Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 6 | 6.63 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KPJJHKEO_24 | 59709 | 60758 | clbA | ARO:3002814 | clbA | 97.42 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KPJJHKEO_24 | 59723 | 60758 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.03 | 98.67 | KR610408 |
| KPJJHKEO_12 | 377566 | 378942 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.36 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KPJJHKEO_24 | 59715 | 60758 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 97.70 | 99.71 | WP_012116915.1 |
| KPJJHKEO_12 | 377566 | 378936 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KPJJHKEO_22 | 79381 | 80562 | capB | 0.0 | Capsule | Immune modulation | 82.38 | 99.66 | NP_391471 |
| KPJJHKEO_22 | 78917 | 79366 | capC | 1.02e-112 | Capsule | Immune modulation | 83.37 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KPJJHKEO_27 | 31644 | 32780 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| KPJJHKEO_2 | 12449 | 11580 | pdxS | 2.57e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| KPJJHKEO_1 | 341719 | 340943 | CodY | 1.30e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KPJJHKEO_1 | 836717 | 836325 | spxA1 | 2.37e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| KPJJHKEO_24 | 91531 | 91334 | CspA | 2.45e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| KPJJHKEO_24 | 91531 | 91337 | CspR | 3.20e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| KPJJHKEO_23 | 138039 | 137167 | lipA (SAUSA300_0829) | 1.37e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KPJJHKEO_1 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_2 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_3 | 1.0 | 0.0 | 1.0 |
| KPJJHKEO_4 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_5 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_8 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_9 | 1.0 | 0.0 | 1.0 |
| KPJJHKEO_10 | 1.0 | 0.0 | 1.0 |
| KPJJHKEO_11 | 1.0 | 0.0 | 1.0 |
| KPJJHKEO_12 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_22 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_23 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_24 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_25 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_26 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_27 | 0.0 | 1.0 | 0.0 |
| KPJJHKEO_28 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KPJJHKEO_1 | 724990 | 751448 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
| KPJJHKEO_12 | 395074 | 430119 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9667, VOG4673, VOG4662, VOG0796, VOG10227, VOG4773, VOG4688, VOG5853, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG10989, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)