Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MLEOMEED_58 | 143349 | 144203 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| MLEOMEED_90 | 68174 | 69613 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| MLEOMEED_102 | 38271 | 39914 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| MLEOMEED_90 | 18517 | 19110 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| MLEOMEED_91 | 7012 | 9582 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| MLEOMEED_27 | 4306 | 5475 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MLEOMEED_19 | 4105 | 4422 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| MLEOMEED_19 | 3767 | 4105 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MLEOMEED_10 | 2388 | 3590 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MLEOMEED_58 | 143349 | 144203 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| MLEOMEED_38 | 248 | 1624 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | X08034 |
| MLEOMEED_90 | 81508 | 82429 | mph(K) | Spiramycin, Telithromycin | 99.89 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MLEOMEED_58 | 143349 | 144200 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| MLEOMEED_87 | 8336 | 8854 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| MLEOMEED_38 | 251 | 1624 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| MLEOMEED_102 | 38271 | 39911 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| MLEOMEED_14 | 5566 | 8160 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
| MLEOMEED_90 | 81809 | 82429 | mphK | macrolide 2'-phosphotransferase MphK | PARTIALX | 98.55 | 67.65 | WP_003246254.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MLEOMEED_96 | 18505 | 19647 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| MLEOMEED_96 | 16841 | 18022 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| MLEOMEED_96 | 18037 | 18486 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| MLEOMEED_101 | 30210 | 31973 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| MLEOMEED_84 | 106 | 891 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| MLEOMEED_40 | 194618 | 195259 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| MLEOMEED_84 | 4747 | 11883 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.99 | 100.00 | NP_391076 |
| MLEOMEED_84 | 3789 | 4727 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| MLEOMEED_84 | 917 | 2113 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.67 | 100.00 | NP_391079 |
| MLEOMEED_84 | 2142 | 3761 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MLEOMEED_7 | 31948 | 33084 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MLEOMEED_64 | 11246 | 10377 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MLEOMEED_82 | 91193 | 91969 | CodY | 7.42e-126 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MLEOMEED_99 | 29914 | 29345 | ClpP | 6.31e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MLEOMEED_21 | 16307 | 16699 | spxA1 | 3.40e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| MLEOMEED_15 | 34595 | 34353 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MLEOMEED_102 | 85386 | 85189 | CspA | 3.75e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MLEOMEED_40 | 182656 | 182462 | CspD | 1.60e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| MLEOMEED_93 | 8276 | 8082 | CspB | 2.01e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MLEOMEED_1 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_2 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_3 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_4 | 1.0 | 0.0 | 1.0 |
| MLEOMEED_6 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_7 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_8 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_9 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_10 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_11 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_12 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_13 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_14 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_15 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_16 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_17 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_19 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_20 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_21 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_22 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_23 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_24 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_25 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_26 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_27 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_29 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_31 | 1.0 | 0.0 | 1.0 |
| MLEOMEED_33 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_34 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_35 | 1.0 | 0.0 | 1.0 |
| MLEOMEED_36 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_37 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_38 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_40 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_41 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_42 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_43 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_44 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_46 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_47 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_48 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_49 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_51 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_52 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_54 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_55 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_56 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_57 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_58 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_60 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_61 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_62 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_63 | 1.0 | 0.0 | 1.0 |
| MLEOMEED_64 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_65 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_66 | 1.0 | 0.0 | 1.0 |
| MLEOMEED_67 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_68 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_69 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_70 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_71 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_72 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_74 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_75 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_77 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_78 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_79 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_80 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_81 | 1.0 | 0.0 | 1.0 |
| MLEOMEED_82 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_83 | 1.0 | 0.0 | 1.0 |
| MLEOMEED_84 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_85 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_86 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_87 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_88 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_89 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_90 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_91 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_93 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_94 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_95 | 1.0 | 0.0 | 1.0 |
| MLEOMEED_96 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_97 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_98 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_99 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_100 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_101 | 0.0 | 1.0 | 0.0 |
| MLEOMEED_102 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MLEOMEED_21 | 109183 | 135291 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| MLEOMEED_27 | 176535 | 193793 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589 |
| MLEOMEED_44 | 7314 | 11291 | False | Siphoviridae | VOG4552, VOG0181, VOG5353, VOG9667 |
| MLEOMEED_47 | 10760 | 33060 | False | Siphoviridae | VOG1659, VOG4918, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886 |
Phigaro v2.4.0 (Database: Jan. 2024 release)