Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 33 | 40.22 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HOCMNKEB_37 | 179012 | 179881 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| HOCMNKEB_37 | 178419 | 179012 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| HOCMNKEB_11 | 12972 | 14630 | fbp54 | 0.0 | FBPs | Adherence | 99.94 | 100.00 | WP_011227179 |
| HOCMNKEB_37 | 178419 | 179012 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| HOCMNKEB_20 | 96516 | 97775 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 99.76 | 100.00 | WP_011681445 |
| HOCMNKEB_37 | 178419 | 179012 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| HOCMNKEB_12 | 1362 | 2201 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| HOCMNKEB_20 | 95543 | 96519 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.39 | 99.90 | WP_011681444 |
| HOCMNKEB_15 | 52160 | 52950 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.24 | 100.00 | - |
| HOCMNKEB_20 | 94557 | 95525 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.17 | 100.00 | WP_011681443 |
| HOCMNKEB_14 | 5055 | 5786 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011681176 |
| HOCMNKEB_30 | 3373 | 4788 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.38 | 100.00 | WP_011681165 |
| HOCMNKEB_14 | 5787 | 7241 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.35 | 100.00 | WP_041827043 |
| HOCMNKEB_14 | 5055 | 5786 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011227248 |
| HOCMNKEB_20 | 93840 | 94555 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.05 | 99.86 | WP_011681442 |
| HOCMNKEB_14 | 5787 | 7241 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.01 | 100.00 | WP_011226049 |
| HOCMNKEB_14 | 5055 | 5786 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.95 | 100.00 | WP_011226048 |
| HOCMNKEB_14 | 3604 | 4344 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011226046 |
| HOCMNKEB_14 | 4354 | 5046 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011227247 |
| HOCMNKEB_14 | 4354 | 5046 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011226047 |
| HOCMNKEB_16 | 14648 | 15409 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| HOCMNKEB_14 | 3604 | 4344 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.57 | 100.00 | WP_011681174 |
| HOCMNKEB_14 | 3609 | 4344 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.28 | 98.13 | WP_011227246 |
| HOCMNKEB_20 | 92724 | 93494 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.15 | 99.10 | WP_011681440 |
| HOCMNKEB_14 | 5787 | 7241 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.12 | 99.79 | WP_011681177 |
| HOCMNKEB_20 | 97894 | 98745 | rfbD | 0.0 | Capsule | Immune modulation | 96.36 | 100.00 | WP_014621820 |
| HOCMNKEB_14 | 2180 | 3547 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.32 | 100.00 | WP_011226045 |
| HOCMNKEB_14 | 2182 | 3547 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.27 | 99.85 | WP_011681173 |
| HOCMNKEB_14 | 4354 | 5046 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 91.34 | 100.00 | WP_011681175 |
| HOCMNKEB_41 | 97889 | 99192 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| HOCMNKEB_25 | 9178 | 10188 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.55 | 99.90 | WP_002986042 |
| HOCMNKEB_37 | 7466 | 8749 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| HOCMNKEB_37 | 176944 | 177990 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| HOCMNKEB_30 | 1005 | 1591 | SPD_RS01775 | 5.80e-144 | Capsule | Immune modulation | 82.48 | 96.70 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HOCMNKEB_16 | 18096 | 19079 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| HOCMNKEB_4 | 40225 | 41298 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| HOCMNKEB_21 | 137790 | 140093 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| HOCMNKEB_41 | 14213 | 15181 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| HOCMNKEB_18 | 39863 | 41344 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| HOCMNKEB_5 | 5519 | 7765 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| HOCMNKEB_12 | 33578 | 34570 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| HOCMNKEB_1 | 4625 | 5596 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| HOCMNKEB_21 | 24119 | 22968 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| HOCMNKEB_20 | 111994 | 110006 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| HOCMNKEB_16 | 1901 | 171 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| HOCMNKEB_29 | 101285 | 99807 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| HOCMNKEB_41 | 97889 | 99190 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| HOCMNKEB_29 | 86097 | 84205 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| HOCMNKEB_5 | 10690 | 8981 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| HOCMNKEB_27 | 20110 | 21120 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| HOCMNKEB_13 | 19816 | 18455 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| HOCMNKEB_28 | 27171 | 25279 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| HOCMNKEB_41 | 83454 | 84344 | bglB | 7.10e-180 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| HOCMNKEB_9 | 11847 | 10912 | SP_0676 | 1.73e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| HOCMNKEB_5 | 7957 | 8814 | metF | 4.10e-166 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| HOCMNKEB_15 | 24473 | 23769 | covR | 1.83e-131 | responder protein | 84.68 | 100.00 | AAK71319 |
| HOCMNKEB_18 | 11769 | 11098 | rr01 | 8.53e-126 | response regulator | 83.93 | 99.56 | CAB54566 |
| HOCMNKEB_15 | 24473 | 23769 | walR | 1.31e-121 | DNA-binding response regulator | 80.85 | 100.00 | ANC99504 |
| HOCMNKEB_5 | 14647 | 15315 | CiaR | 1.80e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| HOCMNKEB_37 | 160518 | 161204 | CovR | 1.08e-118 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| HOCMNKEB_5 | 14647 | 15318 | ciaR | 1.77e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| HOCMNKEB_12 | 34882 | 35277 | SpxA2 | 7.06e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| HOCMNKEB_13 | 31165 | 31566 | SpxA1 | 1.74e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| HOCMNKEB_38 | 13229 | 13516 | spr0479 | 2.96e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| HOCMNKEB_39 | 133508 | 133762 | spr1327 | 2.36e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| HOCMNKEB_29 | 43519 | 43782 | spr0175 | 1.91e-41 | pathogenitcity related | 82.95 | 100.00 | AAK98979 |
| HOCMNKEB_29 | 44322 | 44612 | spr0177 | 3.64e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| HOCMNKEB_37 | 8907 | 9407 | RopE (M5005_Spy_1611) | 5.29e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| HOCMNKEB_21 | 137790 | 140087 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| HOCMNKEB_28 | 12767 | 11748 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 95.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HOCMNKEB_1 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_2 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_3 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_4 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_5 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_6 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_7 | 1.0 | 0.0 | 1.0 |
| HOCMNKEB_8 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_9 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_10 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_11 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_12 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_13 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_14 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_15 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_16 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_17 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_18 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_19 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_20 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_21 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_22 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_23 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_24 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_25 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_26 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_27 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_28 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_29 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_30 | 1.0 | 0.0 | 1.0 |
| HOCMNKEB_32 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_33 | 1.0 | 0.0 | 1.0 |
| HOCMNKEB_34 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_35 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_36 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_37 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_38 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_39 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_40 | 0.0 | 1.0 | 0.0 |
| HOCMNKEB_41 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)