Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KEAJKEGP_1 | 3987522 | 3988436 | mphK | ARO:3004541 | mphK | 96.08 | 99.35 | CP010314.1 |
| KEAJKEGP_1 | 3977015 | 3978448 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| KEAJKEGP_1 | 3900254 | 3900847 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| KEAJKEGP_1 | 3637619 | 3639262 | vmlR | ARO:3004476 | vmlR | 97.99 | 100.00 | NC_000964.3 |
| KEAJKEGP_1 | 3311549 | 3314119 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| KEAJKEGP_1 | 2876690 | 2877028 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| KEAJKEGP_1 | 2876373 | 2876690 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| KEAJKEGP_1 | 1868867 | 1870036 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KEAJKEGP_2 | 8114 | 8958 | blaZ | Amoxicillin, Ampicillin, Penicillin, Piperacillin | 99.88 | 99.88 | AJ302698 |
| KEAJKEGP_1 | 3987522 | 3988436 | mph(K) | Spiramycin, Telithromycin | 96.85 | 99.35 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KEAJKEGP_2 | 9065 | 10819 | blaR1 | beta-lactam sensor/signal transducer BlaR1 | EXACTX | 100.00 | 100.00 | WP_001096386.1 |
| KEAJKEGP_2 | 8117 | 8866 | blaZ | penicillin-hydrolyzing class A beta-lactamase BlaZ | PARTIALX | 100.00 | 88.97 | WP_000733274.1 |
| KEAJKEGP_2 | 10812 | 11189 | blaI | penicillinase repressor BlaI | BLASTX | 99.21 | 100.00 | WP_001284656.1 |
| KEAJKEGP_1 | 3637619 | 3639259 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.99 | 100.00 | WP_003234144.1 |
| KEAJKEGP_1 | 3987525 | 3988436 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.08 | 100.00 | WP_003246254.1 |
| KEAJKEGP_1 | 187125 | 187643 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KEAJKEGP_1 | 668373 | 669515 | capA | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | NP_391469 |
| KEAJKEGP_1 | 1137180 | 1138118 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| KEAJKEGP_1 | 669998 | 671179 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| KEAJKEGP_1 | 669534 | 669983 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| KEAJKEGP_1 | 2071573 | 2072214 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| KEAJKEGP_1 | 1133508 | 1134293 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| KEAJKEGP_1 | 2335106 | 2336869 | dep/capD | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | NP_389723 |
| KEAJKEGP_1 | 1138138 | 1145274 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391076 |
| KEAJKEGP_1 | 1134319 | 1135515 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| KEAJKEGP_1 | 1135533 | 1137152 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.84 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KEAJKEGP_1 | 27990 | 26854 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| KEAJKEGP_1 | 142090 | 141221 | pdxS | 1.02e-148 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| KEAJKEGP_1 | 2538666 | 2537890 | CodY | 3.33e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KEAJKEGP_1 | 836318 | 835749 | ClpP | 2.47e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KEAJKEGP_2 | 1 | 944 | rep19b | 944 / 969 | 92.69 | GQ900452 |
| KEAJKEGP_2 | 21631 | 22769 | repUS70 | 1139 / 1452 | 92.27 | GQ900405 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KEAJKEGP_1 | 0.0 | 1.0 | 0.0 |
| KEAJKEGP_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KEAJKEGP_1 | 846738 | 873147 | False | Siphoviridae | VOG1183, VOG5495, VOG0275, VOG5023, VOG3699, VOG2613, VOG0692, VOG9317, VOG4319, VOG11074, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG0753, VOG4666, VOG0602, VOG4539, VOG5216, VOG11003, VOG7531 |
| KEAJKEGP_1 | 2114029 | 2130694 | False | Unknown | VOG0054, VOG8537, VOG10230, VOG4358, VOG9712, VOG5042, VOG5042, VOG3389, VOG4910, VOG9312 |
| KEAJKEGP_1 | 2133995 | 2148337 | True | Unknown | VOG0275, VOG7930, VOG8610, VOG7895, VOG6243, VOG7718, VOG5534, VOG6940, VOG9713, VOG9219, VOG0944, VOG5532, VOG6036, VOG9867, VOG7134, VOG10940 |
| KEAJKEGP_1 | 2906284 | 2932142 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| KEAJKEGP_1 | 3552438 | 3570946 | False | Siphoviridae | VOG5535, VOG4599, VOG4605, VOG9745, VOG0701, VOG10044, VOG0698, VOG0697, VOG0696, VOG0695, VOG2621, VOG4572, VOG9317, VOG0692, VOG0691, VOG10227, VOG0796 |
| KEAJKEGP_1 | 3576299 | 3588991 | False | Siphoviridae | VOG0586, VOG0189, VOG4566, VOG1309, VOG4600, VOG0125, VOG1878, VOG4552, VOG0650, VOG9667, VOG9667, VOG4662, VOG4602, VOG0275, VOG6513, VOG6585 |
Phigaro v2.4.0 (Database: Jan. 2024 release)