Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MMHBEFPG_2 | 239460 | 242030 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| MMHBEFPG_98 | 109379 | 109972 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| MMHBEFPG_98 | 58855 | 60294 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| MMHBEFPG_98 | 46041 | 46961 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
| MMHBEFPG_6 | 28996 | 30642 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| MMHBEFPG_99 | 24156 | 24473 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MMHBEFPG_99 | 23818 | 24156 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MMHBEFPG_72 | 12493 | 13347 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| MMHBEFPG_90 | 5256 | 6458 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| MMHBEFPG_18 | 1918 | 3087 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MMHBEFPG_85 | 36390 | 37766 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.93 | 100.00 | X08034 |
| MMHBEFPG_98 | 46041 | 46961 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| MMHBEFPG_72 | 12495 | 13347 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MMHBEFPG_85 | 36390 | 37763 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| MMHBEFPG_98 | 46041 | 46958 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| MMHBEFPG_6 | 28996 | 30639 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| MMHBEFPG_72 | 12496 | 13347 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| MMHBEFPG_85 | 39719 | 40237 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| MMHBEFPG_61 | 8345 | 10942 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MMHBEFPG_91 | 23398 | 24579 | capB | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | NP_391471 |
| MMHBEFPG_5 | 21142 | 28278 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.55 | 100.00 | NP_391076 |
| MMHBEFPG_7 | 42713 | 43354 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| MMHBEFPG_5 | 16501 | 17286 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| MMHBEFPG_5 | 20184 | 21122 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| MMHBEFPG_91 | 21773 | 22915 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| MMHBEFPG_91 | 22934 | 23383 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| MMHBEFPG_11 | 36743 | 38506 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| MMHBEFPG_5 | 17312 | 18508 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.66 | 100.00 | NP_391079 |
| MMHBEFPG_5 | 18537 | 20156 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MMHBEFPG_40 | 31847 | 32983 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MMHBEFPG_74 | 264 | 1133 | pdxS | 8.11e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MMHBEFPG_66 | 93808 | 94584 | CodY | 1.45e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MMHBEFPG_94 | 36012 | 35443 | ClpP | 2.26e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MMHBEFPG_70 | 8415 | 8807 | spxA1 | 2.80e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MMHBEFPG_70 | 8415 | 8807 | spx | 1.67e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MMHBEFPG_86 | 20589 | 20831 | SpoVG | 4.75e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MMHBEFPG_6 | 77464 | 77267 | CspA | 3.47e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MMHBEFPG_51 | 1698 | 1892 | CspD | 5.90e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| MMHBEFPG_59 | 6 | 185 | CspB | 3.64e-27 | cold shock protein | 86.67 | 90.91 | CAD00094 |
| MMHBEFPG_59 | 6 | 185 | msaB | 4.80e-26 | cold shock protein | 80.00 | 90.91 | ABD22065 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MMHBEFPG_1 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_2 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_3 | 1.0 | 0.0 | 1.0 |
| MMHBEFPG_5 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_6 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_7 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_10 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_11 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_13 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_14 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_15 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_17 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_18 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_20 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_21 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_22 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_23 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_24 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_25 | 1.0 | 0.0 | 1.0 |
| MMHBEFPG_26 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_28 | 1.0 | 0.0 | 1.0 |
| MMHBEFPG_30 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_31 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_32 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_33 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_34 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_35 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_38 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_39 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_40 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_41 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_42 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_45 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_46 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_47 | 1.0 | 0.0 | 1.0 |
| MMHBEFPG_48 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_49 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_50 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_51 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_52 | 1.0 | 0.0 | 1.0 |
| MMHBEFPG_53 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_54 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_55 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_56 | 1.0 | 0.0 | 1.0 |
| MMHBEFPG_57 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_58 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_59 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_60 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_61 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_62 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_63 | 1.0 | 0.0 | 1.0 |
| MMHBEFPG_64 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_65 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_66 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_67 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_68 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_69 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_70 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_71 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_72 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_73 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_74 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_75 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_76 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_78 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_79 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_80 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_81 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_83 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_84 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_85 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_86 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_87 | 1.0 | 0.0 | 1.0 |
| MMHBEFPG_88 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_90 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_91 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_92 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_93 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_94 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_95 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_96 | 1.0 | 0.0 | 1.0 |
| MMHBEFPG_97 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_98 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_99 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_100 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_101 | 0.0 | 1.0 | 0.0 |
| MMHBEFPG_102 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MMHBEFPG_13 | 14248 | 36504 | False | Myoviridae | VOG0796, VOG0720, VOG4555, VOG4553, VOG0815, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0641 |
| MMHBEFPG_21 | 98813 | 104434 | False | Siphoviridae | VOG5353, VOG0181, VOG4552, VOG11060, VOG9600, VOG0025 |
| MMHBEFPG_45 | 2848 | 17972 | False | Myoviridae | VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| MMHBEFPG_84 | 2016 | 24428 | False | Siphoviridae | VOG4574, VOG0136, VOG5221, VOG4705, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581 |
| MMHBEFPG_88 | 2128 | 25116 | False | Myoviridae | VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)