safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BJOMJGKA_1	1138358	1139227	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
BJOMJGKA_1	1137765	1138358	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
BJOMJGKA_1	1137765	1138358	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
BJOMJGKA_1	1353317	1354273	STU_RS16250	Capsule	Immune modulation	99.79	100.00	WP_011226328
BJOMJGKA_1	1352524	1353333	STU_RS16245	Capsule	Immune modulation	99.75	100.00	WP_002948020
BJOMJGKA_1	1137765	1138358	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
BJOMJGKA_1	1354270	1355418	STU_RS16255	Capsule	Immune modulation	99.65	100.00	WP_041828290
BJOMJGKA_1	904079	905737	fbp54	FBPs	Adherence	99.58	100.00	WP_011227179
BJOMJGKA_1	1351319	1352524	STU_RS16240	Capsule	Immune modulation	99.58	100.00	WP_011226326
BJOMJGKA_1	1348463	1350247	STU_RS16230	Capsule	Immune modulation	99.55	100.00	WP_011226325
BJOMJGKA_1	1358914	1359765	rfbD	Capsule	Immune modulation	99.53	100.00	WP_014621820
BJOMJGKA_1	43703	44542	cbpD	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
BJOMJGKA_1	1355527	1356537	STU_RS16260	Capsule	Immune modulation	99.50	100.00	WP_011226330
BJOMJGKA_1	1350244	1351299	STU_RS16235	Capsule	Immune modulation	99.43	100.00	WP_002948022
BJOMJGKA_1	1016284	1017015	STER_RS05315	Capsule	Immune modulation	99.04	100.00	WP_011681176
BJOMJGKA_1	1016284	1017015	STU_RS14645	Capsule	Immune modulation	98.77	100.00	WP_011226048
BJOMJGKA_1	1016284	1017015	STR_RS05260	Capsule	Immune modulation	98.63	100.00	WP_011227248
BJOMJGKA_1	1082871	1083659	cshA	Cell surface hydrophobicity proteins	Adherence	98.36	99.75	-
BJOMJGKA_1	1006343	1007758	STER_RS05250	Capsule	Immune modulation	97.81	100.00	WP_011681165
BJOMJGKA_1	1174550	1175311	srtA	Sortase A	Exoenzyme	97.64	100.00	-
BJOMJGKA_1	1356540	1357811	STU_RS16265	Capsule	Immune modulation	97.48	99.76	WP_011226331
BJOMJGKA_1	1017016	1018476	STER_RS05320	Capsule	Immune modulation	97.47	100.00	WP_011681177
BJOMJGKA_1	1017016	1018476	STU_RS14650	Capsule	Immune modulation	96.85	100.21	WP_011226049
BJOMJGKA_1	1017016	1018476	STR_RS05265	Capsule	Immune modulation	95.89	100.21	WP_041827043
BJOMJGKA_1	1015583	1016275	STR_RS05255	Capsule	Immune modulation	95.40	99.86	WP_011227247
BJOMJGKA_1	1015583	1016275	STU_RS14640	Capsule	Immune modulation	95.40	99.86	WP_011226047
BJOMJGKA_1	1013393	1014758	STER_RS05300	Capsule	Immune modulation	95.17	99.85	WP_011681173
BJOMJGKA_1	1014815	1015573	STER_RS05305	Capsule	Immune modulation	93.94	100.94	WP_011681174
BJOMJGKA_1	1013391	1014758	STU_RS14630	Capsule	Immune modulation	93.93	100.00	WP_011226045
BJOMJGKA_1	1014815	1015573	STU_RS14635	Capsule	Immune modulation	93.81	100.94	WP_011226046
BJOMJGKA_1	1014820	1015573	STR_RS05250	Capsule	Immune modulation	93.24	99.07	WP_011227246
BJOMJGKA_1	1015583	1016275	STER_RS05310	Capsule	Immune modulation	89.47	100.00	WP_011681175
BJOMJGKA_1	640615	641918	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
BJOMJGKA_1	1648876	1649886	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	87.45	99.90	WP_002986042
BJOMJGKA_1	160749	162032	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
BJOMJGKA_1	1136290	1137336	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BJOMJGKA_1	1177998	1178981	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
BJOMJGKA_1	744203	745276	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
BJOMJGKA_1	557216	558184	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
BJOMJGKA_1	1525854	1528157	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
BJOMJGKA_1	1253363	1254844	SPD_0310	0.0	UPF0371 protein SPD_0310	92.31	100.00	ABJ54480
BJOMJGKA_1	780647	782893	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
BJOMJGKA_1	65890	66882	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
BJOMJGKA_1	27219	28190	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
BJOMJGKA_1	1412068	1410917	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
BJOMJGKA_1	1375056	1373068	uvrB	0.0	excinuclease ABC, B subunit	88.99	100.00	AE014259_2
BJOMJGKA_1	1161802	1160072	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
BJOMJGKA_1	1856870	1855392	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
BJOMJGKA_1	640615	641916	Eno	0.0	enolase	86.21	99.77	ABP90468
BJOMJGKA_1	1841679	1839787	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
BJOMJGKA_1	785818	784109	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
BJOMJGKA_1	1692546	1693556	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
BJOMJGKA_1	930030	928669	mnmE	0.0	central tRNA-modifying GTPase	81.80	99.56	ABP90420
BJOMJGKA_1	1744241	1742349	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
BJOMJGKA_1	626180	627070	bglB	1.34e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
BJOMJGKA_1	845184	844246	SP_0676	2.59e-167	transcription facot	81.15	97.20	AAK74821
BJOMJGKA_1	783085	783942	metF	8.71e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
BJOMJGKA_1	1055184	1054480	covR	4.41e-131	responder protein	85.53	100.00	AAK71319
BJOMJGKA_1	1234386	1233715	rr01	3.03e-124	response regulator	83.93	99.56	CAB54566
BJOMJGKA_1	1055184	1054480	walR	1.86e-121	DNA-binding response regulator	82.13	100.00	ANC99504
BJOMJGKA_1	789772	790440	CiaR	9.48e-118	two-component response regulator	84.75	99.55	AAK74935
BJOMJGKA_1	315112	315798	CovR	1.01e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
BJOMJGKA_1	789772	790443	ciaR	1.03e-115	two component system response regulator	82.59	99.12	AIK75708
BJOMJGKA_1	67194	67589	SpxA2	3.21e-73	transcriptional regulator	90.15	100.00	ERL21461
BJOMJGKA_1	941389	941790	SpxA1	3.79e-66	transcriptional regulator	80.60	100.00	ERL19273
BJOMJGKA_1	162190	162690	RopE (M5005_Spy_1611)	4.96e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
BJOMJGKA_1	1525854	1528151	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
BJOMJGKA_1	1729832	1728813	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	95.88	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BJOMJGKA_1	0.0	1.0	0.0
BJOMJGKA_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found