Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DMACJMHG_65 | 107823 | 108743 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| DMACJMHG_65 | 94489 | 95928 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| DMACJMHG_65 | 44832 | 45425 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| DMACJMHG_60 | 29094 | 30737 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| DMACJMHG_98 | 28910 | 31480 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| DMACJMHG_56 | 21337 | 21675 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DMACJMHG_56 | 21020 | 21337 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| DMACJMHG_89 | 8998 | 9852 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| DMACJMHG_104 | 2194 | 3363 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DMACJMHG_92 | 886 | 2088 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DMACJMHG_89 | 8998 | 9852 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| DMACJMHG_65 | 107823 | 108743 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| DMACJMHG_18 | 435 | 1811 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DMACJMHG_89 | 8998 | 9849 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| DMACJMHG_65 | 107826 | 108743 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| DMACJMHG_116 | 1575 | 2093 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| DMACJMHG_18 | 438 | 1811 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| DMACJMHG_60 | 29094 | 30734 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| DMACJMHG_91 | 14465 | 17059 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DMACJMHG_109 | 88620 | 89762 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| DMACJMHG_109 | 86956 | 88137 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| DMACJMHG_109 | 88152 | 88601 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| DMACJMHG_75 | 6619 | 8382 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| DMACJMHG_63 | 26691 | 27476 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| DMACJMHG_63 | 22855 | 23793 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| DMACJMHG_63 | 25469 | 26665 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| DMACJMHG_63 | 23821 | 25440 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| DMACJMHG_63 | 15699 | 22835 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391076 |
| DMACJMHG_97 | 7214 | 7855 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DMACJMHG_71 | 15589 | 14453 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DMACJMHG_105 | 4286 | 5155 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DMACJMHG_76 | 25776 | 25000 | CodY | 1.12e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DMACJMHG_131 | 68633 | 69202 | ClpP | 5.91e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DMACJMHG_20 | 94043 | 93651 | spxA1 | 2.26e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| DMACJMHG_113 | 1821 | 1579 | SpoVG | 1.64e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DMACJMHG_118 | 8879 | 8682 | CspA | 4.62e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DMACJMHG_87 | 103003 | 102809 | CspD | 6.42e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| DMACJMHG_69 | 17381 | 17187 | CspB | 4.74e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DMACJMHG_1 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_2 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_3 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_4 | 1.0 | 0.0 | 1.0 |
| DMACJMHG_5 | 1.0 | 0.0 | 1.0 |
| DMACJMHG_6 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_7 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_8 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_9 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_10 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_11 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_12 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_13 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_14 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_15 | 1.0 | 0.0 | 1.0 |
| DMACJMHG_16 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_17 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_18 | 1.0 | 0.0 | 1.0 |
| DMACJMHG_19 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_20 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_21 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_22 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_23 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_24 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_25 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_27 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_32 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_33 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_35 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_36 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_38 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_46 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_47 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_55 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_56 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_57 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_58 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_59 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_60 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_61 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_62 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_63 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_64 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_65 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_66 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_67 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_68 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_69 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_70 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_71 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_72 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_73 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_74 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_75 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_76 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_77 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_78 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_79 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_80 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_81 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_82 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_83 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_84 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_85 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_86 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_87 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_88 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_89 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_90 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_91 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_92 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_93 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_94 | 1.0 | 0.0 | 1.0 |
| DMACJMHG_95 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_96 | 1.0 | 0.0 | 1.0 |
| DMACJMHG_97 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_98 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_99 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_100 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_101 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_102 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_103 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_104 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_105 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_106 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_107 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_108 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_109 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_110 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_111 | 1.0 | 0.0 | 1.0 |
| DMACJMHG_112 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_113 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_114 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_115 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_116 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_117 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_118 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_119 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_120 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_121 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_122 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_123 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_124 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_125 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_126 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_127 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_128 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_129 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_130 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_131 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_132 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_133 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_134 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_135 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_136 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_137 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_138 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_139 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_140 | 0.0 | 1.0 | 0.0 |
| DMACJMHG_141 | 1.0 | 0.0 | 1.0 |
| DMACJMHG_142 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DMACJMHG_24 | 6761 | 25006 | False | Myoviridae | VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| DMACJMHG_56 | 51514 | 73096 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227 |
Phigaro v2.4.0 (Database: Jan. 2024 release)