Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 28 | 33 | 43.28 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JPLHIAEP_35 | 36115 | 36924 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| JPLHIAEP_22 | 13812 | 14504 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 99.71 | 100.00 | WP_011226047 |
| JPLHIAEP_20 | 13174 | 14832 | fbp54 | 0.0 | FBPs | Adherence | 99.46 | 100.00 | WP_011227179 |
| JPLHIAEP_35 | 32054 | 33838 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.44 | 100.00 | WP_011226325 |
| JPLHIAEP_35 | 20783 | 21622 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.41 | 100.00 | WP_011226733 |
| JPLHIAEP_35 | 36908 | 37864 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.37 | 100.00 | WP_011226328 |
| JPLHIAEP_35 | 34910 | 36115 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.25 | 100.00 | WP_011226326 |
| JPLHIAEP_35 | 33835 | 34890 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | WP_002948022 |
| JPLHIAEP_22 | 13812 | 14504 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 99.13 | 100.00 | WP_011227247 |
| JPLHIAEP_26 | 3484 | 4353 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| JPLHIAEP_26 | 2891 | 3484 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 98.99 | 100.00 | WP_011226149 |
| JPLHIAEP_26 | 2891 | 3484 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 98.82 | 100.00 | WP_011226149 |
| JPLHIAEP_26 | 2891 | 3484 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 98.82 | 100.00 | WP_011226149 |
| JPLHIAEP_35 | 40705 | 41667 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.65 | 95.25 | WP_011226330 |
| JPLHIAEP_22 | 14513 | 15244 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011681176 |
| JPLHIAEP_35 | 37861 | 39009 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 98.61 | 100.00 | WP_041828290 |
| JPLHIAEP_26 | 38242 | 39003 | srtA | 0.0 | Sortase A | Exoenzyme | 98.56 | 100.00 | - |
| JPLHIAEP_22 | 15245 | 16705 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.43 | 100.00 | WP_011681177 |
| JPLHIAEP_22 | 14513 | 15244 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011226048 |
| JPLHIAEP_22 | 14513 | 15244 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011227248 |
| JPLHIAEP_35 | 41670 | 42941 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.64 | 99.76 | WP_011226331 |
| JPLHIAEP_22 | 15245 | 16705 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.12 | 100.21 | WP_011226049 |
| JPLHIAEP_35 | 44044 | 44895 | rfbD | 0.0 | Capsule | Immune modulation | 96.83 | 100.00 | WP_014621820 |
| JPLHIAEP_22 | 10382 | 11378 | STU_RS14625* | 0.0 | Capsule | Immune modulation | 96.79 | 100.00 | - |
| JPLHIAEP_22 | 15245 | 16705 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.17 | 100.21 | WP_041827043 |
| JPLHIAEP_5 | 170882 | 175777 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 96.02 | 100.00 | WP_000167699 |
| JPLHIAEP_5 | 170824 | 175777 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 95.82 | 100.00 | YP_001200536 |
| JPLHIAEP_5 | 170824 | 175777 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 95.80 | 100.00 | YP_001198331 |
| JPLHIAEP_22 | 11622 | 12987 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.73 | 99.85 | WP_011681173 |
| JPLHIAEP_22 | 11620 | 12987 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.79 | 100.00 | WP_011226045 |
| JPLHIAEP_22 | 13044 | 13802 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 93.54 | 100.94 | WP_011681174 |
| JPLHIAEP_22 | 13044 | 13802 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.15 | 100.94 | WP_011226046 |
| JPLHIAEP_22 | 13049 | 13802 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.84 | 99.07 | WP_011227246 |
| JPLHIAEP_22 | 81269 | 82047 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 92.81 | 99.37 | - |
| JPLHIAEP_22 | 13812 | 14504 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011681175 |
| JPLHIAEP_5 | 95310 | 96613 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.02 | 99.54 | WP_000022832 |
| JPLHIAEP_47 | 570 | 1580 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.86 | 99.90 | WP_002986042 |
| JPLHIAEP_34 | 2769 | 4052 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| JPLHIAEP_26 | 1415 | 2461 | rfbB | 0.0 | Capsule | Immune modulation | 80.42 | 100.00 | WP_009659423 |
| JPLHIAEP_22 | 9924 | 10373 | STU_RS14620 | 1.30e-178 | Capsule | Immune modulation | 92.00 | 100.00 | WP_011226042 |
| JPLHIAEP_22 | 2636 | 3219 | SPD_RS01775 | 2.26e-151 | Capsule | Immune modulation | 83.42 | 96.20 | WP_001819836 |
| JPLHIAEP_22 | 9617 | 9924 | STU_RS14615 | 4.89e-148 | Capsule | Immune modulation | 97.73 | 63.37 | WP_011226041 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JPLHIAEP_5 | 170846 | 175774 | B9H01_RS04765 | 0.0 | glucan-binding protein | 96.59 | 100.00 | AUS90958 |
| JPLHIAEP_11 | 5331 | 6404 | MntE | 0.0 | manganese homeostasis | 94.97 | 99.72 | ABF32167 |
| JPLHIAEP_26 | 41690 | 42673 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| JPLHIAEP_5 | 13300 | 14268 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| JPLHIAEP_38 | 40982 | 43285 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.32 | 99.22 | AAK74619 |
| JPLHIAEP_29 | 18746 | 20227 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| JPLHIAEP_11 | 25854 | 28100 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| JPLHIAEP_35 | 1989 | 991 | recA | 0.0 | DNA recombination and repair | 90.99 | 85.82 | AAL00560 |
| JPLHIAEP_1 | 4669 | 5640 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| JPLHIAEP_35 | 58402 | 56414 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.14 | 100.00 | AE014259_2 |
| JPLHIAEP_35 | 99302 | 98151 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| JPLHIAEP_26 | 25503 | 23773 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| JPLHIAEP_54 | 28128 | 26650 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| JPLHIAEP_5 | 95310 | 96611 | Eno | 0.0 | enolase | 85.98 | 99.77 | ABP90468 |
| JPLHIAEP_54 | 12929 | 11037 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| JPLHIAEP_11 | 31025 | 29316 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| JPLHIAEP_49 | 60047 | 61057 | pdh | 0.0 | pyruvate dehydrogenase | 82.20 | 99.70 | ABP91006 |
