Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 29 | 33 | 43.93 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LFBKNOKG_21 | 9354 | 9947 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| LFBKNOKG_21 | 9947 | 10816 | rfbA | 0.0 | Capsule | Immune modulation | 99.89 | 100.00 | WP_011681286 |
| LFBKNOKG_30 | 18700 | 19509 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| LFBKNOKG_19 | 9983 | 10675 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 99.86 | 100.00 | WP_011226047 |
| LFBKNOKG_14 | 16135 | 16758 | STU_RS14555 | 0.0 | Capsule | Immune modulation | 99.84 | 100.00 | WP_011226030 |
| LFBKNOKG_21 | 9354 | 9947 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| LFBKNOKG_30 | 20446 | 21594 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| LFBKNOKG_21 | 9354 | 9947 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| LFBKNOKG_17 | 644 | 2302 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| LFBKNOKG_30 | 14639 | 16423 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.55 | 100.00 | WP_011226325 |
| LFBKNOKG_1 | 21210 | 22049 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| LFBKNOKG_14 | 16869 | 17987 | STU_RS14550 | 0.0 | Capsule | Immune modulation | 99.46 | 100.00 | WP_011226029 |
| LFBKNOKG_30 | 19493 | 20449 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.37 | 100.00 | WP_011226328 |
| LFBKNOKG_19 | 9983 | 10675 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011227247 |
| LFBKNOKG_19 | 10684 | 11415 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 99.18 | 100.00 | WP_011226048 |
| LFBKNOKG_30 | 16420 | 17475 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | WP_002948022 |
| LFBKNOKG_30 | 17495 | 18700 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.09 | 100.00 | WP_011226326 |
| LFBKNOKG_19 | 10684 | 11415 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | WP_011227248 |
| LFBKNOKG_19 | 10684 | 11415 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| LFBKNOKG_18 | 40455 | 41870 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.80 | 100.00 | WP_011681165 |
| LFBKNOKG_30 | 24253 | 25527 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | WP_011226331 |
| LFBKNOKG_30 | 23291 | 24253 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.65 | 95.25 | WP_011226330 |
| LFBKNOKG_20 | 830 | 1623 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.24 | 100.25 | - |
| LFBKNOKG_19 | 11416 | 12876 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.15 | 100.00 | WP_011681177 |
| LFBKNOKG_22 | 14658 | 15419 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| LFBKNOKG_19 | 11416 | 12876 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.40 | 100.21 | WP_011226049 |
| LFBKNOKG_14 | 14771 | 15220 | STU_RS14620 | 0.0 | Capsule | Immune modulation | 97.33 | 100.00 | WP_011226042 |
| LFBKNOKG_19 | 9233 | 9973 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.17 | 100.00 | WP_011681174 |
| LFBKNOKG_19 | 9233 | 9973 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.03 | 100.00 | WP_011226046 |
| LFBKNOKG_19 | 9238 | 9973 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.47 | 98.13 | WP_011227246 |
| LFBKNOKG_19 | 11416 | 12876 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.30 | 100.21 | WP_041827043 |
| LFBKNOKG_30 | 26628 | 27479 | rfbD | 0.0 | Capsule | Immune modulation | 96.24 | 100.00 | WP_014621820 |
| LFBKNOKG_19 | 7809 | 9176 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.74 | 99.93 | WP_011226045 |
| LFBKNOKG_19 | 7811 | 9176 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.37 | 99.71 | WP_011681173 |
| LFBKNOKG_14 | 13767 | 14762 | STU_RS14625* | 0.0 | Capsule | Immune modulation | 92.08 | 99.80 | - |
| LFBKNOKG_19 | 9983 | 10675 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.75 | 100.00 | WP_011681175 |
| LFBKNOKG_6 | 35949 | 37252 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| LFBKNOKG_35 | 1212 | 2222 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.46 | 99.80 | WP_002986042 |
| LFBKNOKG_47 | 6303 | 7586 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| LFBKNOKG_21 | 7879 | 8925 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| LFBKNOKG_14 | 15220 | 15529 | STU_RS14615 | 6.82e-111 | Capsule | Immune modulation | 90.32 | 63.58 | WP_011226041 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LFBKNOKG_22 | 18106 | 19089 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| LFBKNOKG_7 | 59735 | 60808 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| LFBKNOKG_33 | 15948 | 18251 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| LFBKNOKG_25 | 34497 | 35978 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.71 | 100.00 | ABJ54480 |
| LFBKNOKG_5 | 13301 | 14269 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| LFBKNOKG_8 | 6356 | 8602 | metE | 0.0 | methionine biosynthesis enzymes | 91.86 | 100.00 | AAK74738 |
| LFBKNOKG_1 | 40844 | 41842 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| LFBKNOKG_1 | 4726 | 5697 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| LFBKNOKG_31 | 19222 | 18071 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| LFBKNOKG_30 | 40657 | 38669 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.14 | 100.00 | AE014259_2 |
