Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 8 | 0 | 1 | 8.06 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ENHKNMLC_7 | 695765 | 696655 | CblA-1 | ARO:3002999 | CblA-1 | 99.32 | 100.00 | GQ343019.1 |
| ENHKNMLC_20 | 159369 | 160232 | aadS | ARO:3004683 | aadS | 100.00 | 100.00 | M72415.1 |
| ENHKNMLC_20 | 158052 | 159188 | tet(X4) | ARO:3004720 | tet(X4) | 92.59 | 98.18 | MK134376.1 |
| ENHKNMLC_20 | 157047 | 157871 | OXA-347 | ARO:3001777 | OXA-347 | 99.27 | 100.00 | JN086160.1 |
| ENHKNMLC_20 | 156044 | 156844 | ErmF | ARO:3000498 | ErmF | 99.62 | 100.00 | M17124.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ENHKNMLC_20 | 156044 | 156844 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | M17808 |
| ENHKNMLC_20 | 157047 | 157871 | blaOXA-347 | Unknown Beta-lactam | 99.64 | 100.00 | ACWG01000053 |
| ENHKNMLC_20 | 158072 | 159188 | tet(X6) | Doxycycline, Tetracycline, Minocycline, Tigecycline | 97.23 | 98.07 | MN507533 |
| ENHKNMLC_15 | 28275 | 29029 | cfxA6 | Unknown Beta-lactam | 87.43 | 75.70 | GQ342996 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ENHKNMLC_20 | 159372 | 160232 | aadS | aminoglycoside 6-adenylyltransferase AadS | EXACTX | 100.00 | 100.00 | WP_003013318.1 |
| ENHKNMLC_20 | 156044 | 156841 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | EXACTX | 100.00 | 100.00 | WP_002682030.1 |
| ENHKNMLC_7 | 695765 | 696652 | cblA | CblA family class A beta-lactamase | BLASTX | 99.32 | 100.00 | WP_005827792.1 |
| ENHKNMLC_20 | 157050 | 157871 | blaOXA | class D beta-lactamase | BLASTX | 99.27 | 100.00 | WP_004295324.1 |
| ENHKNMLC_20 | 160411 | 161250 | estT | macrolide hydrolase EstT | BLASTX | 98.94 | 100.00 | WP_185148407.1 |
| ENHKNMLC_20 | 158058 | 159218 | tet(X5) | tetracycline-inactivating monooxygenase Tet(X5) | BLASTX | 93.28 | 99.74 | WP_150378267.1 |
| ENHKNMLC_15 | 28226 | 29170 | cfxA6 | broad-spectrum class A beta-lactamase CfxA6 | BLASTX | 80.32 | 98.44 | WP_117664257.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ENHKNMLC_3 | 383 | 1963 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 80.64 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ENHKNMLC_1 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_2 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_3 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_4 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_5 | 1.0 | 0.0 | 1.0 |
| ENHKNMLC_6 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_7 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_8 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_9 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_10 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_11 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_13 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_14 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_15 | 1.0 | 0.0 | 1.0 |
| ENHKNMLC_16 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_17 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_18 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_19 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_20 | 0.0 | 1.0 | 0.0 |
| ENHKNMLC_21 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ENHKNMLC_18 | 631441 | 645466 | False | Unknown | VOG0083, |
Phigaro v2.4.0 (Database: Jan. 2024 release)