Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 7 | 16.43 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GEHLFIEJ_4 | 719161 | 719499 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| GEHLFIEJ_4 | 718844 | 719161 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| GEHLFIEJ_6 | 637173 | 638342 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| GEHLFIEJ_6 | 467023 | 468225 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| GEHLFIEJ_6 | 437886 | 438740 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| GEHLFIEJ_7 | 351801 | 352721 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| GEHLFIEJ_7 | 338471 | 339910 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| GEHLFIEJ_7 | 288795 | 289388 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| GEHLFIEJ_8 | 28965 | 31535 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| GEHLFIEJ_7 | 28777 | 30423 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GEHLFIEJ_6 | 437886 | 438740 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| GEHLFIEJ_7 | 351801 | 352721 | mph(K) | Spiramycin, Telithromycin | 97.18 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GEHLFIEJ_7 | 28777 | 30420 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| GEHLFIEJ_6 | 437886 | 438737 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| GEHLFIEJ_7 | 351804 | 352721 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| GEHLFIEJ_6 | 932970 | 935567 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GEHLFIEJ_5 | 529851 | 531032 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| GEHLFIEJ_5 | 956610 | 957395 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| GEHLFIEJ_5 | 531047 | 531496 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| GEHLFIEJ_6 | 837261 | 837902 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| GEHLFIEJ_5 | 531515 | 532657 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| GEHLFIEJ_5 | 957421 | 958617 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| GEHLFIEJ_6 | 983167 | 984930 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| GEHLFIEJ_5 | 961251 | 968387 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.77 | 100.00 | NP_391076 |
| GEHLFIEJ_5 | 958646 | 960265 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.46 | 99.94 | NP_391078 |
| GEHLFIEJ_5 | 960293 | 961231 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.30 | 100.00 | NP_391077 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GEHLFIEJ_9 | 31737 | 32873 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| GEHLFIEJ_11 | 4306 | 5175 | pdxS | 4.74e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| GEHLFIEJ_4 | 407289 | 406513 | CodY | 9.27e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GEHLFIEJ_5 | 687506 | 686937 | ClpP | 6.44e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| GEHLFIEJ_4 | 867949 | 867557 | spxA1 | 7.28e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| GEHLFIEJ_4 | 867949 | 867557 | spx | 4.35e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| GEHLFIEJ_10 | 34706 | 34464 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| GEHLFIEJ_7 | 94905 | 94708 | CspA | 1.91e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GEHLFIEJ_4 | 0.0 | 1.0 | 0.0 |
| GEHLFIEJ_5 | 0.0 | 1.0 | 0.0 |
| GEHLFIEJ_6 | 0.0 | 1.0 | 0.0 |
| GEHLFIEJ_7 | 0.0 | 1.0 | 0.0 |
| GEHLFIEJ_8 | 0.0 | 1.0 | 0.0 |
| GEHLFIEJ_9 | 0.0 | 1.0 | 0.0 |
| GEHLFIEJ_10 | 0.0 | 1.0 | 0.0 |
| GEHLFIEJ_11 | 0.0 | 1.0 | 0.0 |
| GEHLFIEJ_12 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GEHLFIEJ_4 | 177809 | 186022 | False | Myoviridae | VOG5722, VOG5779, VOG5721, VOG10940, VOG7134, VOG9867 |
| GEHLFIEJ_4 | 189874 | 209191 | False | Siphoviridae | VOG7718, VOG3390, VOG7895, VOG8610, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712 |
| GEHLFIEJ_4 | 749024 | 775778 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)