| LFBKNOKG_22 | 1901 | 171 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| LFBKNOKG_40 | 55648 | 54170 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| LFBKNOKG_6 | 35949 | 37250 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| LFBKNOKG_40 | 40459 | 38567 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| LFBKNOKG_8 | 11527 | 9818 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| LFBKNOKG_36 | 21573 | 22583 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| LFBKNOKG_17 | 27543 | 26182 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| LFBKNOKG_37 | 49018 | 47126 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| LFBKNOKG_6 | 21514 | 22404 | bglB | 2.19e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| LFBKNOKG_15 | 4180 | 3242 | SP_0676 | 4.84e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| LFBKNOKG_8 | 8794 | 9651 | metF | 1.42e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| LFBKNOKG_19 | 49663 | 48959 | covR | 9.01e-133 | responder protein | 85.53 | 100.00 | AAK71319 |
| LFBKNOKG_25 | 9832 | 9161 | rr01 | 6.23e-126 | response regulator | 83.93 | 99.56 | CAB54566 |
| LFBKNOKG_19 | 49663 | 48959 | walR | 8.47e-123 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| LFBKNOKG_8 | 15481 | 16149 | CiaR | 1.89e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| LFBKNOKG_49 | 73943 | 74629 | CovR | 6.60e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| LFBKNOKG_8 | 15481 | 16152 | ciaR | 1.86e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| LFBKNOKG_1 | 42118 | 42492 | SpxA2 | 2.66e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| LFBKNOKG_17 | 38903 | 39304 | SpxA1 | 1.00e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| LFBKNOKG_49 | 96326 | 96613 | spr0479 | 9.32e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| LFBKNOKG_2 | 124662 | 124916 | spr1327 | 1.55e-44 | essential gene | 94.12 | 100.00 | AAL00131 |
| LFBKNOKG_40 | 6814 | 7077 | spr0175 | 3.24e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| LFBKNOKG_40 | 7641 | 7907 | spr0177 | 7.09e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| LFBKNOKG_47 | 7744 | 8244 | RopE (M5005_Spy_1611) | 1.63e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| LFBKNOKG_33 | 15948 | 18245 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| LFBKNOKG_37 | 34547 | 33528 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LFBKNOKG_1 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_2 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_3 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_4 | 1.0 | 0.0 | 1.0 |
| LFBKNOKG_5 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_6 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_7 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_8 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_9 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_10 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_11 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_12 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_13 | 1.0 | 0.0 | 1.0 |
| LFBKNOKG_14 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_15 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_16 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_17 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_18 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_19 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_20 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_21 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_22 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_23 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_24 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_25 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_26 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_27 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_28 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_29 | 1.0 | 0.0 | 1.0 |
| LFBKNOKG_30 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_31 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_32 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_33 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_34 | 1.0 | 0.0 | 1.0 |
| LFBKNOKG_35 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_36 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_37 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_38 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_39 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_40 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_42 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_43 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_44 | 1.0 | 0.0 | 1.0 |
| LFBKNOKG_45 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_46 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_47 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_48 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_49 | 0.0 | 1.0 | 0.0 |
| LFBKNOKG_50 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)