Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 33 | 39.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NHAMOFJJ_21 | 115859 | 116728 | rfbA | 0.0 | Capsule | Immune modulation | 99.89 | 100.00 | WP_011681286 |
| NHAMOFJJ_21 | 115266 | 115859 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| NHAMOFJJ_13 | 207841 | 208650 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_002948020 |
| NHAMOFJJ_21 | 115266 | 115859 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| NHAMOFJJ_9 | 13047 | 14705 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| NHAMOFJJ_1 | 20071 | 20910 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| NHAMOFJJ_21 | 115266 | 115859 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| NHAMOFJJ_13 | 209587 | 210735 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | WP_041828290 |
| NHAMOFJJ_13 | 208634 | 209590 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226328 |
| NHAMOFJJ_13 | 203782 | 205564 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.10 | 99.89 | WP_011226325 |
| NHAMOFJJ_10 | 13217 | 13957 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | WP_011226046 |
| NHAMOFJJ_13 | 206636 | 207841 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | WP_011226326 |
| NHAMOFJJ_10 | 13217 | 13957 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | WP_011681174 |
| NHAMOFJJ_10 | 81394 | 82186 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.49 | 100.13 | - |
| NHAMOFJJ_13 | 205561 | 206616 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 98.48 | 100.00 | WP_002948022 |
| NHAMOFJJ_13 | 212159 | 213010 | rfbD | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_014621820 |
| NHAMOFJJ_10 | 13222 | 13957 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.23 | 98.13 | WP_011227246 |
| NHAMOFJJ_10 | 15400 | 16860 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.74 | 100.00 | WP_011681177 |
| NHAMOFJJ_10 | 14668 | 15399 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011681176 |
| NHAMOFJJ_10 | 14668 | 15399 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011226048 |
| NHAMOFJJ_13 | 14651 | 15412 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| NHAMOFJJ_10 | 14668 | 15399 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011227248 |
| NHAMOFJJ_10 | 13967 | 14659 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 97.26 | 100.00 | WP_011681175 |
| NHAMOFJJ_10 | 15400 | 16860 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.58 | 100.21 | WP_011226049 |
| NHAMOFJJ_10 | 15400 | 16860 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.76 | 100.21 | WP_041827043 |
| NHAMOFJJ_10 | 11795 | 13160 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.09 | 99.85 | WP_011681173 |
| NHAMOFJJ_10 | 11793 | 13160 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.01 | 100.00 | WP_011226045 |
| NHAMOFJJ_10 | 13967 | 14659 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 90.62 | 100.00 | WP_011226047 |
| NHAMOFJJ_10 | 13967 | 14659 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 90.04 | 100.00 | WP_011227247 |
| NHAMOFJJ_26 | 94478 | 95781 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| NHAMOFJJ_14 | 184 | 1194 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.46 | 99.80 | WP_002986042 |
| NHAMOFJJ_20 | 5541 | 6824 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| NHAMOFJJ_21 | 113791 | 114837 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NHAMOFJJ_13 | 18099 | 19082 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.51 | 100.00 | AAK75326 |
| NHAMOFJJ_26 | 197106 | 198179 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| NHAMOFJJ_13 | 386778 | 389081 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| NHAMOFJJ_26 | 12168 | 13136 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| NHAMOFJJ_13 | 108620 | 110101 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| NHAMOFJJ_2 | 18165 | 20411 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| NHAMOFJJ_1 | 49268 | 50266 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| NHAMOFJJ_1 | 3587 | 4558 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| NHAMOFJJ_13 | 256029 | 254878 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| NHAMOFJJ_13 | 227636 | 225648 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| NHAMOFJJ_13 | 1902 | 172 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| NHAMOFJJ_17 | 62691 | 61213 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| NHAMOFJJ_26 | 94478 | 95779 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| NHAMOFJJ_17 | 47502 | 45610 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| NHAMOFJJ_2 | 23335 | 21626 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| NHAMOFJJ_15 | 21571 | 22581 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| NHAMOFJJ_9 | 35870 | 34509 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| NHAMOFJJ_16 | 48565 | 46673 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| NHAMOFJJ_26 | 80041 | 80931 | bglB | 1.44e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| NHAMOFJJ_7 | 11845 | 10907 | SP_0676 | 8.45e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| NHAMOFJJ_2 | 20603 | 21460 | metF | 1.38e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| NHAMOFJJ_10 | 53707 | 53003 | covR | 1.96e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| NHAMOFJJ_13 | 70749 | 70078 | rr01 | 8.21e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| NHAMOFJJ_10 | 53707 | 53003 | walR | 8.28e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| NHAMOFJJ_2 | 27289 | 27957 | CiaR | 3.23e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| NHAMOFJJ_21 | 97599 | 98285 | CovR | 7.38e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| NHAMOFJJ_2 | 27289 | 27960 | ciaR | 3.18e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| NHAMOFJJ_1 | 50542 | 50916 | SpxA2 | 3.18e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| NHAMOFJJ_9 | 47233 | 47634 | SpxA1 | 2.13e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| NHAMOFJJ_22 | 13690 | 13977 | spr0479 | 3.00e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| NHAMOFJJ_24 | 129214 | 129468 | spr1327 | 2.40e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| NHAMOFJJ_17 | 13874 | 14137 | spr0175 | 3.50e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| NHAMOFJJ_17 | 14701 | 14967 | spr0177 | 7.66e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| NHAMOFJJ_20 | 6982 | 7482 | RopE (M5005_Spy_1611) | 1.61e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| NHAMOFJJ_13 | 386778 | 389075 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| NHAMOFJJ_16 | 34105 | 33086 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NHAMOFJJ_1 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_2 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_3 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_4 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_5 | 1.0 | 0.0 | 1.0 |
| NHAMOFJJ_6 | 1.0 | 0.0 | 1.0 |
| NHAMOFJJ_7 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_8 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_9 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_10 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_11 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_12 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_13 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_14 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_15 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_16 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_17 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_19 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_20 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_21 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_22 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_23 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_24 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_25 | 0.0 | 1.0 | 0.0 |
| NHAMOFJJ_26 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)