Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 21 | 35 | 40.87 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DCHGNEAJ_10 | 302429 | 303646 | mef(A) | Erythromycin, Azithromycin | 100.00 | 100.00 | U83667 |
| DCHGNEAJ_10 | 303766 | 305229 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | AF274302 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DCHGNEAJ_10 | 302429 | 303643 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| DCHGNEAJ_10 | 303766 | 305226 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | EXACTX | 100.00 | 100.00 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DCHGNEAJ_10 | 611285 | 612025 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.87 | 100.00 | WP_011681174 |
| DCHGNEAJ_21 | 98151 | 99002 | rfbD | 0.0 | Capsule | Immune modulation | 94.60 | 100.00 | WP_014621820 |
| DCHGNEAJ_10 | 608382 | 609842 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 94.59 | 100.00 | WP_011681177 |
| DCHGNEAJ_10 | 612082 | 613447 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.44 | 99.85 | WP_011681173 |
| DCHGNEAJ_10 | 608382 | 609842 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.39 | 100.21 | WP_011226049 |
| DCHGNEAJ_10 | 611285 | 612025 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 94.20 | 100.00 | WP_011226046 |
| DCHGNEAJ_10 | 612082 | 613449 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.08 | 100.00 | WP_011226045 |
| DCHGNEAJ_10 | 608382 | 609842 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.77 | 100.21 | WP_041827043 |
| DCHGNEAJ_10 | 611285 | 612020 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.61 | 98.13 | WP_011227246 |
| DCHGNEAJ_21 | 95154 | 95963 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 92.72 | 100.00 | WP_002948020 |
| DCHGNEAJ_21 | 93949 | 95154 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.54 | 100.00 | WP_011226326 |
| DCHGNEAJ_21 | 91130 | 92886 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.43 | 98.43 | WP_011226325 |
| DCHGNEAJ_21 | 95947 | 96903 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.27 | 100.00 | WP_011226328 |
| DCHGNEAJ_10 | 609843 | 610574 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 92.21 | 100.00 | WP_011226048 |
| DCHGNEAJ_10 | 441916 | 442509 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.75 | 100.00 | WP_011226149 |
| DCHGNEAJ_21 | 96900 | 98048 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 91.74 | 99.91 | WP_041828290 |
| DCHGNEAJ_10 | 441916 | 442509 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.58 | 100.00 | WP_011226149 |
| DCHGNEAJ_10 | 441916 | 442509 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.58 | 100.00 | WP_011226149 |
| DCHGNEAJ_10 | 609843 | 610574 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011681176 |
| DCHGNEAJ_10 | 441047 | 441916 | rfbA | 0.0 | Capsule | Immune modulation | 91.49 | 100.00 | WP_011681286 |
| DCHGNEAJ_21 | 92883 | 93929 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.38 | 99.53 | WP_002948022 |
| DCHGNEAJ_10 | 609843 | 610574 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 91.26 | 100.00 | WP_011227248 |
| DCHGNEAJ_10 | 730366 | 732024 | fbp54 | 0.0 | FBPs | Adherence | 90.24 | 99.94 | WP_011227179 |
| DCHGNEAJ_10 | 85597 | 86502 | cbpD | 0.0 | Autolysin | Exoenzyme | 88.52 | 93.50 | WP_082302295 |
| DCHGNEAJ_21 | 393052 | 394062 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.35 | 99.80 | WP_002986042 |
| DCHGNEAJ_10 | 265194 | 266497 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| DCHGNEAJ_10 | 610583 | 611275 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.18 | 99.86 | WP_011227247 |
| DCHGNEAJ_10 | 610583 | 611275 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.18 | 99.86 | WP_011226047 |
| DCHGNEAJ_10 | 610583 | 611275 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.18 | 99.86 | WP_011681175 |
| DCHGNEAJ_10 | 882509 | 883270 | srtA | 0.0 | Sortase A | Exoenzyme | 85.32 | 99.87 | - |
| DCHGNEAJ_10 | 442712 | 443758 | rfbB | 0.0 | Capsule | Immune modulation | 84.35 | 99.90 | WP_002263087 |
| DCHGNEAJ_32 | 203426 | 204709 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.31 | 99.61 | WP_011054989 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DCHGNEAJ_10 | 885960 | 886943 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| DCHGNEAJ_10 | 874375 | 873302 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| DCHGNEAJ_21 | 272786 | 275092 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| DCHGNEAJ_10 | 1005482 | 1006963 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| DCHGNEAJ_10 | 855316 | 853070 | metE | 0.0 | methionine biosynthesis enzymes | 93.06 | 100.00 | AAK74738 |
