Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 1 | 4 | 6.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BDNNAKAI_8 | 270433 | 271485 | clbC | ARO:3002816 | clbC | 100.00 | 100.00 | AP006627.1 |
| BDNNAKAI_6 | 222738 | 223662 | BCL-1 | ARO:3004749 | BCL-1 | 100.00 | 98.05 | EF540343.1 |
| BDNNAKAI_6 | 219572 | 220342 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 96.88 | 100.00 | EF540343.1 |
| BDNNAKAI_1 | 196272 | 197117 | Erm(34) | ARO:3000600 | Erm(34) | 96.44 | 100.00 | AY234334.1 |
| BDNNAKAI_10 | 94099 | 94746 | Alkalihalobacillus clausii chloramphenicol acetyltransferase | ARO:3004452 | Acla_ACT_CHL | 88.68 | 94.30 | AY238971.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BDNNAKAI_10 | 94068 | 94744 | cat | Chloramphenicol | 97.52 | 98.40 | AY238971 |
| BDNNAKAI_6 | 219572 | 220342 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 97.28 | 100.00 | AJ506108 |
| BDNNAKAI_1 | 196272 | 197110 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 95.35 | 99.17 | AY234334 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BDNNAKAI_6 | 222756 | 223658 | blaBCL-1 | class A beta-lactamase BCL-1 | ALLELEX | 100.00 | 100.00 | WP_065825670.1 |
| BDNNAKAI_10 | 94102 | 94746 | catA | type A-10 chloramphenicol O-acetyltransferase | EXACTX | 100.00 | 100.00 | WP_035203840.1 |
| BDNNAKAI_8 | 270436 | 271485 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | EXACTX | 100.00 | 100.00 | WP_011245929.1 |
| BDNNAKAI_6 | 219572 | 220339 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 96.88 | 100.00 | WP_035202393.1 |
| BDNNAKAI_1 | 196272 | 197111 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 96.79 | 99.64 | WP_063844504.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BDNNAKAI_2 | 39328 | 39978 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.85 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BDNNAKAI_14 | 32623 | 33759 | tufA | 0.0 | elongation factor Tu | 87.33 | 95.69 | AHM69299 |
| BDNNAKAI_13 | 42514 | 42272 | SpoVG | 2.56e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BDNNAKAI_7 | 6341 | 6147 | CspA | 1.48e-29 | cold shock protein | 86.15 | 98.48 | CAA62903 |
| BDNNAKAI_2 | 535005 | 534814 | msaB | 1.61e-25 | cold shock protein | 81.25 | 96.97 | ABD22065 |
| BDNNAKAI_2 | 535002 | 534814 | CspB | 1.39e-24 | cold shock protein | 80.95 | 95.45 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BDNNAKAI_1 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_2 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_3 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_4 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_5 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_6 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_7 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_8 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_9 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_10 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_11 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_12 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_13 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_14 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_15 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_16 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_17 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_19 | 1.0 | 0.0 | 1.0 |
| BDNNAKAI_20 | 0.0 | 1.0 | 0.0 |
| BDNNAKAI_21 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BDNNAKAI_6 | 306822 | 331650 | False | Siphoviridae | VOG4972, VOG8236, VOG0641, VOG7454, VOG4619, VOG4669, VOG0801, VOG0648, VOG4662, VOG0701, VOG0700, VOG0698, VOG0697, VOG0696, VOG0695, VOG1322, VOG1329, VOG4555, VOG0692, VOG0691, VOG4544, VOG0796 |
| BDNNAKAI_6 | 336866 | 345037 | False | Siphoviridae | VOG0052, VOG6587, VOG0189, VOG4799, VOG10059, VOG11077, VOG1563, VOG4561, VOG7719, VOG2913, VOG10847, VOG5407 |
Phigaro v2.4.0 (Database: Jan. 2024 release)