safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	28	33	43.28

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
KIKOJKAI_8	139853	140722	rfbA	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681286
KIKOJKAI_8	139260	139853	STER_RS06030	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
KIKOJKAI_10	17007	18752	STER_RS07045	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681434
KIKOJKAI_10	18754	20454	STER_RS07050	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827753
KIKOJKAI_10	20472	21674	STER_RS07055	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681436
KIKOJKAI_10	21674	22480	STER_RS07060	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681437
KIKOJKAI_10	22480	23421	STER_RS07065	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681438
KIKOJKAI_10	23418	24566	STER_RS07070	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681439
KIKOJKAI_10	24685	25461	STER_RS07075	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681440
KIKOJKAI_10	25807	26523	STER_RS07085	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681442
KIKOJKAI_10	26524	27492	STER_RS07090	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681443
KIKOJKAI_10	28484	29743	STER_RS07100	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681445
KIKOJKAI_10	27510	28487	STER_RS07095	0.0	Capsule	Immune modulation	99.90	100.00	WP_011681444
KIKOJKAI_8	139260	139853	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
KIKOJKAI_10	18754	20454	STR_RS06890	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827077
KIKOJKAI_8	139260	139853	STU_RS15260	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
KIKOJKAI_6	65617	67275	fbp54	0.0	FBPs	Adherence	99.58	100.00	WP_011227179
KIKOJKAI_1	20790	21629	cbpD	0.0	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
KIKOJKAI_10	21674	22480	STR_RS06900	0.0	Capsule	Immune modulation	99.50	100.00	WP_011227429
KIKOJKAI_28	1117	2260	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.48	100.00	-
KIKOJKAI_10	22480	23421	STR_RS06905	0.0	Capsule	Immune modulation	99.47	100.00	WP_011227430
KIKOJKAI_10	20472	21674	STR_RS06895	0.0	Capsule	Immune modulation	99.42	100.00	WP_011227428
KIKOJKAI_10	23418	24566	STR_RS06910	0.0	Capsule	Immune modulation	99.22	100.00	WP_011227431
KIKOJKAI_8	17745	18476	STER_RS05315	0.0	Capsule	Immune modulation	98.50	100.00	WP_011681176
KIKOJKAI_8	17745	18476	STU_RS14645	0.0	Capsule	Immune modulation	98.50	100.00	WP_011226048
KIKOJKAI_8	17745	18476	STR_RS05260	0.0	Capsule	Immune modulation	98.36	100.00	WP_011227248
KIKOJKAI_10	17007	18752	STR_RS06885	0.0	Capsule	Immune modulation	98.22	100.00	WP_011227426
KIKOJKAI_8	18477	19937	STER_RS05320	0.0	Capsule	Immune modulation	97.81	100.00	WP_011681177
KIKOJKAI_8	174624	175385	srtA	0.0	Sortase A	Exoenzyme	97.77	100.00	-
KIKOJKAI_8	18477	19937	STU_RS14650	0.0	Capsule	Immune modulation	97.06	100.21	WP_011226049
KIKOJKAI_8	16294	17034	STU_RS14635	0.0	Capsule	Immune modulation	97.03	100.00	WP_011226046
KIKOJKAI_8	16294	17034	STER_RS05305	0.0	Capsule	Immune modulation	96.90	100.00	WP_011681174
KIKOJKAI_10	29862	30713	rfbD	0.0	Capsule	Immune modulation	96.83	100.00	WP_014621820
KIKOJKAI_8	16299	17034	STR_RS05250	0.0	Capsule	Immune modulation	96.47	98.13	WP_011227246
KIKOJKAI_8	18477	19937	STR_RS05265	0.0	Capsule	Immune modulation	96.23	100.21	WP_041827043
KIKOJKAI_8	14873	16238	STER_RS05300	0.0	Capsule	Immune modulation	95.39	99.85	WP_011681173
KIKOJKAI_8	17044	17736	STR_RS05255	0.0	Capsule	Immune modulation	95.25	99.86	WP_011227247
KIKOJKAI_8	17044	17736	STU_RS14640	0.0	Capsule	Immune modulation	95.25	99.86	WP_011226047
KIKOJKAI_8	14871	16238	STU_RS14630	0.0	Capsule	Immune modulation	94.37	100.00	WP_011226045
KIKOJKAI_8	17044	17736	STER_RS05310	0.0	Capsule	Immune modulation	89.61	100.00	WP_011681175
KIKOJKAI_29	95548	96851	eno	0.0	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
KIKOJKAI_14	123	1133	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
KIKOJKAI_10	21674	22480	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
KIKOJKAI_25	6383	7666	tig/ropA	0.0	Trigger factor	Stress survival	80.73	99.77	WP_000107753
