safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	33	47.38

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
KKGJEFNB_1	20045	20884	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
KKGJEFNB_9	29809	30660	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
KKGJEFNB_7	65326	66087	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
KKGJEFNB_6	34402	35325	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
KKGJEFNB_6	35285	36235	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
KKGJEFNB_6	36260	37342	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
KKGJEFNB_6	37432	38406	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
KKGJEFNB_6	38412	38891	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
KKGJEFNB_6	39084	40202	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
KKGJEFNB_6	41893	42492	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
KKGJEFNB_6	42629	43378	STR_RS05250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227246
KKGJEFNB_6	44089	44820	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
KKGJEFNB_7	29969	30562	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
KKGJEFNB_9	17331	19076	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
KKGJEFNB_9	19078	20778	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
KKGJEFNB_9	21998	22804	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
KKGJEFNB_9	22804	23745	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
KKGJEFNB_9	23742	24890	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
KKGJEFNB_4	74543	76201	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
KKGJEFNB_6	30221	31279	waaB	0.0	Capsule	Immune modulation	99.91	100.00	WP_157822584
KKGJEFNB_6	40770	41729	STR_RS05240	0.0	Capsule	Immune modulation	99.90	100.00	WP_041827042
KKGJEFNB_6	32973	34394	STR_RS05200	0.0	Capsule	Immune modulation	99.86	100.00	WP_011227236
KKGJEFNB_6	43388	44080	STR_RS05255	0.0	Capsule	Immune modulation	99.86	100.00	WP_011227247
KKGJEFNB_7	29969	30562	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
KKGJEFNB_9	20796	21998	STR_RS06895	0.0	Capsule	Immune modulation	99.83	100.00	WP_011227428
KKGJEFNB_7	29969	30562	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
KKGJEFNB_9	19078	20778	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
KKGJEFNB_9	20796	21998	STER_RS07055	0.0	Capsule	Immune modulation	99.58	100.00	WP_011681436
KKGJEFNB_6	43388	44080	STU_RS14640	0.0	Capsule	Immune modulation	99.57	100.00	WP_011226047
KKGJEFNB_9	21998	22804	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
KKGJEFNB_9	22804	23745	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
KKGJEFNB_6	110662	111452	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
KKGJEFNB_9	23742	24890	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
KKGJEFNB_7	30562	31431	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
KKGJEFNB_6	44821	46263	STR_RS05265	0.0	Capsule	Immune modulation	99.11	99.93	WP_041827043
KKGJEFNB_6	44089	44820	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
KKGJEFNB_6	42643	43378	STER_RS05305	0.0	Capsule	Immune modulation	98.50	99.33	WP_011681174
KKGJEFNB_6	44089	44820	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
KKGJEFNB_9	17331	19076	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
KKGJEFNB_6	42643	43378	STU_RS14635	0.0	Capsule	Immune modulation	97.96	99.33	WP_011226046
KKGJEFNB_9	28429	29688	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
KKGJEFNB_6	44821	46263	STU_RS14650	0.0	Capsule	Immune modulation	97.18	99.93	WP_011226049
KKGJEFNB_6	44821	46263	STER_RS05320	0.0	Capsule	Immune modulation	96.17	99.73	WP_011681177
KKGJEFNB_6	43388	44080	STER_RS05310	0.0	Capsule	Immune modulation	89.75	100.00	WP_011681175
KKGJEFNB_25	239015	240318	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
KKGJEFNB_14	197531	198541	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
KKGJEFNB_9	21998	22804	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
KKGJEFNB_21	6320	7603	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
KKGJEFNB_7	28494	29540	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
