Probiotic potential risk score


ARGs VFs PGs PPRS
2022.83

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes


Comprehensive Antibiotic Resistance Database (CARD)

Query ID Begin End ARO name ARO accession CARD name Identity Coverage Accession no.
HMKDLONG_171539417319tet(M)ARO:3000186tet(M)98.13100.31AB039845.1
HMKDLONG_171380815184tet(L)ARO:3000179tet(L)99.13100.00M11036.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID Begin End Resistance gene Phenotype Identity Coverage Accession no.
HMKDLONG_171380815184tet(L)Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline99.78100.00M29725
HMKDLONG_171539417319tet(M)Doxycycline, Tetracycline, Minocycline99.12100.00X75073

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID Begin End Gene symbol Sequence name Method Identity Coverage Accession no.
HMKDLONG_171381115184tet(L)tetracycline efflux MFS transporter Tet(L)BLASTX99.34100.00WP_001574277.1
HMKDLONG_171539717319tet(M)tetracycline resistance ribosomal protection protein Tet(M)BLASTX98.91100.00WP_000691736.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors


Virulence Factor Database (VFDB)

Query ID qstart qend Gene name e-value VF name VF category Identity Coverage Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID Begin End Database Virulence factor Protein function Identity Coverage Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes


PHI-base

Query ID qstart qend Gene name e-value Function Identity Coverage Gene ID
HMKDLONG_92818827481WalR2.18e-116transcriptional regulatory protein85.59100.43EPH95667
HMKDLONG_29703596838CspR3.87e-28cold shock protein81.82100.00AAO82613
HMKDLONG_29703596841CspA1.89e-27cold shock protein81.5498.48CAA62903

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid


PlasmidFinder

Query ID Begin End Plasmid Query / Template Identity Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID Prediction Chromosome Plasmid
HMKDLONG_10.01.00.0
HMKDLONG_20.01.00.0
HMKDLONG_31.00.01.0
HMKDLONG_40.01.00.0
HMKDLONG_51.00.01.0
HMKDLONG_60.01.00.0
HMKDLONG_70.01.00.0
HMKDLONG_80.01.00.0
HMKDLONG_90.01.00.0
HMKDLONG_100.01.00.0
HMKDLONG_111.00.01.0
HMKDLONG_120.01.00.0
HMKDLONG_130.01.00.0
HMKDLONG_140.01.00.0
HMKDLONG_150.01.00.0
HMKDLONG_161.00.01.0
HMKDLONG_171.00.01.0
HMKDLONG_180.01.00.0
HMKDLONG_190.01.00.0
HMKDLONG_200.01.00.0
HMKDLONG_221.00.01.0
HMKDLONG_231.00.01.0
HMKDLONG_251.00.01.0
HMKDLONG_271.00.01.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage


Phigaro

Query ID Begin End Transposable Taxonomy pVOGs
HMKDLONG_35715760017FalseUnknownVOG3349, VOG2788, VOG3622, VOG0267, VOG0267, VOG0267, VOG3349, VOG8288, VOG0267
HMKDLONG_11479137744FalseSiphoviridaeVOG4334, VOG4763, VOG10868, VOG4856, VOG3462, VOG0801, VOG5793, VOG0866, VOG9312, VOG4586, VOG0207, VOG9583, VOG0205, VOG0204, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841, VOG8681, VOG0198, VOG2246, VOG5795, VOG0327, VOG4609, VOG4632, VOG6545, VOG0322, VOG4548, VOG0321, VOG6357, VOG9747

Phigaro v2.4.0 (Database: Jan. 2024 release)


Insertion sequence (IS) elements