safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	35	33	48.10

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
IEHOACBJ_15	54813	55682	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
IEHOACBJ_14	1026	2441	STER_RS05250	Capsule	Immune modulation	100.00	100.00	WP_011681165
IEHOACBJ_14	3642	4622	STER_RS05260	Capsule	Immune modulation	100.00	100.00	WP_011681166
IEHOACBJ_14	4615	5799	STER_RS05265	Capsule	Immune modulation	100.00	100.00	WP_011681167
IEHOACBJ_14	5816	6868	STER_RS05270	Capsule	Immune modulation	100.00	100.00	WP_011681168
IEHOACBJ_14	6883	7791	STER_RS05275	Capsule	Immune modulation	100.00	100.00	WP_011681169
IEHOACBJ_14	7812	8873	STER_RS05280	Capsule	Immune modulation	100.00	100.00	WP_202964935
IEHOACBJ_14	8910	9401	STER_RS05285	Capsule	Immune modulation	100.00	100.00	WP_011681171
IEHOACBJ_14	9413	9889	STER_RS05290	Capsule	Immune modulation	100.00	100.00	WP_011681172
IEHOACBJ_14	12069	12809	STER_RS05305	Capsule	Immune modulation	100.00	100.00	WP_011681174
IEHOACBJ_14	12819	13511	STER_RS05310	Capsule	Immune modulation	100.00	100.00	WP_011681175
IEHOACBJ_14	13520	14251	STER_RS05315	Capsule	Immune modulation	100.00	100.00	WP_011681176
IEHOACBJ_14	14252	15712	STER_RS05320	Capsule	Immune modulation	100.00	100.00	WP_011681177
IEHOACBJ_15	54220	54813	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
IEHOACBJ_16	84438	86183	STER_RS07045	Capsule	Immune modulation	100.00	100.00	WP_011681434
IEHOACBJ_16	86185	87885	STER_RS07050	Capsule	Immune modulation	100.00	100.00	WP_041827753
IEHOACBJ_16	87903	89105	STER_RS07055	Capsule	Immune modulation	100.00	100.00	WP_011681436
IEHOACBJ_16	89105	89911	STER_RS07060	Capsule	Immune modulation	100.00	100.00	WP_011681437
IEHOACBJ_16	89911	90852	STER_RS07065	Capsule	Immune modulation	100.00	100.00	WP_011681438
IEHOACBJ_16	90849	91997	STER_RS07070	Capsule	Immune modulation	100.00	100.00	WP_011681439
IEHOACBJ_16	92116	92892	STER_RS07075	Capsule	Immune modulation	100.00	100.00	WP_011681440
IEHOACBJ_16	93238	93954	STER_RS07085	Capsule	Immune modulation	100.00	100.00	WP_011681442
IEHOACBJ_16	93955	94923	STER_RS07090	Capsule	Immune modulation	100.00	100.00	WP_011681443
IEHOACBJ_16	94941	95918	STER_RS07095	Capsule	Immune modulation	100.00	100.00	WP_011681444
IEHOACBJ_16	95915	97174	STER_RS07100	Capsule	Immune modulation	100.00	100.00	WP_011681445
IEHOACBJ_14	10645	12012	STER_RS05300	Capsule	Immune modulation	99.85	100.00	WP_011681173
IEHOACBJ_15	54220	54813	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
IEHOACBJ_16	86185	87885	STR_RS06890	Capsule	Immune modulation	99.71	100.00	WP_041827077
IEHOACBJ_15	54220	54813	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
IEHOACBJ_13	71540	73198	fbp54	FBPs	Adherence	99.64	100.00	WP_011227179
IEHOACBJ_12	19769	20608	cbpD	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
IEHOACBJ_16	89105	89911	STR_RS06900	Capsule	Immune modulation	99.50	100.00	WP_011227429
IEHOACBJ_16	89911	90852	STR_RS06905	Capsule	Immune modulation	99.47	100.00	WP_011227430
IEHOACBJ_16	87903	89105	STR_RS06895	Capsule	Immune modulation	99.42	100.00	WP_011227428
IEHOACBJ_16	90849	91997	STR_RS06910	Capsule	Immune modulation	99.22	100.00	WP_011227431
IEHOACBJ_14	88844	89634	cshA	Cell surface hydrophobicity proteins	Adherence	98.74	100.00	-
IEHOACBJ_14	12074	12809	STR_RS05250	Capsule	Immune modulation	98.50	98.13	WP_011227246
IEHOACBJ_14	12069	12809	STU_RS14635	Capsule	Immune modulation	98.25	100.00	WP_011226046
IEHOACBJ_14	13520	14251	STR_RS05260	Capsule	Immune modulation	98.22	100.00	WP_011227248
IEHOACBJ_16	84438	86183	STR_RS06885	Capsule	Immune modulation	98.22	100.00	WP_011227426
IEHOACBJ_14	13520	14251	STU_RS14645	Capsule	Immune modulation	98.09	100.00	WP_011226048
IEHOACBJ_15	89583	90344	srtA	Sortase A	Exoenzyme	97.64	100.00	-
IEHOACBJ_14	14252	15712	STU_RS14650	Capsule	Immune modulation	97.47	100.21	WP_011226049
IEHOACBJ_14	14252	15712	STR_RS05265	Capsule	Immune modulation	96.92	100.21	WP_041827043
IEHOACBJ_16	97293	98144	rfbD	Capsule	Immune modulation	96.83	100.00	WP_014621820
IEHOACBJ_14	10649	12012	STU_RS14630	Capsule	Immune modulation	94.94	99.71	WP_011226045
IEHOACBJ_14	12819	13511	STU_RS14640	Capsule	Immune modulation	89.90	100.00	WP_011226047
IEHOACBJ_14	12819	13511	STR_RS05255	Capsule	Immune modulation	89.61	100.00	WP_011227247
