Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BBMIDLLD_1 | 3435432 | 3435749 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BBMIDLLD_1 | 3435094 | 3435432 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BBMIDLLD_1 | 2944154 | 2946724 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| BBMIDLLD_1 | 2602723 | 2604369 | vmlR | ARO:3004476 | vmlR | 98.91 | 100.18 | NC_000964.3 |
| BBMIDLLD_1 | 2349485 | 2350078 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| BBMIDLLD_1 | 2299011 | 2300444 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| BBMIDLLD_1 | 2286197 | 2287117 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| BBMIDLLD_1 | 418985 | 419839 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| BBMIDLLD_1 | 401892 | 403094 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| BBMIDLLD_1 | 181166 | 182335 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BBMIDLLD_1 | 418985 | 419839 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| BBMIDLLD_1 | 1949420 | 1950796 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.33 | 100.00 | D12567 |
| BBMIDLLD_1 | 2286197 | 2287117 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BBMIDLLD_1 | 2602726 | 2604369 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.91 | 100.00 | WP_003234144.1 |
| BBMIDLLD_1 | 418988 | 419839 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| BBMIDLLD_1 | 1949423 | 1950796 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.47 | 100.00 | WP_063856081.1 |
| BBMIDLLD_1 | 2286197 | 2287114 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| BBMIDLLD_1 | 1946931 | 1947449 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.33 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BBMIDLLD_1 | 4141373 | 4142014 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| BBMIDLLD_1 | 1453184 | 1454365 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BBMIDLLD_1 | 1013839 | 1014624 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BBMIDLLD_1 | 1012617 | 1013813 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| BBMIDLLD_1 | 3981736 | 3983499 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| BBMIDLLD_1 | 1451559 | 1452701 | capA | 0.0 | Capsule | Immune modulation | 98.69 | 100.00 | NP_391469 |
| BBMIDLLD_1 | 1452720 | 1453169 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| BBMIDLLD_1 | 1010003 | 1010941 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| BBMIDLLD_1 | 1002847 | 1009983 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.38 | 100.00 | NP_391076 |
| BBMIDLLD_1 | 1010969 | 1012588 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BBMIDLLD_1 | 2110173 | 2111309 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BBMIDLLD_1 | 1996358 | 1997227 | pdxS | 3.10e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BBMIDLLD_1 | 3744204 | 3744980 | CodY | 3.39e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BBMIDLLD_1 | 1294562 | 1295131 | ClpP | 2.51e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BBMIDLLD_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BBMIDLLD_1 | 352323 | 381896 | False | Myoviridae | VOG4705, VOG7718, VOG0906, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG7888, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG4693, VOG0189, VOG11060, VOG1309 |
| BBMIDLLD_1 | 557724 | 589737 | False | Siphoviridae | VOG4574, VOG0136, VOG5221, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841, VOG10057, VOG4018, VOG0198, VOG0044, VOG7372, VOG2752 |
| BBMIDLLD_1 | 2138372 | 2165149 | False | Siphoviridae | VOG4606, VOG7060, VOG2752, VOG10041, VOG7372, VOG0198, VOG4841, VOG7403, VOG4581, VOG4544, VOG3644, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG0641 |
| BBMIDLLD_1 | 2667094 | 2690188 | False | Myoviridae | VOG0720, VOG4555, VOG4553, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG5008, VOG0753, VOG0136, VOG4574 |
| BBMIDLLD_1 | 3377997 | 3405278 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)