Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GFOAJMHB_5 | 504830 | 505168 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| GFOAJMHB_5 | 504513 | 504830 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| GFOAJMHB_8 | 345826 | 346746 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| GFOAJMHB_8 | 332491 | 333930 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| GFOAJMHB_9 | 283147 | 285717 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| GFOAJMHB_8 | 282779 | 283372 | tmrB | ARO:3003059 | tmrB | 98.98 | 100.00 | AL009126.3 |
| GFOAJMHB_6 | 267350 | 268204 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| GFOAJMHB_6 | 250553 | 251755 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| GFOAJMHB_8 | 28957 | 30600 | vmlR | ARO:3004476 | vmlR | 98.72 | 100.00 | NC_000964.3 |
| GFOAJMHB_6 | 20440 | 21609 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GFOAJMHB_4 | 36375 | 37751 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.69 | 100.00 | X08034 |
| GFOAJMHB_6 | 267352 | 268204 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| GFOAJMHB_8 | 345826 | 346746 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GFOAJMHB_8 | 28957 | 30597 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.72 | 100.00 | WP_003234144.1 |
| GFOAJMHB_4 | 36375 | 37748 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| GFOAJMHB_8 | 345829 | 346746 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| GFOAJMHB_6 | 267353 | 268204 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| GFOAJMHB_4 | 39704 | 40222 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| GFOAJMHB_7 | 288914 | 291511 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GFOAJMHB_7 | 192292 | 192933 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| GFOAJMHB_4 | 513929 | 515110 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| GFOAJMHB_4 | 515593 | 516735 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| GFOAJMHB_4 | 932635 | 933420 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| GFOAJMHB_4 | 515125 | 515574 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| GFOAJMHB_4 | 936318 | 937256 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| GFOAJMHB_4 | 933446 | 934642 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| GFOAJMHB_4 | 937276 | 944412 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.09 | 100.00 | NP_391076 |
| GFOAJMHB_4 | 934671 | 936290 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.07 | 100.00 | NP_391078 |
| GFOAJMHB_7 | 336608 | 338371 | dep/capD | 0.0 | Capsule | Immune modulation | 98.70 | 100.00 | NP_389723 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GFOAJMHB_10 | 27998 | 26862 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| GFOAJMHB_12 | 4308 | 5177 | pdxS | 2.57e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| GFOAJMHB_5 | 194235 | 193459 | CodY | 7.55e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GFOAJMHB_4 | 677550 | 676981 | ClpP | 2.13e-98 | part of proteolytic complex | 80.53 | 97.44 | BAB94595 |
| GFOAJMHB_5 | 655299 | 654907 | spxA1 | 5.94e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| GFOAJMHB_5 | 655299 | 654907 | spx | 3.54e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| GFOAJMHB_11 | 20577 | 20819 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| GFOAJMHB_8 | 81817 | 81620 | CspA | 1.89e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| GFOAJMHB_7 | 180329 | 180135 | CspD | 2.89e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GFOAJMHB_1 | 1.0 | 0.0 | 1.0 |
| GFOAJMHB_4 | 0.0 | 1.0 | 0.0 |
| GFOAJMHB_5 | 0.0 | 1.0 | 0.0 |
| GFOAJMHB_6 | 0.0 | 1.0 | 0.0 |
| GFOAJMHB_7 | 0.0 | 1.0 | 0.0 |
| GFOAJMHB_8 | 0.0 | 1.0 | 0.0 |
| GFOAJMHB_9 | 0.0 | 1.0 | 0.0 |
| GFOAJMHB_10 | 0.0 | 1.0 | 0.0 |
| GFOAJMHB_11 | 0.0 | 1.0 | 0.0 |
| GFOAJMHB_12 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GFOAJMHB_5 | 534658 | 561780 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| GFOAJMHB_6 | 199491 | 222049 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773 |
Phigaro v2.4.0 (Database: Jan. 2024 release)