safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
1	0	0	1.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
LFPPBNBB_1	629005	629649	vatE	ARO:3002844	vatE	100.00	100.00	AF242872.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
LFPPBNBB_1	629005	629649	vat(E)	Dalfopristin, Pristinamycin IIA, Virginiamycin M	100.00	100.00	AJ488494

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
LFPPBNBB_1	629005	629646	vat(E)	streptogramin A O-acetyltransferase Vat(E)	EXACTX	100.00	100.00	WP_002328813.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
LFPPBNBB_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
LFPPBNBB_1	597293	607956	False	Siphoviridae	VOG5818, VOG0918, VOG7188, VOG8910, VOG0275, VOG0862, VOG9667, VOG1563, VOG0181, VOG4799
LFPPBNBB_1	772347	779325	False	Myoviridae / Siphoviridae / Podoviridae	VOG0707, VOG3478, VOG5692, VOG4955, VOG0226, VOG0514, VOG4908, VOG7910, VOG9078, VOG3307, VOG0686, VOG1183, VOG6495
LFPPBNBB_1	804907	832635	False	Siphoviridae	VOG0866, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG4545, VOG0801, VOG3462, VOG10972, VOG1050, VOG9997, VOG4856, VOG4334, VOG4705
LFPPBNBB_1	958099	1000562	False	Siphoviridae	VOG6495, VOG4602, VOG9667, VOG7581, VOG2776, VOG1638, VOG5878, VOG7341, VOG0535, VOG0322, VOG0226, VOG0514, VOG0549, VOG0550, VOG0189, VOG0536, VOG4552, VOG1298, VOG3307, VOG0686, VOG1183, VOG4693, VOG6140, VOG0198, VOG9888, VOG0689, VOG9503, VOG0796, VOG4544, VOG0720, VOG4564, VOG4555, VOG3434, VOG1887, VOG4713, VOG1330, VOG1900, VOG0704, VOG4634, VOG1333, VOG1334, VOG0083, VOG6163, VOG1335, VOG10608, VOG9888, VOG0648, VOG0641
LFPPBNBB_1	1029239	1057042	False	Siphoviridae	VOG0198, VOG4841, VOG4581, VOG9312, VOG0866, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG0207, VOG0209, VOG6163, VOG0801, VOG3462, VOG10972, VOG4357, VOG10972, VOG1050, VOG9997, VOG4856, VOG4334, VOG4705, VOG7888
LFPPBNBB_1	1264144	1290782	True	Siphoviridae	VOG2621, VOG4612, VOG3462, VOG0801, VOG4545, VOG0725, VOG0799, VOG0724, VOG1353, VOG4713, VOG1329, VOG4555, VOG0638, VOG4564, VOG0720, VOG10227, VOG3699, VOG0796, VOG4357, VOG4357, VOG3594, VOG0198, VOG4039, VOG4693
LFPPBNBB_1	1550780	1558155	False	Siphoviridae	VOG0350, VOG1844, VOG4553, VOG0204, VOG4589, VOG0641, VOG9409