safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	28	37.54

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
OEFGDGPG_1	1137607	1138476	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
OEFGDGPG_1	1137014	1137607	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
OEFGDGPG_1	1137014	1137607	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
OEFGDGPG_1	1351625	1352581	STU_RS16250	Capsule	Immune modulation	99.79	100.00	WP_011226328
OEFGDGPG_1	1350832	1351641	STU_RS16245	Capsule	Immune modulation	99.75	100.00	WP_002948020
OEFGDGPG_1	1352578	1353726	STU_RS16255	Capsule	Immune modulation	99.74	100.00	WP_041828290
OEFGDGPG_1	1137014	1137607	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
OEFGDGPG_1	1349627	1350832	STU_RS16240	Capsule	Immune modulation	99.58	100.00	WP_011226326
OEFGDGPG_1	1346771	1348555	STU_RS16230	Capsule	Immune modulation	99.55	100.00	WP_011226325
OEFGDGPG_1	1357222	1358073	rfbD	Capsule	Immune modulation	99.53	100.00	WP_014621820
OEFGDGPG_1	43686	44525	cbpD	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
OEFGDGPG_1	1353835	1354845	STU_RS16260	Capsule	Immune modulation	99.50	100.00	WP_011226330
OEFGDGPG_1	1348552	1349607	STU_RS16235	Capsule	Immune modulation	99.43	100.00	WP_002948022
OEFGDGPG_1	901788	903446	fbp54	FBPs	Adherence	99.34	100.00	WP_011227179
OEFGDGPG_1	1014076	1014807	STER_RS05315	Capsule	Immune modulation	99.04	100.00	WP_011681176
OEFGDGPG_1	1014076	1014807	STU_RS14645	Capsule	Immune modulation	98.77	100.00	WP_011226048
OEFGDGPG_1	1014076	1014807	STR_RS05260	Capsule	Immune modulation	98.63	100.00	WP_011227248
OEFGDGPG_1	1080814	1081602	cshA	Cell surface hydrophobicity proteins	Adherence	98.36	99.75	-
OEFGDGPG_1	1004135	1005550	STER_RS05250	Capsule	Immune modulation	97.81	100.00	WP_011681165
OEFGDGPG_1	1173798	1174559	srtA	Sortase A	Exoenzyme	97.64	100.00	-
OEFGDGPG_1	1354848	1356119	STU_RS16265	Capsule	Immune modulation	97.48	99.76	WP_011226331
OEFGDGPG_1	1014808	1016268	STER_RS05320	Capsule	Immune modulation	97.47	100.00	WP_011681177
OEFGDGPG_1	1014808	1016268	STU_RS14650	Capsule	Immune modulation	96.85	100.21	WP_011226049
OEFGDGPG_1	1014808	1016268	STR_RS05265	Capsule	Immune modulation	95.89	100.21	WP_041827043
OEFGDGPG_1	1013375	1014067	STR_RS05255	Capsule	Immune modulation	95.40	99.86	WP_011227247
OEFGDGPG_1	1013375	1014067	STU_RS14640	Capsule	Immune modulation	95.40	99.86	WP_011226047
OEFGDGPG_1	1011185	1012550	STER_RS05300	Capsule	Immune modulation	95.17	99.85	WP_011681173
OEFGDGPG_1	1012607	1013365	STER_RS05305	Capsule	Immune modulation	93.94	100.94	WP_011681174
OEFGDGPG_1	1011183	1012550	STU_RS14630	Capsule	Immune modulation	93.93	100.00	WP_011226045
OEFGDGPG_1	1012607	1013365	STU_RS14635	Capsule	Immune modulation	93.81	100.94	WP_011226046
OEFGDGPG_1	1012612	1013365	STR_RS05250	Capsule	Immune modulation	93.24	99.07	WP_011227246
OEFGDGPG_1	1013375	1014067	STER_RS05310	Capsule	Immune modulation	89.47	100.00	WP_011681175
OEFGDGPG_1	638417	639720	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
OEFGDGPG_1	1646050	1647060	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	87.45	99.90	WP_002986042
OEFGDGPG_1	1135539	1136585	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423
OEFGDGPG_1	160784	162067	tig/ropA	Trigger factor	Stress survival	80.58	99.77	WP_000107753

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
OEFGDGPG_1	1177246	1178229	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
OEFGDGPG_1	742133	743206	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
OEFGDGPG_1	555019	555987	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
OEFGDGPG_1	1523042	1525345	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
OEFGDGPG_1	1251842	1253323	SPD_0310	0.0	UPF0371 protein SPD_0310	92.31	100.00	ABJ54480
OEFGDGPG_1	778576	780822	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
OEFGDGPG_1	65875	66867	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
OEFGDGPG_1	27202	28173	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
OEFGDGPG_1	1409257	1408106	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
OEFGDGPG_1	1373365	1371377	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
OEFGDGPG_1	1161050	1159320	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
OEFGDGPG_1	1854035	1852557	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
OEFGDGPG_1	638417	639718	Eno	0.0	enolase	86.21	99.77	ABP90468
OEFGDGPG_1	1838845	1836953	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
OEFGDGPG_1	783747	782038	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
OEFGDGPG_1	1689714	1690724	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
OEFGDGPG_1	927821	926460	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
OEFGDGPG_1	1741408	1739516	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
OEFGDGPG_1	623982	624872	bglB	5.33e-177	putative phospho--glucosidase	88.22	99.66	AAK33487
OEFGDGPG_1	843113	842175	SP_0676	2.37e-167	transcription facot	81.15	97.20	AAK74821
OEFGDGPG_1	781014	781871	metF	8.70e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
OEFGDGPG_1	1052976	1052272	covR	4.41e-131	responder protein	85.53	100.00	AAK71319
OEFGDGPG_1	1232866	1232195	rr01	5.10e-124	response regulator	83.93	99.56	CAB54566
OEFGDGPG_1	1052976	1052272	walR	1.86e-121	DNA-binding response regulator	82.13	100.00	ANC99504
OEFGDGPG_1	787701	788369	CiaR	9.47e-118	two-component response regulator	84.75	99.55	AAK74935
OEFGDGPG_1	315177	315863	CovR	1.01e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
OEFGDGPG_1	787701	788372	ciaR	1.03e-115	two component system response regulator	82.59	99.12	AIK75708
OEFGDGPG_1	67179	67574	SpxA2	3.20e-73	transcriptional regulator	90.15	100.00	ERL21461
OEFGDGPG_1	162225	162725	RopE (M5005_Spy_1611)	4.95e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
OEFGDGPG_1	1523042	1525339	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
OEFGDGPG_1	1726999	1725980	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	95.88	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
OEFGDGPG_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found