Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 33 | 39.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| CCFLLOKH_24 | 2200 | 3069 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| CCFLLOKH_24 | 1607 | 2200 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| CCFLLOKH_24 | 1607 | 2200 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| CCFLLOKH_35 | 19421 | 20680 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 99.76 | 100.00 | WP_011681445 |
| CCFLLOKH_24 | 1607 | 2200 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| CCFLLOKH_18 | 4599 | 6257 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| CCFLLOKH_12 | 20758 | 21597 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| CCFLLOKH_35 | 18448 | 19424 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.39 | 99.90 | WP_011681444 |
| CCFLLOKH_21 | 85645 | 86435 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.24 | 100.00 | - |
| CCFLLOKH_35 | 17462 | 18430 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.17 | 100.00 | WP_011681443 |
| CCFLLOKH_26 | 14653 | 15414 | srtA | 0.0 | Sortase A | Exoenzyme | 99.08 | 100.00 | - |
| CCFLLOKH_21 | 18940 | 19671 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| CCFLLOKH_21 | 8953 | 10368 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | WP_011681165 |
| CCFLLOKH_21 | 18940 | 19671 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011226048 |
| CCFLLOKH_21 | 18940 | 19671 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011227248 |
| CCFLLOKH_35 | 16745 | 17460 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.05 | 99.86 | WP_011681442 |
| CCFLLOKH_21 | 18239 | 18931 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.83 | 100.00 | WP_011227247 |
| CCFLLOKH_21 | 18239 | 18931 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.83 | 100.00 | WP_011226047 |
| CCFLLOKH_21 | 19672 | 21132 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011681177 |
| CCFLLOKH_35 | 15629 | 16399 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.15 | 99.10 | WP_011681440 |
| CCFLLOKH_21 | 17491 | 18229 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.02 | 99.73 | WP_011681174 |
| CCFLLOKH_21 | 19672 | 21132 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.92 | 100.21 | WP_011226049 |
| CCFLLOKH_21 | 17494 | 18229 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.47 | 98.13 | WP_011227246 |
| CCFLLOKH_35 | 20799 | 21650 | rfbD | 0.0 | Capsule | Immune modulation | 96.36 | 100.00 | WP_014621820 |
| CCFLLOKH_21 | 17491 | 18229 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.35 | 99.73 | WP_011226046 |
| CCFLLOKH_21 | 19672 | 21132 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.96 | 100.21 | WP_041827043 |
| CCFLLOKH_21 | 16064 | 17431 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.96 | 100.00 | WP_011226045 |
| CCFLLOKH_21 | 16066 | 17431 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.36 | 99.85 | WP_011681173 |
| CCFLLOKH_21 | 18239 | 18931 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.04 | 100.00 | WP_011681175 |
| CCFLLOKH_4 | 30764 | 32067 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| CCFLLOKH_42 | 474 | 1484 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.86 | 99.90 | WP_002986042 |
| CCFLLOKH_23 | 3021 | 4304 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| CCFLLOKH_24 | 132 | 1178 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| CCFLLOKH_26 | 18101 | 19084 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| CCFLLOKH_7 | 27708 | 28781 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| CCFLLOKH_3 | 53343 | 54311 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| CCFLLOKH_40 | 22954 | 25257 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| CCFLLOKH_28 | 12181 | 13662 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| CCFLLOKH_10 | 8741 | 10987 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| CCFLLOKH_12 | 41260 | 42252 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| CCFLLOKH_12 | 4276 | 5247 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| CCFLLOKH_37 | 19750 | 18599 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| CCFLLOKH_35 | 34900 | 32912 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| CCFLLOKH_26 | 1901 | 171 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| CCFLLOKH_48 | 31541 | 30063 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| CCFLLOKH_4 | 30764 | 32065 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| CCFLLOKH_48 | 16352 | 14460 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| CCFLLOKH_10 | 13912 | 12203 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| CCFLLOKH_43 | 21652 | 22662 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| CCFLLOKH_19 | 1793 | 432 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| CCFLLOKH_44 | 15571 | 13679 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| CCFLLOKH_4 | 16334 | 17224 | bglB | 5.80e-180 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| CCFLLOKH_16 | 9971 | 9033 | SP_0676 | 2.90e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| CCFLLOKH_10 | 11179 | 12036 | metF | 4.91e-166 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| CCFLLOKH_21 | 57965 | 57261 | covR | 3.08e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| CCFLLOKH_26 | 65740 | 65069 | rr01 | 1.59e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| CCFLLOKH_21 | 57965 | 57261 | walR | 1.30e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| CCFLLOKH_55 | 29793 | 30479 | CovR | 2.03e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| CCFLLOKH_10 | 17869 | 18537 | CiaR | 2.15e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| CCFLLOKH_10 | 17869 | 18540 | ciaR | 2.11e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| CCFLLOKH_12 | 42564 | 42959 | SpxA2 | 8.60e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| CCFLLOKH_19 | 13150 | 13551 | SpxA1 | 1.28e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| CCFLLOKH_56 | 13241 | 13528 | spr0479 | 2.96e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| CCFLLOKH_3 | 18930 | 19184 | spr1327 | 1.57e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| CCFLLOKH_47 | 15813 | 16076 | spr0175 | 1.28e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| CCFLLOKH_47 | 16640 | 16906 | spr0177 | 2.81e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| CCFLLOKH_23 | 4462 | 4962 | RopE (M5005_Spy_1611) | 7.94e-77 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| CCFLLOKH_40 | 22954 | 25251 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| CCFLLOKH_44 | 1167 | 148 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 95.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| CCFLLOKH_1 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_2 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_3 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_4 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_5 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_6 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_7 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_8 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_9 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_10 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_11 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_12 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_13 | 1.0 | 0.0 | 1.0 |
| CCFLLOKH_14 | 1.0 | 0.0 | 1.0 |
| CCFLLOKH_15 | 1.0 | 0.0 | 1.0 |
| CCFLLOKH_16 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_17 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_18 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_19 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_20 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_21 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_22 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_23 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_24 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_25 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_26 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_27 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_28 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_29 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_30 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_31 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_32 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_33 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_34 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_35 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_36 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_37 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_38 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_39 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_40 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_41 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_42 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_43 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_44 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_45 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_46 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_47 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_48 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_50 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_51 | 1.0 | 0.0 | 1.0 |
| CCFLLOKH_52 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_53 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_54 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_55 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_56 | 0.0 | 1.0 | 0.0 |
| CCFLLOKH_57 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)