safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
AOBGNAGB_1	2735273	2736127	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
AOBGNAGB_1	2716564	2717766	blt	ARO:3003006	blt	100.00	100.00	L32599.1
AOBGNAGB_1	2494245	2495414	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
AOBGNAGB_1	1376449	1376766	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
AOBGNAGB_1	1376111	1376449	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
AOBGNAGB_1	916367	918937	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
AOBGNAGB_1	604332	605975	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
AOBGNAGB_1	338754	339347	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
AOBGNAGB_1	288252	289685	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
AOBGNAGB_1	275437	276357	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
AOBGNAGB_1	2735273	2736127	aadK	Streptomycin	100.00	100.00	M26879
AOBGNAGB_1	275437	276357	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
AOBGNAGB_1	4187300	4188676	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
AOBGNAGB_1	2735276	2736127	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
AOBGNAGB_1	275437	276354	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
AOBGNAGB_1	4184782	4185300	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
AOBGNAGB_1	4187303	4188676	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
AOBGNAGB_1	604335	605975	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
AOBGNAGB_1	2050934	2053528	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
AOBGNAGB_1	3697439	3698581	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
AOBGNAGB_1	3699064	3700245	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
AOBGNAGB_1	3698600	3699049	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
AOBGNAGB_1	2004276	2006039	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
AOBGNAGB_1	3291103	3291888	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
AOBGNAGB_1	3287267	3288205	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
AOBGNAGB_1	3289881	3291077	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
AOBGNAGB_1	3288233	3289852	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
AOBGNAGB_1	3280111	3287247	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
AOBGNAGB_1	2294899	2295540	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
AOBGNAGB_1	132473	133609	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
AOBGNAGB_1	18665	19534	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
AOBGNAGB_1	1689717	1690493	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
AOBGNAGB_1	3545827	3546396	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
AOBGNAGB_1	0.0	1.0	0.0
AOBGNAGB_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
AOBGNAGB_1	1320164	1346272	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
AOBGNAGB_1	2666635	2696879	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600
AOBGNAGB_2	4939	15776	False	Siphoviridae	VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG0221
AOBGNAGB_2	43831	56929	False	Unknown	VOG4549, VOG2725, VOG3851, VOG2352, VOG4714, VOG4561, VOG1198, VOG0397, VOG2352, VOG11080

Phigaro v2.4.0 (Database: Jan. 2024 release)