IEHOACBJ_7	46532	47835	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
IEHOACBJ_19	64485	65495	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
IEHOACBJ_16	89105	89911	SGO_RS05020	Capsule	Immune modulation	82.90	100.00	WP_012000440
IEHOACBJ_31	5482	6765	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
IEHOACBJ_15	52745	53791	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423
IEHOACBJ_16	88368	89105	SGO_RS05025	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
IEHOACBJ_15	93031	94014	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
IEHOACBJ_8	27708	28781	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
IEHOACBJ_18	57600	59903	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
IEHOACBJ_6	17699	18667	spr0084	0.0	pathogenitcity related	92.88	98.48	AAK98888
IEHOACBJ_15	169787	171268	SPD_0310	0.0	UPF0371 protein SPD_0310	92.51	100.00	ABJ54480
IEHOACBJ_8	59618	61864	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
IEHOACBJ_12	39400	40398	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
IEHOACBJ_12	3285	4256	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
IEHOACBJ_16	146348	145197	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
IEHOACBJ_16	111183	109195	uvrB	0.0	excinuclease ABC, B subunit	89.14	100.00	AE014259_2
IEHOACBJ_15	76831	75101	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
IEHOACBJ_22	47331	45853	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
IEHOACBJ_7	46532	47833	Eno	0.0	enolase	86.21	99.77	ABP90468
IEHOACBJ_22	32596	30704	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
IEHOACBJ_8	64789	63080	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
IEHOACBJ_20	23095	24105	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
IEHOACBJ_13	97633	96272	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
IEHOACBJ_21	10876	8984	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
IEHOACBJ_7	32096	32986	bglB	3.48e-179	putative phospho--glucosidase	88.55	99.66	AAK33487
IEHOACBJ_13	17994	17056	SP_0676	7.20e-169	transcription facot	81.47	97.20	AAK74821
IEHOACBJ_8	62056	62913	metF	5.83e-166	methionine biosynthesis enzymes	86.36	99.31	AAK74739
IEHOACBJ_14	52535	51831	covR	2.15e-132	responder protein	85.53	100.00	AAK71319
IEHOACBJ_15	145180	144509	rr01	2.96e-125	response regulator	83.93	99.56	CAB54566
IEHOACBJ_14	52535	51831	walR	9.04e-123	DNA-binding response regulator	82.13	100.00	ANC99504
IEHOACBJ_8	68744	69412	CiaR	7.72e-119	two-component response regulator	84.31	99.55	AAK74935
IEHOACBJ_31	158441	159127	CovR	9.01e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
IEHOACBJ_8	68744	69415	ciaR	7.60e-117	two component system response regulator	82.14	99.12	AIK75708
IEHOACBJ_12	40674	41048	SpxA2	2.56e-71	transcriptional regulator	91.20	94.70	ERL21461
IEHOACBJ_13	108996	109397	SpxA1	3.31e-67	transcriptional regulator	80.60	100.00	ERL19273
IEHOACBJ_32	16726	17013	spr0479	3.23e-49	essential gene	86.46	98.97	AAK99283
IEHOACBJ_3	33747	34001	spr1327	4.18e-45	essential gene	94.12	100.00	AAL00131
IEHOACBJ_21	64718	64981	spr0175	2.54e-42	pathogenitcity related	84.09	100.00	AAK98979
IEHOACBJ_21	65545	65811	spr0177	5.56e-34	essential gene	84.27	88.12	AAK98981
IEHOACBJ_31	6923	7423	RopE (M5005_Spy_1611)	4.41e-76	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
IEHOACBJ_18	57600	59897	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
IEHOACBJ_20	58956	57937	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
IEHOACBJ_26	2155	3099	rep37	945 / 945	100.00	HM050421

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IEHOACBJ_1	0.0	1.0	0.0
IEHOACBJ_2	0.0	1.0	0.0
IEHOACBJ_3	0.0	1.0	0.0
IEHOACBJ_4	0.0	1.0	0.0
IEHOACBJ_5	0.0	1.0	0.0
IEHOACBJ_6	0.0	1.0	0.0
IEHOACBJ_7	0.0	1.0	0.0
IEHOACBJ_8	0.0	1.0	0.0
IEHOACBJ_9	0.0	1.0	0.0
IEHOACBJ_10	0.0	1.0	0.0
IEHOACBJ_11	0.0	1.0	0.0
IEHOACBJ_12	0.0	1.0	0.0
IEHOACBJ_13	0.0	1.0	0.0
IEHOACBJ_14	0.0	1.0	0.0
IEHOACBJ_15	0.0	1.0	0.0
IEHOACBJ_16	0.0	1.0	0.0
IEHOACBJ_17	0.0	1.0	0.0
IEHOACBJ_18	0.0	1.0	0.0
IEHOACBJ_19	0.0	1.0	0.0
IEHOACBJ_20	0.0	1.0	0.0
IEHOACBJ_21	0.0	1.0	0.0
IEHOACBJ_22	0.0	1.0	0.0
IEHOACBJ_23	0.0	1.0	0.0
IEHOACBJ_25	1.0	0.0	1.0
IEHOACBJ_26	1.0	0.0	1.0
IEHOACBJ_27	0.0	1.0	0.0
IEHOACBJ_28	0.0	1.0	0.0
IEHOACBJ_29	0.0	1.0	0.0
IEHOACBJ_30	0.0	1.0	0.0
IEHOACBJ_31	0.0	1.0	0.0
IEHOACBJ_32	0.0	1.0	0.0
IEHOACBJ_33	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found