Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 7 | 17.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FKNIGBGJ_2 | 1643882 | 1644220 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| FKNIGBGJ_2 | 1643565 | 1643882 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| FKNIGBGJ_2 | 655069 | 656238 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FKNIGBGJ_2 | 465122 | 466324 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| FKNIGBGJ_2 | 438433 | 439287 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| FKNIGBGJ_4 | 370241 | 371161 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
| FKNIGBGJ_4 | 356917 | 358350 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| FKNIGBGJ_4 | 307295 | 307888 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| FKNIGBGJ_5 | 256662 | 259232 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| FKNIGBGJ_4 | 31378 | 33024 | vmlR | ARO:3004476 | vmlR | 97.45 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FKNIGBGJ_2 | 438433 | 439287 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
| FKNIGBGJ_4 | 370241 | 371161 | mph(K) | Spiramycin, Telithromycin | 97.39 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FKNIGBGJ_2 | 438433 | 439284 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| FKNIGBGJ_4 | 370244 | 371161 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| FKNIGBGJ_4 | 31378 | 33021 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.45 | 100.00 | WP_003234144.1 |
| FKNIGBGJ_3 | 42411 | 42929 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.80 | 100.00 | WP_003242546.1 |
| FKNIGBGJ_2 | 960148 | 962745 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FKNIGBGJ_3 | 535345 | 536526 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| FKNIGBGJ_3 | 943927 | 944712 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| FKNIGBGJ_2 | 855053 | 855694 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| FKNIGBGJ_3 | 537009 | 538151 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| FKNIGBGJ_3 | 536541 | 536990 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| FKNIGBGJ_3 | 944738 | 945934 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| FKNIGBGJ_3 | 948568 | 955704 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.61 | 100.00 | NP_391076 |
| FKNIGBGJ_2 | 1014151 | 1015914 | dep/capD | 0.0 | Capsule | Immune modulation | 98.53 | 100.00 | NP_389723 |
| FKNIGBGJ_3 | 947610 | 948548 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| FKNIGBGJ_3 | 945963 | 947582 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.27 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FKNIGBGJ_6 | 31717 | 32853 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| FKNIGBGJ_8 | 11247 | 10378 | pdxS | 1.55e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| FKNIGBGJ_2 | 1332002 | 1331226 | CodY | 1.68e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FKNIGBGJ_3 | 693021 | 692452 | ClpP | 6.36e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FKNIGBGJ_2 | 1791590 | 1791198 | spxA1 | 1.32e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| FKNIGBGJ_2 | 1791590 | 1791198 | spx | 7.85e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| FKNIGBGJ_7 | 20589 | 20831 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FKNIGBGJ_4 | 93007 | 92810 | CspA | 1.99e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FKNIGBGJ_2 | 0.0 | 1.0 | 0.0 |
| FKNIGBGJ_3 | 0.0 | 1.0 | 0.0 |
| FKNIGBGJ_4 | 0.0 | 1.0 | 0.0 |
| FKNIGBGJ_5 | 0.0 | 1.0 | 0.0 |
| FKNIGBGJ_6 | 0.0 | 1.0 | 0.0 |
| FKNIGBGJ_7 | 0.0 | 1.0 | 0.0 |
| FKNIGBGJ_8 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FKNIGBGJ_2 | 1673443 | 1701172 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)