Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 1 | 4 | 6.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LOFBCHLA_1 | 192247 | 193092 | Erm(34) | ARO:3000600 | Erm(34) | 96.80 | 100.00 | AY234334.1 |
| LOFBCHLA_4 | 113265 | 114317 | clbC | ARO:3002816 | clbC | 100.00 | 100.00 | AP006627.1 |
| LOFBCHLA_8 | 29102 | 29749 | Alkalihalobacillus clausii chloramphenicol acetyltransferase | ARO:3004452 | Acla_ACT_CHL | 88.21 | 94.30 | AY238971.1 |
| LOFBCHLA_19 | 28895 | 29800 | BCL-1 | ARO:3004749 | BCL-1 | 99.67 | 98.05 | EF540343.1 |
| LOFBCHLA_19 | 25711 | 26481 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 98.05 | 100.00 | EF540343.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| LOFBCHLA_19 | 25711 | 26481 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 98.96 | 100.00 | AJ506108 |
| LOFBCHLA_8 | 29104 | 29780 | cat | Chloramphenicol | 97.23 | 98.40 | AY238971 |
| LOFBCHLA_1 | 192247 | 193079 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 95.92 | 98.46 | AY234334 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LOFBCHLA_4 | 113268 | 114317 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | EXACTX | 100.00 | 100.00 | WP_011245929.1 |
| LOFBCHLA_19 | 28895 | 29797 | blaBCL | BCL family class A beta-lactamase | BLASTX | 99.67 | 100.00 | WP_065825670.1 |
| LOFBCHLA_8 | 29102 | 29746 | catA | type A-10 chloramphenicol O-acetyltransferase | BLASTX | 99.53 | 100.00 | WP_035203840.1 |
| LOFBCHLA_19 | 25711 | 26478 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 98.05 | 100.00 | WP_035202393.1 |
| LOFBCHLA_1 | 192247 | 193086 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 97.14 | 99.64 | WP_063844504.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LOFBCHLA_14 | 46794 | 47444 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LOFBCHLA_20 | 41560 | 40424 | tufA | 0.0 | elongation factor Tu | 87.33 | 95.69 | AHM69299 |
| LOFBCHLA_15 | 43049 | 42807 | SpoVG | 2.70e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| LOFBCHLA_27 | 6178 | 6372 | CspA | 1.64e-30 | cold shock protein | 86.15 | 98.48 | CAA62903 |
| LOFBCHLA_1 | 651736 | 651927 | msaB | 1.98e-25 | cold shock protein | 81.25 | 96.97 | ABD22065 |
| LOFBCHLA_1 | 651739 | 651927 | CspB | 1.70e-24 | cold shock protein | 80.95 | 95.45 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LOFBCHLA_1 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_2 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_3 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_4 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_5 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_6 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_7 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_8 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_9 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_10 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_11 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_12 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_13 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_14 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_15 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_16 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_17 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_18 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_19 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_20 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_21 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_22 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_23 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_24 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_25 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_26 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_27 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_28 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_29 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_30 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_31 | 1.0 | 0.0 | 1.0 |
| LOFBCHLA_33 | 1.0 | 0.0 | 1.0 |
| LOFBCHLA_34 | 1.0 | 0.0 | 1.0 |
| LOFBCHLA_35 | 1.0 | 0.0 | 1.0 |
| LOFBCHLA_36 | 0.0 | 1.0 | 0.0 |
| LOFBCHLA_38 | 1.0 | 0.0 | 1.0 |
| LOFBCHLA_39 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| LOFBCHLA_11 | 16272 | 53444 | False | Siphoviridae | VOG10137, VOG4841, VOG4581, VOG4544, VOG3607, VOG4556, VOG4568, VOG4553, VOG7168, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG4910, VOG0801, VOG4660, VOG5384, VOG3390, VOG4705, VOG4619, VOG0641, VOG8236, VOG0018, VOG6116, VOG0866, VOG4662, VOG5532, VOG4552, VOG0468, VOG6414, VOG6931, VOG0535, VOG1936, VOG4606, VOG2752, VOG4559 |
Phigaro v2.4.0 (Database: Jan. 2024 release)