Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 24 | 33 | 40.80 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OLKLGFOO_14 | 9591 | 10460 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| OLKLGFOO_14 | 8998 | 9591 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| OLKLGFOO_15 | 81410 | 82219 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| OLKLGFOO_14 | 8998 | 9591 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| OLKLGFOO_15 | 82203 | 83159 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| OLKLGFOO_15 | 83156 | 84304 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| OLKLGFOO_14 | 8998 | 9591 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| OLKLGFOO_1 | 20868 | 21707 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| OLKLGFOO_15 | 80205 | 81410 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226326 |
| OLKLGFOO_15 | 79130 | 80185 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.43 | 100.00 | WP_002948022 |
| OLKLGFOO_10 | 13045 | 14703 | fbp54 | 0.0 | FBPs | Adherence | 99.34 | 100.00 | WP_011227179 |
| OLKLGFOO_15 | 77349 | 79133 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_011226325 |
| OLKLGFOO_13 | 80127 | 80858 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | WP_011681176 |
| OLKLGFOO_13 | 80127 | 80858 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011226048 |
| OLKLGFOO_13 | 146821 | 147611 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.74 | 100.00 | - |
| OLKLGFOO_13 | 80127 | 80858 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011227248 |
| OLKLGFOO_14 | 44358 | 45119 | srtA | 0.0 | Sortase A | Exoenzyme | 97.77 | 100.00 | - |
| OLKLGFOO_13 | 80859 | 82319 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.67 | 100.00 | WP_011681177 |
| OLKLGFOO_15 | 87806 | 88657 | rfbD | 0.0 | Capsule | Immune modulation | 96.60 | 100.00 | WP_014621820 |
| OLKLGFOO_13 | 80859 | 82319 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.51 | 100.21 | WP_011226049 |
| OLKLGFOO_13 | 78676 | 79416 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 95.82 | 100.00 | WP_011681174 |
| OLKLGFOO_13 | 80859 | 82319 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.69 | 100.21 | WP_041827043 |
| OLKLGFOO_13 | 78676 | 79416 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 95.41 | 100.00 | WP_011226046 |
| OLKLGFOO_13 | 78681 | 79416 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 95.11 | 98.13 | WP_011227246 |
| OLKLGFOO_13 | 79426 | 80118 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.09 | 100.00 | WP_011226047 |
| OLKLGFOO_13 | 79426 | 80118 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 94.81 | 100.00 | WP_011227247 |
| OLKLGFOO_13 | 77252 | 78619 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.74 | 99.93 | WP_011226045 |
| OLKLGFOO_13 | 77254 | 78619 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.37 | 99.71 | WP_011681173 |
| OLKLGFOO_13 | 79426 | 80118 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 92.35 | 100.00 | WP_011681175 |
| OLKLGFOO_15 | 85429 | 86703 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 91.14 | 100.00 | WP_011226331 |
| OLKLGFOO_2 | 39253 | 40556 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| OLKLGFOO_16 | 184 | 1194 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.36 | 99.80 | WP_002986042 |
| OLKLGFOO_23 | 6424 | 7707 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| OLKLGFOO_14 | 7523 | 8569 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| OLKLGFOO_13 | 68541 | 69124 | SPD_RS01775 | 1.14e-147 | Capsule | Immune modulation | 83.08 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OLKLGFOO_14 | 47806 | 48789 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| OLKLGFOO_5 | 24929 | 26002 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| OLKLGFOO_30 | 13296 | 14264 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| OLKLGFOO_15 | 261485 | 263788 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| OLKLGFOO_14 | 121076 | 122557 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| OLKLGFOO_5 | 45432 | 47678 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| OLKLGFOO_1 | 50129 | 51127 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| OLKLGFOO_1 | 4384 | 5355 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| OLKLGFOO_15 | 140053 | 138902 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| OLKLGFOO_15 | 103627 | 101639 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| OLKLGFOO_14 | 31609 | 29879 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| OLKLGFOO_25 | 64578 | 63100 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| OLKLGFOO_2 | 39253 | 40554 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| OLKLGFOO_25 | 49389 | 47497 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| OLKLGFOO_5 | 50603 | 48894 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| OLKLGFOO_17 | 21265 | 22275 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| OLKLGFOO_13 | 1505 | 144 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| OLKLGFOO_17 | 73308 | 71416 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| OLKLGFOO_2 | 24818 | 25708 | bglB | 8.08e-180 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| OLKLGFOO_9 | 11830 | 10892 | SP_0676 | 6.48e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| OLKLGFOO_5 | 47870 | 48727 | metF | 6.93e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| OLKLGFOO_13 | 119139 | 118435 | covR | 4.55e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| OLKLGFOO_14 | 102101 | 101430 | rr01 | 2.16e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| OLKLGFOO_5 | 54557 | 55225 | CiaR | 3.81e-124 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| OLKLGFOO_13 | 119139 | 118435 | walR | 1.92e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| OLKLGFOO_5 | 54557 | 55228 | ciaR | 4.05e-122 | two component system response regulator | 84.82 | 99.12 | AIK75708 |
| OLKLGFOO_25 | 183823 | 184509 | CovR | 1.26e-118 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| OLKLGFOO_1 | 51403 | 51777 | SpxA2 | 3.23e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| OLKLGFOO_13 | 12923 | 13324 | SpxA1 | 3.92e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| OLKLGFOO_25 | 206206 | 206493 | spr0479 | 1.77e-48 | essential gene | 86.46 | 98.97 | AAK99283 |
| OLKLGFOO_26 | 121770 | 122024 | spr1327 | 2.18e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| OLKLGFOO_25 | 15761 | 16024 | spr0175 | 8.94e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| OLKLGFOO_25 | 16588 | 16854 | spr0177 | 1.95e-33 | essential gene | 84.27 | 88.12 | AAK98981 |
| OLKLGFOO_23 | 7865 | 8365 | RopE (M5005_Spy_1611) | 1.78e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| OLKLGFOO_15 | 261485 | 263782 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| OLKLGFOO_17 | 58836 | 57817 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| OLKLGFOO_22 | 1615 | 2565 | rep37 | 956 / 945 | 87.97 | HM050421 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OLKLGFOO_1 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_2 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_3 | 1.0 | 0.0 | 1.0 |
| OLKLGFOO_4 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_5 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_6 | 1.0 | 0.0 | 1.0 |
| OLKLGFOO_7 | 1.0 | 0.0 | 1.0 |
| OLKLGFOO_8 | 1.0 | 0.0 | 1.0 |
| OLKLGFOO_9 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_10 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_11 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_12 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_13 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_14 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_15 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_16 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_17 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_18 | 1.0 | 0.0 | 1.0 |
| OLKLGFOO_19 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_20 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_22 | 1.0 | 0.0 | 1.0 |
| OLKLGFOO_23 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_24 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_25 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_26 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_27 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_28 | 0.0 | 1.0 | 0.0 |
| OLKLGFOO_29 | 1.0 | 0.0 | 1.0 |
| OLKLGFOO_30 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)