| JPLHIAEP_21 | 5989 | 4628 | mnmE | 0.0 | central tRNA-modifying GTPase | 81.80 | 99.56 | ABP90420 |
| JPLHIAEP_49 | 110345 | 108453 | PepO | 0.0 | endopeptidase | 80.35 | 100.00 | ABF32980 |
| JPLHIAEP_5 | 80881 | 81771 | bglB | 1.42e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| JPLHIAEP_19 | 12156 | 11218 | SP_0676 | 1.59e-168 | transcription facot | 81.15 | 97.20 | AAK74821 |
| JPLHIAEP_11 | 28292 | 29149 | metF | 3.60e-167 | methionine biosynthesis enzymes | 86.71 | 99.31 | AAK74739 |
| JPLHIAEP_22 | 53563 | 52859 | covR | 1.95e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| JPLHIAEP_28 | 31392 | 30721 | rr01 | 7.12e-127 | response regulator | 84.38 | 99.56 | CAB54566 |
| JPLHIAEP_11 | 34979 | 35647 | CiaR | 1.17e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| JPLHIAEP_11 | 34979 | 35650 | ciaR | 1.26e-123 | two component system response regulator | 85.27 | 99.12 | AIK75708 |
| JPLHIAEP_22 | 53563 | 52859 | walR | 8.20e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| JPLHIAEP_45 | 35851 | 36537 | CovR | 2.35e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| JPLHIAEP_35 | 715 | 320 | SpxA2 | 2.41e-75 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| JPLHIAEP_56 | 13102 | 13389 | spr0479 | 1.18e-48 | essential gene | 84.38 | 98.97 | AAK99283 |
| JPLHIAEP_2 | 10818 | 11072 | spr1327 | 7.68e-46 | essential gene | 95.29 | 100.00 | AAL00131 |
| JPLHIAEP_51 | 7502 | 7765 | spr0175 | 6.76e-43 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| JPLHIAEP_51 | 8329 | 8595 | spr0177 | 2.72e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| JPLHIAEP_34 | 4210 | 4710 | RopE (M5005_Spy_1611) | 2.35e-76 | probable DNA-directed RNA polymerase subunit delta | 82.04 | 87.43 | AAK34603 |
| JPLHIAEP_38 | 41006 | 43279 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.03 | 97.06 | MYN69560 |
| JPLHIAEP_49 | 95907 | 94888 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JPLHIAEP_1 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_2 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_3 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_4 | 1.0 | 0.0 | 1.0 |
| JPLHIAEP_5 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_6 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_7 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_8 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_9 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_10 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_11 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_12 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_13 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_14 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_15 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_16 | 1.0 | 0.0 | 1.0 |
| JPLHIAEP_17 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_18 | 1.0 | 0.0 | 1.0 |
| JPLHIAEP_19 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_20 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_21 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_22 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_23 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_24 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_25 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_26 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_27 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_28 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_29 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_30 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_31 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_32 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_33 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_34 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_35 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_36 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_37 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_38 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_39 | 1.0 | 0.0 | 1.0 |
| JPLHIAEP_40 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_41 | 1.0 | 0.0 | 1.0 |
| JPLHIAEP_42 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_43 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_44 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_45 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_46 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_47 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_48 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_49 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_50 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_51 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_52 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_53 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_54 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_55 | 1.0 | 0.0 | 1.0 |
| JPLHIAEP_56 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_58 | 1.0 | 0.0 | 1.0 |
| JPLHIAEP_59 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_60 | 1.0 | 0.0 | 1.0 |
| JPLHIAEP_61 | 1.0 | 0.0 | 1.0 |
| JPLHIAEP_62 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_63 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_64 | 1.0 | 0.0 | 1.0 |
| JPLHIAEP_65 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_66 | 0.0 | 1.0 | 0.0 |
| JPLHIAEP_67 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JPLHIAEP_5 | 96674 | 136547 | False | Siphoviridae | VOG0275, VOG7656, VOG4602, VOG0743, VOG3622, VOG1564, VOG9469, VOG0651, VOG4566, VOG0189, VOG3430, VOG1309, VOG0685, VOG0226, VOG4693, VOG0655, VOG0656, VOG0152, VOG4571, VOG0152, VOG6495, VOG0657, VOG5439, VOG0658, VOG0198, VOG0796, VOG4544, VOG0720, VOG4564, VOG3434, VOG1887, VOG3435, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG4545, VOG4605, VOG4599, VOG0647, VOG4782, VOG3298, VOG0641, VOG0054, VOG4649, VOG3299, VOG3300 |
Phigaro v2.4.0 (Database: Jan. 2024 release)