Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 34 | 41.05 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KFKLDJKC_4 | 32443 | 33701 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 96.67 | 99.92 | WP_011681445 |
| KFKLDJKC_4 | 34748 | 35463 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 95.81 | 99.86 | WP_011681442 |
| KFKLDJKC_4 | 31473 | 32324 | rfbD | 0.0 | Capsule | Immune modulation | 94.95 | 100.00 | WP_014621820 |
| KFKLDJKC_42 | 1754 | 2485 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.67 | 100.00 | WP_011227248 |
| KFKLDJKC_4 | 38790 | 39596 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 94.18 | 100.00 | WP_011681437 |
| KFKLDJKC_4 | 38790 | 39596 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 94.18 | 100.00 | WP_011227429 |
| KFKLDJKC_42 | 1754 | 2485 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.99 | 100.00 | WP_011226048 |
| KFKLDJKC_42 | 1754 | 2485 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.72 | 100.00 | WP_011681176 |
| KFKLDJKC_4 | 35809 | 36585 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.67 | 99.87 | WP_011681440 |
| KFKLDJKC_4 | 39596 | 40798 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 92.27 | 100.00 | WP_011681436 |
| KFKLDJKC_4 | 39596 | 40798 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 92.02 | 100.00 | WP_011227428 |
| KFKLDJKC_42 | 293 | 1753 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 91.33 | 99.86 | WP_011681177 |
| KFKLDJKC_22 | 93770 | 94363 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.25 | 100.00 | WP_011226149 |
| KFKLDJKC_22 | 93770 | 94363 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| KFKLDJKC_4 | 37849 | 38790 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011681438 |
| KFKLDJKC_22 | 93770 | 94363 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| KFKLDJKC_35 | 14137 | 15795 | fbp54 | 0.0 | FBPs | Adherence | 91.02 | 99.94 | WP_011227179 |
| KFKLDJKC_4 | 37849 | 38790 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227430 |
| KFKLDJKC_42 | 293 | 1753 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.72 | 100.00 | WP_011226049 |
| KFKLDJKC_4 | 40816 | 42401 | STER_RS07050 | 0.0 | Capsule | Immune modulation | 90.50 | 93.06 | WP_041827753 |
| KFKLDJKC_4 | 40816 | 42401 | STR_RS06890 | 0.0 | Capsule | Immune modulation | 90.25 | 93.06 | WP_041827077 |
| KFKLDJKC_42 | 293 | 1753 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 90.03 | 100.00 | WP_041827043 |
| KFKLDJKC_59 | 20387 | 21292 | cbpD | 0.0 | Autolysin | Exoenzyme | 89.96 | 93.50 | WP_082302295 |
| KFKLDJKC_42 | 2494 | 3186 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| KFKLDJKC_42 | 2494 | 3186 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| KFKLDJKC_42 | 2494 | 3186 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| KFKLDJKC_4 | 36704 | 37852 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 89.21 | 100.00 | WP_011227431 |
| KFKLDJKC_4 | 36704 | 37852 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 89.03 | 100.00 | WP_011681439 |
| KFKLDJKC_10 | 30754 | 32057 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| KFKLDJKC_4 | 42476 | 44221 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 88.06 | 99.77 | WP_011227426 |
| KFKLDJKC_4 | 42476 | 44221 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 87.94 | 99.77 | WP_011681434 |
| KFKLDJKC_18 | 13432 | 14442 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.18 | 99.80 | WP_002986042 |
| KFKLDJKC_4 | 38790 | 39596 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 84.63 | 100.00 | WP_012000440 |
| KFKLDJKC_16 | 22323 | 23084 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| KFKLDJKC_22 | 94633 | 95679 | rfbB | 0.0 | Capsule | Immune modulation | 83.40 | 99.90 | WP_002263087 |
| KFKLDJKC_55 | 98032 | 99315 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.92 | 99.61 | WP_000107753 |
| KFKLDJKC_22 | 92901 | 93770 | rfbA | 0.0 | Capsule | Immune modulation | 80.28 | 99.77 | WP_002904719 |
| KFKLDJKC_42 | 3196 | 3866 | STU_RS14635 | 2.60e-148 | Capsule | Immune modulation | 80.98 | 90.28 | WP_011226046 |
| KFKLDJKC_42 | 3196 | 3866 | STER_RS05305 | 1.21e-146 | Capsule | Immune modulation | 80.83 | 90.28 | WP_011681174 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KFKLDJKC_16 | 25774 | 26757 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| KFKLDJKC_16 | 14184 | 13111 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| KFKLDJKC_39 | 2403 | 97 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| KFKLDJKC_28 | 5512 | 7758 | metE | 0.0 | methionine biosynthesis enzymes | 92.66 | 100.00 | AAK74738 |
| KFKLDJKC_39 | 203408 | 204376 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| KFKLDJKC_4 | 257424 | 255943 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.51 | 100.00 | ABJ54480 |
| KFKLDJKC_36 | 37888 | 36917 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| KFKLDJKC_4 | 339054 | 345617 | PrtA | 0.0 | serine protease | 90.14 | 101.96 | ABJ55268 |
| KFKLDJKC_22 | 67522 | 69252 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.78 | 100.00 | AAK34199 |
