Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 6 | 1 | 4 | 7.28 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JMBIACCC_4 | 597216 | 598061 | Erm(34) | ARO:3000600 | Erm(34) | 96.80 | 100.00 | AY234334.1 |
| JMBIACCC_28 | 368000 | 368924 | BCL-1 | ARO:3004749 | BCL-1 | 100.00 | 98.05 | EF540343.1 |
| JMBIACCC_28 | 364835 | 365605 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 100.00 | 100.00 | EF540343.1 |
| JMBIACCC_11 | 274851 | 275903 | clbC | ARO:3002816 | clbC | 100.00 | 100.00 | AP006627.1 |
| JMBIACCC_3 | 81220 | 81867 | Alkalihalobacillus clausii chloramphenicol acetyltransferase | ARO:3004452 | Acla_ACT_CHL | 88.68 | 94.30 | AY238971.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| JMBIACCC_28 | 364835 | 365605 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 100.00 | 100.00 | AJ506108 |
| JMBIACCC_3 | 81222 | 81898 | cat | Chloramphenicol | 97.52 | 98.40 | AY238971 |
| JMBIACCC_4 | 597223 | 598061 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 95.47 | 99.17 | AY234334 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JMBIACCC_28 | 364835 | 365602 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | EXACTX | 100.00 | 100.00 | WP_035202393.1 |
| JMBIACCC_28 | 368018 | 368920 | blaBCL-1 | class A beta-lactamase BCL-1 | ALLELEX | 100.00 | 100.00 | WP_065825670.1 |
| JMBIACCC_3 | 81220 | 81864 | catA | type A-10 chloramphenicol O-acetyltransferase | EXACTX | 100.00 | 100.00 | WP_035203840.1 |
| JMBIACCC_11 | 274854 | 275903 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | EXACTX | 100.00 | 100.00 | WP_011245929.1 |
| JMBIACCC_4 | 597222 | 598061 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 97.14 | 99.64 | WP_063844504.1 |
| JMBIACCC_4 | 699606 | 702197 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.14 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JMBIACCC_8 | 317377 | 318027 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JMBIACCC_1 | 32821 | 33957 | tufA | 0.0 | elongation factor Tu | 87.33 | 95.69 | AHM69299 |
| JMBIACCC_7 | 36610 | 36852 | SpoVG | 2.57e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| JMBIACCC_2 | 29427 | 29233 | CspA | 2.89e-29 | cold shock protein | 86.15 | 98.48 | CAA62903 |
| JMBIACCC_4 | 149245 | 149054 | msaB | 2.25e-25 | cold shock protein | 81.25 | 96.97 | ABD22065 |
| JMBIACCC_4 | 149242 | 149054 | CspB | 1.93e-24 | cold shock protein | 80.95 | 95.45 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JMBIACCC_1 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_2 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_3 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_4 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_5 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_6 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_7 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_8 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_9 | 1.0 | 0.0 | 1.0 |
| JMBIACCC_11 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_12 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_13 | 1.0 | 0.0 | 1.0 |
| JMBIACCC_14 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_15 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_16 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_18 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_19 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_20 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_27 | 0.0 | 1.0 | 0.0 |
| JMBIACCC_28 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JMBIACCC_28 | 264100 | 293182 | False | Siphoviridae | VOG6242, VOG5447, VOG4585, VOG0796, VOG0720, VOG4564, VOG4555, VOG3434, VOG1887, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG6163, VOG0801, VOG4619, VOG0565, VOG4669, VOG0753, VOG0602, VOG4724, VOG6091 |
Phigaro v2.4.0 (Database: Jan. 2024 release)