Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 33 | 41.40 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ICNKMEIA_5 | 39664 | 40473 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| ICNKMEIA_5 | 38724 | 39680 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| ICNKMEIA_5 | 37579 | 38727 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| ICNKMEIA_5 | 33232 | 34083 | rfbD | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_014621820 |
| ICNKMEIA_28 | 1729 | 3387 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| ICNKMEIA_5 | 40473 | 41678 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226326 |
| ICNKMEIA_5 | 36460 | 37470 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226330 |
| ICNKMEIA_5 | 41698 | 42753 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.43 | 100.00 | WP_002948022 |
| ICNKMEIA_17 | 19511 | 20380 | rfbA | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011681286 |
| ICNKMEIA_5 | 42750 | 44534 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | WP_011226325 |
| ICNKMEIA_17 | 20380 | 20973 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_011226149 |
| ICNKMEIA_17 | 20380 | 20973 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226149 |
| ICNKMEIA_17 | 20380 | 20973 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226149 |
| ICNKMEIA_20 | 66061 | 66756 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 99.14 | 100.29 | WP_011226047 |
| ICNKMEIA_20 | 66061 | 66756 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 98.56 | 100.29 | WP_011227247 |
| ICNKMEIA_41 | 236408 | 237376 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.35 | 99.90 | WP_082302295 |
| ICNKMEIA_19 | 992 | 1785 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.24 | 100.25 | - |
| ICNKMEIA_20 | 65321 | 66052 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011226048 |
| ICNKMEIA_20 | 63860 | 65320 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.02 | 100.00 | WP_011681177 |
| ICNKMEIA_20 | 65321 | 66052 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.95 | 100.00 | WP_011681176 |
| ICNKMEIA_21 | 999 | 2414 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 97.88 | 100.00 | WP_011681165 |
| ICNKMEIA_11 | 30295 | 31056 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| ICNKMEIA_20 | 65321 | 66052 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011227248 |
| ICNKMEIA_5 | 35186 | 36457 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.48 | 99.76 | WP_011226331 |
| ICNKMEIA_20 | 63860 | 65320 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.26 | 100.21 | WP_011226049 |
| ICNKMEIA_20 | 63860 | 65320 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.30 | 100.21 | WP_041827043 |
| ICNKMEIA_20 | 66766 | 67524 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 95.52 | 100.94 | WP_011681174 |
| ICNKMEIA_20 | 66766 | 67524 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 95.39 | 100.94 | WP_011226046 |
| ICNKMEIA_20 | 67581 | 68946 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.24 | 99.85 | WP_011681173 |
| ICNKMEIA_20 | 66766 | 67519 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 94.56 | 99.07 | WP_011227246 |
| ICNKMEIA_20 | 67581 | 68948 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.01 | 100.00 | WP_011226045 |
| ICNKMEIA_20 | 66061 | 66756 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.08 | 100.29 | WP_011681175 |
| ICNKMEIA_41 | 76117 | 77420 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| ICNKMEIA_58 | 12651 | 13661 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.44 | 99.41 | WP_002986042 |
| ICNKMEIA_50 | 63720 | 65003 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| ICNKMEIA_17 | 21403 | 22449 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ICNKMEIA_11 | 27608 | 26625 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| ICNKMEIA_40 | 13785 | 12712 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| ICNKMEIA_41 | 161105 | 160137 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| ICNKMEIA_2 | 15666 | 13363 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| ICNKMEIA_8 | 79614 | 78133 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| ICNKMEIA_39 | 11392 | 9146 | metE | 0.0 | methionine biosynthesis enzymes | 91.72 | 100.00 | AAK74738 |
| ICNKMEIA_54 | 2015 | 1017 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| ICNKMEIA_55 | 27455 | 26484 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| ICNKMEIA_4 | 73229 | 74380 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| ICNKMEIA_5 | 18262 | 20250 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| ICNKMEIA_11 | 43816 | 45546 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| ICNKMEIA_50 | 103923 | 105401 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| ICNKMEIA_41 | 77420 | 76119 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| ICNKMEIA_50 | 119112 | 121004 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| ICNKMEIA_39 | 6221 | 7930 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| ICNKMEIA_34 | 2109 | 1099 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| ICNKMEIA_27 | 68435 | 69796 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| ICNKMEIA_12 | 26127 | 28019 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| ICNKMEIA_41 | 91855 | 90965 | bglB | 1.81e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| ICNKMEIA_31 | 43976 | 44914 | SP_0676 | 6.01e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| ICNKMEIA_39 | 8954 | 8097 | metF | 1.47e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| ICNKMEIA_20 | 27059 | 27763 | covR | 1.73e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| ICNKMEIA_8 | 120806 | 121477 | rr01 | 2.09e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| ICNKMEIA_20 | 27059 | 27763 | walR | 7.30e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| ICNKMEIA_39 | 2267 | 1599 | CiaR | 1.96e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| ICNKMEIA_48 | 8207 | 7521 | CovR | 4.42e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| ICNKMEIA_39 | 2267 | 1596 | ciaR | 1.93e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| ICNKMEIA_54 | 741 | 367 | SpxA2 | 9.50e-72 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| ICNKMEIA_27 | 57075 | 56674 | SpxA1 | 2.53e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| ICNKMEIA_47 | 25528 | 25241 | spr0479 | 3.04e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| ICNKMEIA_41 | 195518 | 195264 | spr1327 | 3.75e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| ICNKMEIA_50 | 174366 | 174103 | spr0175 | 8.80e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| ICNKMEIA_50 | 173539 | 173273 | spr0177 | 1.56e-33 | essential gene | 84.27 | 88.12 | AAK98981 |
| ICNKMEIA_50 | 63562 | 63062 | RopE (M5005_Spy_1611) | 4.89e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| ICNKMEIA_2 | 15666 | 13369 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| ICNKMEIA_12 | 40599 | 41618 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ICNKMEIA_1 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_2 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_3 | 1.0 | 0.0 | 1.0 |
| ICNKMEIA_4 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_5 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_6 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_8 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_9 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_10 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_11 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_12 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_13 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_16 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_17 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_19 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_20 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_21 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_24 | 1.0 | 0.0 | 1.0 |
| ICNKMEIA_26 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_27 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_28 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_31 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_32 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_33 | 1.0 | 0.0 | 1.0 |
| ICNKMEIA_34 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_35 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_37 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_38 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_39 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_40 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_41 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_42 | 1.0 | 0.0 | 1.0 |
| ICNKMEIA_43 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_44 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_45 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_46 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_47 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_48 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_49 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_50 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_52 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_54 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_55 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_56 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_58 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_59 | 0.0 | 1.0 | 0.0 |
| ICNKMEIA_60 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)