safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
KODMPLFM_1	2701496	2702350	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
KODMPLFM_1	2682787	2683989	blt	ARO:3003006	blt	100.00	100.00	L32599.1
KODMPLFM_1	2460469	2461638	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
KODMPLFM_1	1342640	1342954	ykkD	ARO:3003064	ykkD	99.05	99.05	AL009126.1
KODMPLFM_1	1342302	1342640	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
KODMPLFM_1	882495	885065	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
KODMPLFM_1	570457	572100	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
KODMPLFM_1	325509	326102	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
KODMPLFM_1	277653	279086	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
KODMPLFM_1	264838	265758	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
KODMPLFM_1	2701496	2702350	aadK	Streptomycin	100.00	100.00	M26879
KODMPLFM_1	264838	265758	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
KODMPLFM_1	4164289	4165665	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
KODMPLFM_1	2701499	2702350	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
KODMPLFM_1	264838	265755	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
KODMPLFM_1	4161771	4162289	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
KODMPLFM_1	4164292	4165665	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
KODMPLFM_1	570460	572100	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
KODMPLFM_1	2017152	2019746	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
KODMPLFM_1	1970494	1972257	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
KODMPLFM_1	3257332	3258117	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
KODMPLFM_1	3253496	3254434	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
KODMPLFM_1	3256110	3257306	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
KODMPLFM_1	3254462	3256081	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
KODMPLFM_1	3246340	3253476	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
KODMPLFM_1	2261117	2261758	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062
KODMPLFM_1	3665238	3666419	capB	Capsule	Immune modulation	99.66	100.00	NP_391471
KODMPLFM_1	3663613	3664755	capA	Capsule	Immune modulation	99.30	100.00	NP_391469
KODMPLFM_1	3664774	3665223	capC	Capsule	Immune modulation	98.89	100.00	NP_391470

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
KODMPLFM_1	132473	133609	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
KODMPLFM_1	18665	19534	pdxS	3.11e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
KODMPLFM_1	1655938	1656714	CodY	3.40e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
KODMPLFM_1	3512045	3512614	ClpP	2.52e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
KODMPLFM_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
KODMPLFM_1	1286355	1312463	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
KODMPLFM_1	2632859	2663103	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)