Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 33 | 41.40 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EKFBEHAF_29 | 1543 | 2136 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| EKFBEHAF_47 | 2389 | 3849 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 99.93 | 100.00 | WP_011681177 |
| EKFBEHAF_29 | 1543 | 2136 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| EKFBEHAF_24 | 186062 | 186871 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_002948020 |
| EKFBEHAF_29 | 2136 | 3005 | rfbA | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011681286 |
| EKFBEHAF_29 | 1543 | 2136 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| EKFBEHAF_57 | 114651 | 116309 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| EKFBEHAF_24 | 185122 | 186078 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226328 |
| EKFBEHAF_44 | 2262 | 3405 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.56 | 100.00 | - |
| EKFBEHAF_21 | 16469 | 17308 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| EKFBEHAF_24 | 189160 | 190944 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.44 | 100.00 | WP_011226325 |
| EKFBEHAF_24 | 186871 | 188076 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.17 | 100.00 | WP_011226326 |
| EKFBEHAF_24 | 178084 | 178935 | rfbD | 0.0 | Capsule | Immune modulation | 99.06 | 100.00 | WP_014621820 |
| EKFBEHAF_47 | 1657 | 2388 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| EKFBEHAF_19 | 14580 | 15341 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| EKFBEHAF_24 | 183977 | 185125 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | WP_041828290 |
| EKFBEHAF_47 | 1657 | 2388 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011227248 |
| EKFBEHAF_47 | 1657 | 2388 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011226048 |
| EKFBEHAF_57 | 8416 | 9831 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.23 | 100.00 | WP_011681165 |
| EKFBEHAF_47 | 206 | 946 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.11 | 100.00 | WP_011226046 |
| EKFBEHAF_47 | 206 | 946 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011681174 |
| EKFBEHAF_47 | 2389 | 3849 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.40 | 100.21 | WP_011226049 |
| EKFBEHAF_47 | 211 | 946 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.15 | 98.13 | WP_011227246 |
| EKFBEHAF_57 | 1 | 1274 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 97.02 | 93.13 | WP_011226045 |
| EKFBEHAF_47 | 2389 | 3849 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.85 | 100.21 | WP_041827043 |
| EKFBEHAF_47 | 956 | 1648 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.38 | 100.00 | WP_011227247 |
| EKFBEHAF_47 | 956 | 1648 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.38 | 100.00 | WP_011226047 |
| EKFBEHAF_24 | 188096 | 189163 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 95.27 | 101.14 | WP_002948022 |
| EKFBEHAF_57 | 1 | 1272 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.34 | 92.98 | WP_011681173 |
| EKFBEHAF_47 | 956 | 1648 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.48 | 100.00 | WP_011681175 |
| EKFBEHAF_24 | 180038 | 181312 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 89.42 | 99.92 | WP_011226331 |
| EKFBEHAF_54 | 22108 | 23411 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.02 | 99.46 | WP_000022832 |
| EKFBEHAF_31 | 49 | 1059 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| EKFBEHAF_35 | 159415 | 160698 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.81 | 99.77 | WP_000107753 |
| EKFBEHAF_29 | 68 | 1114 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| EKFBEHAF_57 | 11651 | 12234 | SPD_RS01775 | 7.06e-153 | Capsule | Immune modulation | 83.59 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EKFBEHAF_19 | 18028 | 19011 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| EKFBEHAF_59 | 13726 | 12653 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| EKFBEHAF_24 | 15527 | 13224 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| EKFBEHAF_54 | 107049 | 106081 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| EKFBEHAF_27 | 11292 | 9046 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| EKFBEHAF_15 | 12541 | 11060 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.70 | 100.00 | ABJ54480 |
| EKFBEHAF_21 | 36100 | 37098 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| EKFBEHAF_21 | 1 | 957 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.60 | 98.46 | ABF36968 |
| EKFBEHAF_24 | 129544 | 130695 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| EKFBEHAF_24 | 164907 | 166895 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| EKFBEHAF_19 | 1829 | 99 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| EKFBEHAF_16 | 28051 | 26573 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| EKFBEHAF_54 | 23411 | 22110 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| EKFBEHAF_16 | 12862 | 10970 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| EKFBEHAF_27 | 6121 | 7830 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| EKFBEHAF_61 | 54797 | 53787 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| EKFBEHAF_57 | 92797 | 94158 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| EKFBEHAF_61 | 4453 | 6345 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| EKFBEHAF_54 | 37847 | 36957 | bglB | 3.39e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| EKFBEHAF_17 | 11491 | 10553 | SP_0676 | 5.62e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| EKFBEHAF_27 | 8854 | 7997 | metF | 1.99e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| EKFBEHAF_62 | 29780 | 30484 | covR | 1.56e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| EKFBEHAF_15 | 31516 | 32187 | rr01 | 9.66e-126 | response regulator | 83.93 | 99.56 | CAB54566 |
| EKFBEHAF_62 | 29780 | 30484 | walR | 6.59e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| EKFBEHAF_27 | 2167 | 1499 | CiaR | 2.64e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| EKFBEHAF_35 | 7669 | 6983 | CovR | 9.04e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| EKFBEHAF_27 | 2167 | 1496 | ciaR | 2.60e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| EKFBEHAF_21 | 37374 | 37748 | SpxA2 | 2.35e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| EKFBEHAF_57 | 81435 | 81034 | SpxA1 | 2.44e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| EKFBEHAF_20 | 13647 | 13934 | spr0479 | 2.98e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| EKFBEHAF_45 | 5650 | 5396 | spr1327 | 1.88e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| EKFBEHAF_22 | 20882 | 20619 | spr0175 | 1.40e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| EKFBEHAF_22 | 20055 | 19789 | spr0177 | 3.06e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| EKFBEHAF_35 | 159257 | 158757 | RopE (M5005_Spy_1611) | 4.43e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| EKFBEHAF_24 | 15527 | 13230 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| EKFBEHAF_61 | 18913 | 19932 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EKFBEHAF_6 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_15 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_16 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_17 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_18 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_19 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_20 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_21 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_22 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_23 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_24 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_25 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_26 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_27 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_28 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_29 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_30 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_31 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_32 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_33 | 1.0 | 0.0 | 1.0 |
| EKFBEHAF_34 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_35 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_36 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_37 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_38 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_39 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_40 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_41 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_42 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_43 | 1.0 | 0.0 | 1.0 |
| EKFBEHAF_44 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_45 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_46 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_47 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_48 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_49 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_50 | 1.0 | 0.0 | 1.0 |
| EKFBEHAF_51 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_52 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_53 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_54 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_57 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_58 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_59 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_61 | 0.0 | 1.0 | 0.0 |
| EKFBEHAF_62 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)