814 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| AFNEFDJD_30 | 185052 | 186062 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226330 |
| AFNEFDJD_30 | 190290 | 191345 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.43 | 100.00 | WP_002948022 |
| AFNEFDJD_4 | 110878 | 111609 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | WP_011681176 |
| AFNEFDJD_4 | 110878 | 111609 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011226048 |
| AFNEFDJD_4 | 110878 | 111609 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011227248 |
| AFNEFDJD_4 | 44234 | 45022 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.36 | 99.75 | - |
| AFNEFDJD_10 | 16103 | 17062 | cbpD | 0.0 | Autolysin | Exoenzyme | 97.83 | 99.90 | WP_082302295 |
| AFNEFDJD_4 | 120135 | 121550 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011681165 |
| AFNEFDJD_2 | 25669 | 26430 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| AFNEFDJD_30 | 183778 | 185049 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.48 | 99.76 | WP_011226331 |
| AFNEFDJD_4 | 109417 | 110877 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.47 | 100.00 | WP_011681177 |
| AFNEFDJD_4 | 109417 | 110877 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.85 | 100.21 | WP_011226049 |
| AFNEFDJD_4 | 109417 | 110877 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.89 | 100.21 | WP_041827043 |
| AFNEFDJD_4 | 111618 | 112310 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011227247 |
| AFNEFDJD_4 | 111618 | 112310 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011226047 |
| AFNEFDJD_4 | 113135 | 114500 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.17 | 99.85 | WP_011681173 |
| AFNEFDJD_4 | 112320 | 113078 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 93.94 | 100.94 | WP_011681174 |
| AFNEFDJD_4 | 113135 | 114502 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.93 | 100.00 | WP_011226045 |
| AFNEFDJD_4 | 112320 | 113078 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.81 | 100.94 | WP_011226046 |
| AFNEFDJD_4 | 112320 | 113073 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.24 | 99.07 | WP_011227246 |
| AFNEFDJD_4 | 111618 | 112310 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| AFNEFDJD_9 | 116127 | 117430 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| AFNEFDJD_27 | 14363 | 15373 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.45 | 99.90 | WP_002986042 |
| AFNEFDJD_15 | 78548 | 79831 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| AFNEFDJD_3 | 21402 | 22448 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AFNEFDJD_2 | 22982 | 21999 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| AFNEFDJD_9 | 13907 | 12834 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| AFNEFDJD_9 | 200829 | 199861 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| AFNEFDJD_30 | 15603 | 13300 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| AFNEFDJD_30 | 288326 | 286845 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| AFNEFDJD_8 | 11368 | 9122 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| AFNEFDJD_18 | 2088 | 1096 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| AFNEFDJD_19 | 34394 | 33423 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| AFNEFDJD_30 | 129389 | 130540 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| AFNEFDJD_30 | 166533 | 168521 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| AFNEFDJD_2 | 39178 | 40908 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| AFNEFDJD_1 | 27027 | 28505 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| AFNEFDJD_9 | 117430 | 116129 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| AFNEFDJD_1 | 42217 | 44109 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| AFNEFDJD_8 | 6197 | 7906 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| AFNEFDJD_23 | 14486 | 13476 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| AFNEFDJD_4 | 197863 | 199224 | mnmE | 0.0 | central tRNA-modifying GTPase | 81.80 | 99.56 | ABP90420 |
| AFNEFDJD_12 | 22159 | 24051 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| AFNEFDJD_9 | 131865 | 130975 | bglB | 1.87e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| AFNEFDJD_5 | 10468 | 11406 | SP_0676 | 3.54e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| AFNEFDJD_8 | 8930 | 8073 | metF | 1.50e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| AFNEFDJD_4 | 72709 | 73413 | covR | 6.40e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| AFNEFDJD_30 | 307303 | 307974 | rr01 | 5.11e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| AFNEFDJD_4 | 72709 | 73413 | walR | 2.70e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| AFNEFDJD_8 | 2243 | 1575 | CiaR | 1.64e-119 | two-component response regulator | 84.75 | 99.55 | AAK74935 |
| AFNEFDJD_14 | 7854 | 7168 | CovR | 4.40e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| AFNEFDJD_8 | 2243 | 1572 | ciaR | 1.78e-117 | two component system response regulator | 82.59 | 99.12 | AIK75708 |
| AFNEFDJD_18 | 784 | 389 | SpxA2 | 8.17e-76 | transcriptional regulator | 90.15 | 100.00 | ERL21461 |
| AFNEFDJD_4 | 186504 | 186103 | SpxA1 | 5.51e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| AFNEFDJD_11 | 25699 | 25412 | spr0479 | 2.97e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| AFNEFDJD_9 | 235240 | 234986 | spr1327 | 3.88e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| AFNEFDJD_1 | 84818 | 84555 | spr0175 | 4.51e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| AFNEFDJD_1 | 83991 | 83725 | spr0177 | 9.87e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| AFNEFDJD_15 | 78390 | 77890 | RopE (M5005_Spy_1611) | 2.36e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| AFNEFDJD_30 | 15603 | 13306 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| AFNEFDJD_12 | 36568 | 37587 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 95.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AFNEFDJD_1 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_2 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_3 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_4 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_5 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_6 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_7 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_8 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_9 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_10 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_11 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_12 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_13 | 1.0 | 0.0 | 1.0 |
| AFNEFDJD_14 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_15 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_16 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_17 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_18 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_19 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_20 | 1.0 | 0.0 | 1.0 |
| AFNEFDJD_21 | 1.0 | 0.0 | 1.0 |
| AFNEFDJD_22 | 1.0 | 0.0 | 1.0 |
| AFNEFDJD_23 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_24 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_25 | 1.0 | 0.0 | 1.0 |
| AFNEFDJD_26 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_27 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_28 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_29 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_30 | 0.0 | 1.0 | 0.0 |
| AFNEFDJD_31 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)