KKGJEFNB_9	27734	28432	STER_RS07095	5.85e-154	Capsule	Immune modulation	81.15	70.35	WP_011681444
KKGJEFNB_6	40478	40714	STR_RS05235*	4.64e-120	Capsule	Immune modulation	100.00	100.00	-
KKGJEFNB_9	26914	27380	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
KKGJEFNB_9	21261	21998	SGO_RS05025	0.0	Capsule	Immune modulation	85.09	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
KKGJEFNB_7	68774	69757	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
KKGJEFNB_2	40942	42015	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
KKGJEFNB_25	156780	157748	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
KKGJEFNB_10	155193	157496	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
KKGJEFNB_2	61466	63712	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
KKGJEFNB_8	25718	27199	SPD_0310	0.0	UPF0371 protein SPD_0310	91.90	100.00	ABJ54480
KKGJEFNB_1	39676	40668	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
KKGJEFNB_1	3563	4534	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
KKGJEFNB_10	41362	40211	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
KKGJEFNB_10	6889	4907	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
KKGJEFNB_7	52580	50850	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
KKGJEFNB_14	169075	167597	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
KKGJEFNB_25	239015	240316	Eno	0.0	enolase	86.21	99.77	ABP90468
KKGJEFNB_14	154339	152447	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
KKGJEFNB_2	66637	64928	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
KKGJEFNB_14	21202	22212	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
KKGJEFNB_5	7460	6099	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
KKGJEFNB_14	71533	69641	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
KKGJEFNB_25	224585	225475	bglB	4.51e-179	putative phospho--glucosidase	88.55	99.66	AAK33487
KKGJEFNB_4	13510	12575	SP_0676	3.69e-169	transcription facot	81.73	96.89	AAK74821
KKGJEFNB_2	63904	64761	metF	3.37e-166	methionine biosynthesis enzymes	86.36	99.31	AAK74739
KKGJEFNB_6	82963	82259	covR	2.37e-131	responder protein	84.68	100.00	AAK71319
KKGJEFNB_7	121957	121286	rr01	1.97e-126	response regulator	84.38	99.56	CAB54566
KKGJEFNB_6	82963	82259	walR	1.70e-121	DNA-binding response regulator	80.85	100.00	ANC99504
KKGJEFNB_23	66035	66721	CovR	4.11e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
KKGJEFNB_2	70590	71240	CiaR	5.41e-118	two-component response regulator	86.17	96.88	AAK74935
KKGJEFNB_2	70590	71240	ciaR	2.78e-115	two component system response regulator	83.87	96.02	AIK75708
KKGJEFNB_1	40965	41360	SpxA2	8.28e-76	transcriptional regulator	90.91	100.00	ERL21461
KKGJEFNB_5	18818	19219	SpxA1	1.06e-67	transcriptional regulator	80.60	100.00	ERL19273
KKGJEFNB_24	13452	13739	spr0479	3.36e-49	essential gene	86.46	98.97	AAK99283
KKGJEFNB_25	122106	122360	spr1327	4.28e-45	essential gene	95.29	100.00	AAL00131
KKGJEFNB_14	120695	120958	spr0175	1.50e-41	pathogenitcity related	82.95	100.00	AAK98979
KKGJEFNB_14	121522	121788	spr0177	2.62e-33	essential gene	84.27	88.12	AAK98981
KKGJEFNB_21	7761	8261	RopE (M5005_Spy_1611)	4.44e-76	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
KKGJEFNB_10	155193	157490	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
KKGJEFNB_14	57072	56053	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
KKGJEFNB_16	1	487	rep35	487 / 672	100.00	AF295100

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
KKGJEFNB_1	0.0	1.0	0.0
KKGJEFNB_2	0.0	1.0	0.0
KKGJEFNB_3	0.0	1.0	0.0
KKGJEFNB_4	0.0	1.0	0.0
KKGJEFNB_5	0.0	1.0	0.0
KKGJEFNB_6	0.0	1.0	0.0
KKGJEFNB_7	0.0	1.0	0.0
KKGJEFNB_8	0.0	1.0	0.0
KKGJEFNB_9	0.0	1.0	0.0
KKGJEFNB_10	0.0	1.0	0.0
KKGJEFNB_11	0.0	1.0	0.0
KKGJEFNB_12	0.0	1.0	0.0
KKGJEFNB_13	0.0	1.0	0.0
KKGJEFNB_14	0.0	1.0	0.0
KKGJEFNB_16	1.0	0.0	1.0
KKGJEFNB_17	1.0	0.0	1.0
KKGJEFNB_18	0.0	1.0	0.0
KKGJEFNB_19	0.0	1.0	0.0
KKGJEFNB_20	0.0	1.0	0.0
KKGJEFNB_21	0.0	1.0	0.0
KKGJEFNB_22	0.0	1.0	0.0
KKGJEFNB_23	0.0	1.0	0.0
KKGJEFNB_24	0.0	1.0	0.0
KKGJEFNB_25	0.0	1.0	0.0
KKGJEFNB_26	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found