KIKOJKAI_8	137785	138831	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
KIKOJKAI_8	5340	5923	SPD_RS01775	1.60e-147	Capsule	Immune modulation	83.11	96.04	WP_001819836
KIKOJKAI_10	20937	21674	SGO_RS05025	0.0	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
KIKOJKAI_30	27707	28780	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
KIKOJKAI_8	178072	179055	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.21	100.00	AAK75326
KIKOJKAI_29	13309	14277	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
KIKOJKAI_11	57612	59915	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
KIKOJKAI_8	258289	259770	SPD_0310	0.0	UPF0371 protein SPD_0310	92.71	100.00	ABJ54480
KIKOJKAI_30	49626	51872	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
KIKOJKAI_12	11076	12074	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
KIKOJKAI_1	4306	5277	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
KIKOJKAI_10	79640	78489	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
KIKOJKAI_10	43750	41762	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
KIKOJKAI_8	161873	160143	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
KIKOJKAI_19	62257	60779	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
KIKOJKAI_29	95548	96849	Eno	0.0	enolase	86.21	99.77	ABP90468
KIKOJKAI_19	47519	45627	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
KIKOJKAI_30	54797	53088	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
KIKOJKAI_15	22356	23366	pdh	0.0	pyruvate dehydrogenase	82.79	99.70	ABP91006
KIKOJKAI_6	92252	90891	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
KIKOJKAI_15	72674	70782	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
KIKOJKAI_29	81113	82003	bglB	1.23e-179	putative phospho--glucosidase	88.89	99.66	AAK33487
KIKOJKAI_6	11841	10906	SP_0676	4.67e-169	transcription facot	81.73	96.89	AAK74821
KIKOJKAI_30	52064	52921	metF	4.99e-166	methionine biosynthesis enzymes	86.36	99.31	AAK74739
KIKOJKAI_8	56737	56033	covR	6.41e-132	responder protein	85.53	100.00	AAK71319
KIKOJKAI_8	230185	229514	rr01	4.39e-125	response regulator	83.93	99.56	CAB54566
KIKOJKAI_8	56737	56033	walR	2.70e-122	DNA-binding response regulator	82.13	100.00	ANC99504
KIKOJKAI_30	58751	59419	CiaR	1.48e-120	two-component response regulator	84.75	99.55	AAK74935
KIKOJKAI_26	73665	74351	CovR	6.59e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
KIKOJKAI_30	58751	59422	ciaR	2.92e-118	two component system response regulator	82.59	99.12	AIK75708
KIKOJKAI_12	12350	12724	SpxA2	7.97e-72	transcriptional regulator	91.20	94.70	ERL21461
KIKOJKAI_6	103717	104118	SpxA1	2.39e-67	transcriptional regulator	80.60	100.00	ERL19273
KIKOJKAI_26	96049	96336	spr0479	9.31e-49	essential gene	86.46	98.97	AAK99283
KIKOJKAI_27	126308	126562	spr1327	1.58e-44	essential gene	94.12	100.00	AAL00131
KIKOJKAI_19	13871	14134	spr0175	3.45e-42	pathogenitcity related	84.09	100.00	AAK98979
KIKOJKAI_19	14698	14964	spr0177	7.55e-34	essential gene	84.27	88.12	AAK98981
KIKOJKAI_25	7824	8324	RopE (M5005_Spy_1611)	2.27e-76	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
KIKOJKAI_11	57612	59909	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
KIKOJKAI_15	58220	57201	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
KIKOJKAI_22	378	1328	rep37	954 / 948	87.21	AF177168

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
KIKOJKAI_1	0.0	1.0	0.0
KIKOJKAI_2	0.0	1.0	0.0
KIKOJKAI_3	0.0	1.0	0.0
KIKOJKAI_4	1.0	0.0	1.0
KIKOJKAI_5	1.0	0.0	1.0
KIKOJKAI_6	0.0	1.0	0.0
KIKOJKAI_7	0.0	1.0	0.0
KIKOJKAI_8	0.0	1.0	0.0
KIKOJKAI_9	0.0	1.0	0.0
KIKOJKAI_10	0.0	1.0	0.0
KIKOJKAI_11	0.0	1.0	0.0
KIKOJKAI_12	0.0	1.0	0.0
KIKOJKAI_13	0.0	1.0	0.0
KIKOJKAI_14	0.0	1.0	0.0
KIKOJKAI_15	0.0	1.0	0.0
KIKOJKAI_16	1.0	0.0	1.0
KIKOJKAI_17	1.0	0.0	1.0
KIKOJKAI_18	0.0	1.0	0.0
KIKOJKAI_19	0.0	1.0	0.0
KIKOJKAI_20	1.0	0.0	1.0
KIKOJKAI_22	1.0	0.0	1.0
KIKOJKAI_23	0.0	1.0	0.0
KIKOJKAI_24	0.0	1.0	0.0
KIKOJKAI_25	0.0	1.0	0.0
KIKOJKAI_26	0.0	1.0	0.0
KIKOJKAI_27	0.0	1.0	0.0
KIKOJKAI_28	0.0	1.0	0.0
KIKOJKAI_29	0.0	1.0	0.0
KIKOJKAI_30	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found