safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
14	10	3	17.46

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
POFEPANA_1	3539392	3540042	Streptococcus suis chloramphenicol acetyltransferase	ARO:3004455	Ssui_ACT_CHL	94.91	100.00	AB080798.1
POFEPANA_1	2714849	2715703	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
POFEPANA_1	2696140	2697342	blt	ARO:3003006	blt	100.00	100.00	L32599.1
POFEPANA_1	2473822	2474991	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
POFEPANA_1	1355993	1356307	ykkD	ARO:3003064	ykkD	99.05	99.05	AL009126.1
POFEPANA_1	1355655	1355993	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
POFEPANA_1	895850	898420	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
POFEPANA_1	583811	585454	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
POFEPANA_1	338743	339336	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
POFEPANA_1	288240	289673	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
POFEPANA_1	275425	276345	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
POFEPANA_1	2714849	2715703	aadK	Streptomycin	100.00	100.00	M26879
POFEPANA_1	3539392	3540042	cat(pC194)	Chloramphenicol	100.00	100.00	NC_002013
POFEPANA_1	275425	276345	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
POFEPANA_1	4178713	4180089	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
POFEPANA_1	2714852	2715703	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
POFEPANA_1	3539392	3540039	catA	type A-9 chloramphenicol O-acetyltransferase	EXACTX	100.00	100.00	WP_001010387.1
POFEPANA_1	275425	276342	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
POFEPANA_1	4176195	4176713	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
POFEPANA_1	4178716	4180089	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
POFEPANA_1	583814	585454	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
POFEPANA_1	2030505	2033099	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
POFEPANA_1	1983847	1985610	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
POFEPANA_1	3270723	3271508	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
POFEPANA_1	3266887	3267825	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
POFEPANA_1	3269501	3270697	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
POFEPANA_1	3267853	3269472	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
POFEPANA_1	3259731	3266867	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
POFEPANA_1	2274470	2275111	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062
POFEPANA_1	3679626	3680807	capB	Capsule	Immune modulation	99.66	100.00	NP_391471
POFEPANA_1	3678001	3679143	capA	Capsule	Immune modulation	99.30	100.00	NP_391469
POFEPANA_1	3679162	3679611	capC	Capsule	Immune modulation	98.89	100.00	NP_391470

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
POFEPANA_1	132473	133609	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
POFEPANA_1	18665	19534	pdxS	3.12e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
POFEPANA_1	3525447	3526016	ClpP	2.53e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
POFEPANA_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
POFEPANA_1	1299708	1325816	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
POFEPANA_1	2646212	2676456	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)