Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 1 | 4 | 6.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BIGIONGN_3 | 408016 | 408663 | Alkalihalobacillus clausii chloramphenicol acetyltransferase | ARO:3004452 | Acla_ACT_CHL | 88.68 | 94.30 | AY238971.1 |
| BIGIONGN_5 | 324598 | 325443 | Erm(34) | ARO:3000600 | Erm(34) | 96.80 | 100.00 | AY234334.1 |
| BIGIONGN_17 | 59485 | 60255 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 99.22 | 100.00 | EF540343.1 |
| BIGIONGN_17 | 56166 | 57090 | BCL-1 | ARO:3004749 | BCL-1 | 100.00 | 98.05 | EF540343.1 |
| BIGIONGN_11 | 16247 | 17299 | clbC | ARO:3002816 | clbC | 100.00 | 100.00 | AP006627.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BIGIONGN_17 | 59485 | 60255 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 99.09 | 100.00 | AJ506108 |
| BIGIONGN_3 | 407985 | 408661 | cat | Chloramphenicol | 97.52 | 98.40 | AY238971 |
| BIGIONGN_5 | 324605 | 325443 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 95.47 | 99.17 | AY234334 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BIGIONGN_17 | 56169 | 57071 | blaBCL-1 | class A beta-lactamase BCL-1 | ALLELEX | 100.00 | 100.00 | WP_065825670.1 |
| BIGIONGN_3 | 408019 | 408663 | catA | type A-10 chloramphenicol O-acetyltransferase | EXACTX | 100.00 | 100.00 | WP_035203840.1 |
| BIGIONGN_11 | 16247 | 17296 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | EXACTX | 100.00 | 100.00 | WP_011245929.1 |
| BIGIONGN_17 | 59488 | 60255 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 99.22 | 100.00 | WP_035202393.1 |
| BIGIONGN_5 | 324604 | 325443 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 97.14 | 99.64 | WP_063844504.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BIGIONGN_2 | 130292 | 130942 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BIGIONGN_16 | 35617 | 36753 | tufA | 0.0 | elongation factor Tu | 87.33 | 95.69 | AHM69299 |
| BIGIONGN_15 | 36727 | 36969 | SpoVG | 2.57e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BIGIONGN_1 | 29417 | 29223 | CspA | 2.98e-29 | cold shock protein | 86.15 | 98.48 | CAA62903 |
| BIGIONGN_2 | 611024 | 610833 | msaB | 1.74e-25 | cold shock protein | 81.25 | 96.97 | ABD22065 |
| BIGIONGN_2 | 611021 | 610833 | CspB | 1.49e-24 | cold shock protein | 80.95 | 95.45 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BIGIONGN_1 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_2 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_3 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_4 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_5 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_6 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_7 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_8 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_9 | 1.0 | 0.0 | 1.0 |
| BIGIONGN_10 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_11 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_12 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_13 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_14 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_15 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_16 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_17 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_18 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_19 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_20 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_21 | 0.0 | 1.0 | 0.0 |
| BIGIONGN_22 | 1.0 | 0.0 | 1.0 |
| BIGIONGN_23 | 1.0 | 0.0 | 1.0 |
| BIGIONGN_26 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BIGIONGN_7 | 78422 | 110434 | False | Myoviridae | VOG8979, VOG0992, VOG0602, VOG0753, VOG4705, VOG3390, VOG5384, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG4910, VOG3892, VOG3891, VOG3890, VOG10055, VOG5660, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG7407, VOG0796, VOG5447 |
| BIGIONGN_9 | 55411 | 92917 | False | Myoviridae / Siphoviridae | VOG0083, VOG5722, VOG5779, VOG5721, VOG10940, VOG7134, VOG6941, VOG6036, VOG9219, VOG6472, VOG9713, VOG6940, VOG5534, VOG5601, VOG0537, VOG7930, VOG0275, VOG9667, VOG11003, VOG1070, VOG4779, VOG3389, VOG5042, VOG3498, VOG9712 |
| BIGIONGN_9 | 154237 | 167763 | False | Myoviridae / Siphoviridae | VOG4724, VOG1198, VOG1198, VOG0918, VOG4551, VOG0025, VOG7476, VOG0322, VOG6211 |
Phigaro v2.4.0 (Database: Jan. 2024 release)