Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KAALHNMO_66 | 141070 | 141990 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| KAALHNMO_66 | 127736 | 129175 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| KAALHNMO_66 | 78079 | 78672 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| KAALHNMO_92 | 59648 | 60817 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| KAALHNMO_7 | 53780 | 54118 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KAALHNMO_7 | 53463 | 53780 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| KAALHNMO_111 | 27275 | 29845 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| KAALHNMO_67 | 9326 | 10528 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| KAALHNMO_109 | 8345 | 9988 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| KAALHNMO_114 | 141 | 995 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KAALHNMO_114 | 141 | 995 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| KAALHNMO_66 | 141070 | 141990 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| KAALHNMO_120 | 36280 | 37656 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KAALHNMO_114 | 141 | 992 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| KAALHNMO_66 | 141073 | 141990 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| KAALHNMO_69 | 1575 | 2093 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| KAALHNMO_120 | 36280 | 37653 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| KAALHNMO_109 | 8348 | 9988 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| KAALHNMO_86 | 1424 | 4018 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KAALHNMO_68 | 14917 | 16059 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| KAALHNMO_68 | 16542 | 17723 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| KAALHNMO_68 | 16078 | 16527 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| KAALHNMO_86 | 48913 | 50676 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| KAALHNMO_119 | 170850 | 171635 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| KAALHNMO_119 | 174533 | 175471 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| KAALHNMO_119 | 171661 | 172857 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| KAALHNMO_119 | 172886 | 174505 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| KAALHNMO_119 | 175491 | 182627 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391076 |
| KAALHNMO_51 | 30844 | 31485 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KAALHNMO_20 | 31692 | 32828 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KAALHNMO_18 | 11202 | 10333 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KAALHNMO_16 | 161371 | 160595 | CodY | 2.57e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KAALHNMO_126 | 79754 | 80323 | ClpP | 6.57e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| KAALHNMO_6 | 144987 | 144595 | spxA1 | 3.87e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| KAALHNMO_2 | 34595 | 34353 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| KAALHNMO_73 | 533 | 336 | CspA | 1.08e-35 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| KAALHNMO_131 | 124331 | 124137 | CspD | 7.71e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| KAALHNMO_106 | 33223 | 33029 | CspB | 6.22e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KAALHNMO_1 | 1.0 | 0.0 | 1.0 |
| KAALHNMO_2 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_3 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_5 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_6 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_7 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_8 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_9 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_10 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_11 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_13 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_14 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_16 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_17 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_18 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_19 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_20 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_23 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_24 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_25 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_26 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_27 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_28 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_30 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_31 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_32 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_34 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_36 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_37 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_38 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_39 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_40 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_42 | 1.0 | 0.0 | 1.0 |
| KAALHNMO_43 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_44 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_46 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_47 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_48 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_50 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_51 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_52 | 1.0 | 0.0 | 1.0 |
| KAALHNMO_55 | 1.0 | 0.0 | 1.0 |
| KAALHNMO_56 | 1.0 | 0.0 | 1.0 |
| KAALHNMO_57 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_58 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_59 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_61 | 1.0 | 0.0 | 1.0 |
| KAALHNMO_62 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_63 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_64 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_65 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_66 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_67 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_68 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_69 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_70 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_71 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_72 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_74 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_75 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_76 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_77 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_78 | 1.0 | 0.0 | 1.0 |
| KAALHNMO_79 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_80 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_81 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_82 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_83 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_84 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_85 | 1.0 | 0.0 | 1.0 |
| KAALHNMO_86 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_87 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_89 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_90 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_92 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_93 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_94 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_95 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_97 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_98 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_99 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_100 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_101 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_102 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_103 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_104 | 1.0 | 0.0 | 1.0 |
| KAALHNMO_105 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_106 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_107 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_108 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_109 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_110 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_111 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_112 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_113 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_114 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_115 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_116 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_117 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_118 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_119 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_120 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_123 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_124 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_125 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_126 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_127 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_128 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_129 | 1.0 | 0.0 | 1.0 |
| KAALHNMO_130 | 0.0 | 1.0 | 0.0 |
| KAALHNMO_131 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KAALHNMO_6 | 26015 | 52123 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| KAALHNMO_8 | 1887 | 24187 | False | Siphoviridae | VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641, VOG4918 |
| KAALHNMO_128 | 6838 | 25083 | False | Myoviridae | VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
Phigaro v2.4.0 (Database: Jan. 2024 release)