| KFKLDJKC_39 | 126382 | 127533 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| KFKLDJKC_4 | 19203 | 21191 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| KFKLDJKC_24 | 40172 | 38694 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| KFKLDJKC_10 | 30754 | 32055 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| KFKLDJKC_24 | 24842 | 22950 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| KFKLDJKC_30 | 6870 | 8579 | PGM | 0.0 | phosphoglucomutase | 84.59 | 99.65 | ACH87041 |
| KFKLDJKC_36 | 1979 | 888 | recA | 0.0 | DNA recombination and repair | 84.37 | 95.36 | AAL00560 |
| KFKLDJKC_35 | 208 | 1569 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.89 | 99.56 | ABP90420 |
| KFKLDJKC_9 | 19791 | 20801 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| KFKLDJKC_10 | 16344 | 17234 | bglB | 1.18e-177 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| KFKLDJKC_23 | 48054 | 49004 | SP_0676 | 1.30e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| KFKLDJKC_28 | 7948 | 8805 | metF | 3.56e-170 | methionine biosynthesis enzymes | 87.06 | 99.31 | AAK74739 |
| KFKLDJKC_57 | 71484 | 72188 | covR | 3.96e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| KFKLDJKC_30 | 2204 | 1533 | ciaR | 7.99e-127 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| KFKLDJKC_4 | 321185 | 321856 | rr01 | 8.87e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| KFKLDJKC_30 | 2204 | 1536 | CiaR | 1.48e-126 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| KFKLDJKC_27 | 50509 | 51195 | CovR | 8.72e-121 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| KFKLDJKC_57 | 71484 | 72188 | walR | 9.00e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| KFKLDJKC_36 | 713 | 318 | SpxA2 | 3.75e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| KFKLDJKC_52 | 11421 | 11822 | SpxA1 | 2.98e-70 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| KFKLDJKC_25 | 5536 | 5949 | GrpE | 4.51e-68 | protein grpE | 81.16 | 80.59 | ABP89267 |
| KFKLDJKC_27 | 73712 | 73999 | spr0479 | 2.75e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| KFKLDJKC_39 | 168525 | 168779 | spr1327 | 1.50e-44 | essential gene | 91.77 | 100.00 | AAL00131 |
| KFKLDJKC_9 | 110583 | 110846 | spr0175 | 7.00e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| KFKLDJKC_9 | 111408 | 111674 | spr0177 | 1.56e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| KFKLDJKC_55 | 97865 | 97374 | RopE (M5005_Spy_1611) | 4.88e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| KFKLDJKC_39 | 2403 | 103 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| KFKLDJKC_9 | 55925 | 54906 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KFKLDJKC_4 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_5 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_7 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_9 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_10 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_11 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_12 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_14 | 1.0 | 0.0 | 1.0 |
| KFKLDJKC_15 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_16 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_17 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_18 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_20 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_21 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_22 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_23 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_24 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_25 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_26 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_27 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_28 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_29 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_30 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_35 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_36 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_38 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_39 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_42 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_45 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_47 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_48 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_49 | 1.0 | 0.0 | 1.0 |
| KFKLDJKC_50 | 1.0 | 0.0 | 1.0 |
| KFKLDJKC_52 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_54 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_55 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_57 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_58 | 0.0 | 1.0 | 0.0 |
| KFKLDJKC_59 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KFKLDJKC_10 | 34645 | 76958 | False | Siphoviridae | VOG0743, VOG3622, VOG1300, VOG6425, VOG6915, VOG1564, VOG9469, VOG4555, VOG0651, VOG0321, VOG0322, VOG4548, VOG6545, VOG4632, VOG4606, VOG0327, VOG6414, VOG0656, VOG7484, VOG6414, VOG0657, VOG0044, VOG0658, VOG0198, VOG0796, VOG4544, VOG0720, VOG0720, VOG4564, VOG3434, VOG1887, VOG3435, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG3636, VOG4605, VOG4599, VOG0647, VOG0648, VOG0641, VOG0054, VOG4649, VOG3299, VOG8184 |
Phigaro v2.4.0 (Database: Jan. 2024 release)