| DCHGNEAJ_10 | 174968 | 175936 | spr0084 | 0.0 | pathogenitcity related | 92.26 | 98.48 | AAK98888 |
| DCHGNEAJ_2 | 38921 | 37950 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| DCHGNEAJ_10 | 415657 | 417387 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| DCHGNEAJ_21 | 111396 | 109408 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| DCHGNEAJ_21 | 139874 | 138723 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| DCHGNEAJ_33 | 117749 | 116271 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| DCHGNEAJ_10 | 265194 | 266495 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| DCHGNEAJ_33 | 102396 | 100504 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| DCHGNEAJ_10 | 850149 | 851858 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| DCHGNEAJ_2 | 2037 | 946 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| DCHGNEAJ_34 | 28500 | 29510 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| DCHGNEAJ_10 | 704364 | 705725 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.89 | 99.56 | ABP90420 |
| DCHGNEAJ_10 | 250784 | 251674 | bglB | 5.89e-177 | putative phospho--glucosidase | 87.54 | 99.66 | AAK33487 |
| DCHGNEAJ_10 | 810863 | 811813 | SP_0676 | 1.16e-169 | transcription facot | 81.39 | 98.45 | AAK74821 |
| DCHGNEAJ_10 | 852880 | 852023 | metF | 3.40e-169 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| DCHGNEAJ_35 | 28668 | 29348 | NisR | 2.05e-132 | two-component signal-transduction systems | 94.27 | 100.00 | ABP89873 |
| DCHGNEAJ_35 | 29336 | 30040 | NisK | 7.00e-131 | two-component signal-transduction systems | 90.64 | 100.00 | ABP89872 |
| DCHGNEAJ_10 | 563579 | 564283 | covR | 5.10e-127 | responder protein | 85.96 | 100.00 | AAK71319 |
| DCHGNEAJ_10 | 957115 | 956444 | rr01 | 8.30e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| DCHGNEAJ_10 | 846182 | 845511 | ciaR | 1.18e-124 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| DCHGNEAJ_10 | 846182 | 845514 | CiaR | 8.90e-124 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| DCHGNEAJ_32 | 55403 | 54717 | CovR | 4.51e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| DCHGNEAJ_10 | 563579 | 564283 | walR | 9.27e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| DCHGNEAJ_2 | 773 | 378 | SpxA2 | 4.24e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| DCHGNEAJ_10 | 692896 | 692495 | SpxA1 | 2.11e-69 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| DCHGNEAJ_38 | 6220 | 5807 | GrpE | 4.22e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| DCHGNEAJ_32 | 32192 | 31905 | spr0479 | 5.47e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| DCHGNEAJ_10 | 136764 | 137018 | spr1327 | 4.67e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| DCHGNEAJ_33 | 58905 | 59168 | spr0175 | 7.42e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| DCHGNEAJ_33 | 59729 | 59995 | spr0177 | 1.65e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| DCHGNEAJ_32 | 203259 | 202768 | RopE (M5005_Spy_1611) | 9.99e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| DCHGNEAJ_21 | 272786 | 275086 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| DCHGNEAJ_34 | 64929 | 63910 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DCHGNEAJ_1 | 1.0 | 0.0 | 1.0 |
| DCHGNEAJ_2 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_3 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_4 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_5 | 1.0 | 0.0 | 1.0 |
| DCHGNEAJ_7 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_8 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_9 | 1.0 | 0.0 | 1.0 |
| DCHGNEAJ_10 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_11 | 1.0 | 0.0 | 1.0 |
| DCHGNEAJ_12 | 1.0 | 0.0 | 1.0 |
| DCHGNEAJ_13 | 1.0 | 0.0 | 1.0 |
| DCHGNEAJ_14 | 1.0 | 0.0 | 1.0 |
| DCHGNEAJ_15 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_21 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_32 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_33 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_34 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_35 | 1.0 | 0.0 | 1.0 |
| DCHGNEAJ_36 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_37 | 0.0 | 1.0 | 0.0 |
| DCHGNEAJ_38 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)