Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 33 | 40.22 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EMADLBLA_9 | 53598 | 54467 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| EMADLBLA_9 | 53005 | 53598 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| EMADLBLA_11 | 9420 | 10229 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| EMADLBLA_9 | 53005 | 53598 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| EMADLBLA_9 | 53005 | 53598 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| EMADLBLA_6 | 13094 | 14752 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| EMADLBLA_11 | 7140 | 8195 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.62 | 100.00 | WP_002948022 |
| EMADLBLA_12 | 21208 | 22047 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| EMADLBLA_8 | 6012 | 6743 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | WP_011681176 |
| EMADLBLA_11 | 8215 | 9420 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.25 | 100.00 | WP_011226326 |
| EMADLBLA_11 | 11166 | 12314 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.22 | 100.00 | WP_041828290 |
| EMADLBLA_11 | 10213 | 11169 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226328 |
| EMADLBLA_11 | 5361 | 7143 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.10 | 99.89 | WP_011226325 |
| EMADLBLA_8 | 6012 | 6743 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011226048 |
| EMADLBLA_8 | 6012 | 6743 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011227248 |
| EMADLBLA_8 | 72880 | 73673 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.36 | 100.25 | - |
| EMADLBLA_8 | 6744 | 8204 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.29 | 100.00 | WP_011681177 |
| EMADLBLA_9 | 88369 | 89130 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| EMADLBLA_8 | 5311 | 6003 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.55 | 100.00 | WP_011226047 |
| EMADLBLA_11 | 13738 | 14589 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| EMADLBLA_8 | 5311 | 6003 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.26 | 100.00 | WP_011227247 |
| EMADLBLA_8 | 4561 | 5301 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.03 | 100.00 | WP_011681174 |
| EMADLBLA_8 | 4561 | 5301 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.90 | 100.00 | WP_011226046 |
| EMADLBLA_8 | 6744 | 8204 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.85 | 100.21 | WP_011226049 |
| EMADLBLA_8 | 4566 | 5301 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.47 | 98.13 | WP_011227246 |
| EMADLBLA_8 | 6744 | 8204 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.89 | 100.21 | WP_041827043 |
| EMADLBLA_8 | 3137 | 4504 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.11 | 99.93 | WP_011226045 |
| EMADLBLA_8 | 3139 | 4504 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.66 | 99.78 | WP_011681173 |
| EMADLBLA_8 | 5311 | 6003 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.62 | 100.00 | WP_011681175 |
| EMADLBLA_25 | 229201 | 230504 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| EMADLBLA_13 | 123 | 1133 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.46 | 99.80 | WP_002986042 |
| EMADLBLA_23 | 9808 | 11091 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| EMADLBLA_9 | 51530 | 52576 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| EMADLBLA_7 | 1313 | 1896 | SPD_RS01775 | 5.10e-149 | Capsule | Immune modulation | 83.08 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EMADLBLA_9 | 91817 | 92800 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| EMADLBLA_26 | 27718 | 28791 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| EMADLBLA_11 | 185461 | 187764 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| EMADLBLA_25 | 146971 | 147939 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| EMADLBLA_9 | 162896 | 164377 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| EMADLBLA_26 | 48244 | 50490 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| EMADLBLA_12 | 40839 | 41837 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| EMADLBLA_12 | 4726 | 5697 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| EMADLBLA_11 | 64240 | 63089 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| EMADLBLA_11 | 27761 | 25773 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| EMADLBLA_9 | 75618 | 73888 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.39 | 100.00 | AAK34199 |
| EMADLBLA_18 | 27051 | 25573 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| EMADLBLA_25 | 229201 | 230502 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| EMADLBLA_18 | 12314 | 10422 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| EMADLBLA_26 | 53415 | 51706 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| EMADLBLA_15 | 19144 | 20154 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| EMADLBLA_6 | 40039 | 38678 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| EMADLBLA_16 | 48989 | 47097 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| EMADLBLA_25 | 214765 | 215655 | bglB | 2.27e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| EMADLBLA_5 | 11946 | 11008 | SP_0676 | 7.02e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| EMADLBLA_26 | 50682 | 51539 | metF | 8.15e-166 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| EMADLBLA_8 | 45045 | 44341 | covR | 1.76e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| EMADLBLA_9 | 143916 | 143245 | rr01 | 3.91e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| EMADLBLA_26 | 57370 | 58038 | CiaR | 2.79e-124 | two-component response regulator | 86.10 | 99.55 | AAK74935 |
| EMADLBLA_8 | 45045 | 44341 | walR | 7.43e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| EMADLBLA_26 | 57370 | 58041 | ciaR | 3.78e-122 | two component system response regulator | 83.93 | 99.12 | AIK75708 |
| EMADLBLA_23 | 164231 | 164917 | CovR | 3.54e-119 | serves to repress GAS virulence factor-encoding genes | 81.22 | 100.00 | ACE75886 |
| EMADLBLA_12 | 42113 | 42487 | SpxA2 | 2.66e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| EMADLBLA_6 | 51457 | 51858 | SpxA1 | 2.14e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| EMADLBLA_23 | 186623 | 186910 | spr0479 | 1.63e-48 | essential gene | 86.46 | 98.97 | AAK99283 |
| EMADLBLA_25 | 106771 | 107025 | spr1327 | 4.71e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| EMADLBLA_17 | 15839 | 16102 | spr0175 | 1.46e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| EMADLBLA_17 | 16666 | 16932 | spr0177 | 3.19e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| EMADLBLA_23 | 11249 | 11749 | RopE (M5005_Spy_1611) | 5.64e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| EMADLBLA_11 | 185461 | 187758 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| EMADLBLA_16 | 34530 | 33511 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EMADLBLA_1 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_2 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_3 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_4 | 1.0 | 0.0 | 1.0 |
| EMADLBLA_5 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_6 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_7 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_8 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_9 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_10 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_11 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_12 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_13 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_14 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_15 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_16 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_17 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_18 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_19 | 1.0 | 0.0 | 1.0 |
| EMADLBLA_20 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_21 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_22 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_23 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_24 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_25 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_26 | 0.0 | 1.0 | 0.0 |
| EMADLBLA_27 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)